Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
Key features and details
- Mouse monoclonal [PCRP-RXRB-2B6] to Retinoid X Receptor beta/RXRB - BSA and Azide free
- Suitable for: Protein Array, IHC-P, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG2a
Overview
-
Product name
Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free
See all Retinoid X Receptor beta/RXRB primary antibodies -
Description
Mouse monoclonal [PCRP-RXRB-2B6] to Retinoid X Receptor beta/RXRB - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-P, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Retinoid X Receptor beta/RXRB.
Database link: P28702 -
Positive control
- Flow cyt: U87, HeLa cells. ICC: HeLa, K562 cells. IHC-P: Human lymph node tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate -
Clonality
Monoclonal -
Clone number
PCRP-RXRB-2B6 -
Isotype
IgG2a -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab277119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Protein Array |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Flow Cyt |
Use at an assay dependent concentration.
|
|
ICC |
Use at an assay dependent concentration.
|
Notes |
---|
Protein Array
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Target
-
Function
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE). -
Tissue specificity
Expressed in a variety of tumor cell lines. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6257 Human
- Omim: 180246 Human
- SwissProt: P28702 Human
- Unigene: 388034 Human
-
Alternative names
- DAUD I6 antibody
- DAUDI6 antibody
- H 2RIIBP antibody
see all
Images
-
Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using ab277104, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling Retinoid X Receptor beta/RXRB with ab277104 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
-
Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using ab277104, the same antibody clone in a different buffer formulation.
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB using ab277104 at 2 µg/106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
-
Flow Cytometry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using ab277104, the same antibody clone in a different buffer formulation.
Flow cytometry analysis of PFA-fixed U87 (human glioblastoma-astrocytoma epithelial cell line) cells labeling Retinoid X Receptor beta/RXRB using ab277104 at 2 µg/106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
-
Immunocytochemistry - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using ab277104, the same antibody clone in a different buffer formulation.
Immunofluorescence analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Retinoid X Receptor beta/RXRB with ab277104 at 2 µg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
-
Protein Array - Anti-Retinoid X Receptor beta/RXRB antibody [PCRP-RXRB-2B6] - BSA and Azide free (ab277119)
This data was developed using ab277104, the same antibody clone in a different buffer formulation.
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277104.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
References (0)
ab277119 has not yet been referenced specifically in any publications.