Recombinant Anti-RHAG antibody [EPR10011] (ab155094)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10011] to RHAG
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-RHAG antibody [EPR10011]
See all RHAG primary antibodies -
Description
Rabbit monoclonal [EPR10011] to RHAG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human RHAG aa 350 to the C-terminus.
Database link: Q02094 -
Positive control
- Human fetal kidney, fetal liver and bone marrow lysates; Human lung and spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10011 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155094 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Relevance
RHAG (Rhesus blood group-associated glycoprotein) is associated with rhesus blood group antigen expression. It may be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. The Rh blood group antigens are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. Heterogeneously glycosylated membrane proteins of 50 and 45 kD, the Rh50 glycoproteins, are coprecipitated with the Rh30 polypeptides on immunoprecipitation with anti-Rh-specific mono- and polyclonal antibodies. The Rh antigens appear to exist as a multisubunit complex of CD47, LW, glycophorin B and play a critical role in the Rh50 glycoprotein. -
Cellular localization
Cell Membrane; multi-pass membrane protein -
Database links
- Entrez Gene: 6005 Human
- Entrez Gene: 19743 Mouse
- Entrez Gene: 65207 Rat
- Omim: 180297 Human
- SwissProt: Q02094 Human
- SwissProt: Q9QUT0 Mouse
- SwissProt: Q7TNK7 Rat
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Alternative names
- Abnormal Rhesus blood group associated glycoprotein antibody
- Ammonium transporter Rh type A antibody
- CD241 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
ab155094 showing +ve staining in Human normal tonsil.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
ab155094 showing +ve staining in Human normal heart.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
ab155094 showing +ve staining in Human normal colon.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-RHAG antibody [EPR10011] (ab155094) at 1/1000 dilution
Lane 1 : Human fetal kidney cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : Human bone marrow cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling RHAG with ab155094 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling RHAG with ab155094 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155094 has been referenced in 2 publications.
- Xia RW et al. A novel double-variant RHAG allele leads to Rhmod phenotype. Transfus Med N/A:N/A (2019). PubMed: 31032541
- Siau A et al. Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. Sci Rep 6:31055 (2016). PubMed: 27503796