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RecombinantRabMAb

Recombinant Anti-RHAG antibody [EPR10011] (ab155094)

Submit a review Submit a question References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
  • Western blot - Anti-RHAG antibody [EPR10011] (ab155094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
  • Anti-RHAG antibody [EPR10011] (ab155094)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10011] to RHAG
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-RHAG antibody [EPR10011]
    See all RHAG primary antibodies

  • Description

    Rabbit monoclonal [EPR10011] to RHAG

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human RHAG aa 350 to the C-terminus.
    Database link: Q02094

  • Positive control

    • Human fetal kidney, fetal liver and bone marrow lysates; Human lung and spleen tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20ºC.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10011

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Relevance

    RHAG (Rhesus blood group-associated glycoprotein) is associated with rhesus blood group antigen expression. It may be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. The Rh blood group antigens are associated with human erythrocyte membrane proteins of approximately 30 kD, the so-called Rh30 polypeptides. Heterogeneously glycosylated membrane proteins of 50 and 45 kD, the Rh50 glycoproteins, are coprecipitated with the Rh30 polypeptides on immunoprecipitation with anti-Rh-specific mono- and polyclonal antibodies. The Rh antigens appear to exist as a multisubunit complex of CD47, LW, glycophorin B and play a critical role in the Rh50 glycoprotein.

  • Cellular localization

    Cell Membrane; multi-pass membrane protein

  • Database links

    • Alternative names

      • Abnormal Rhesus blood group associated glycoprotein antibody
      • Ammonium transporter Rh type A antibody
      • CD241 antibody
      • CD241 antigen antibody
      • Erythrocyte membrane glycoprotein Rh50 antibody
      • Erythrocyte plasma membrane 50 kDa glycoprotein antibody
      • Rh associated glycoprotein antibody
      • Rh family type A glycoprotein antibody
      • Rh type A glycoprotein antibody
      • RH2 antibody
      • RH50 antibody
      • Rh50 glycoprotein antibody
      • Rh50 GP antibody
      • RH50A antibody
      • Rh50GP antibody
      • Rhesus associated polypeptide, 50-KD antibody
      • Rhesus blood group associated ammonia channel antibody
      • Rhesus blood group associated glycoprotein antibody
      • Rhesus blood group family type A glycoprotein antibody
      • SLC42A1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)

      ab155094 showing +ve staining in Human normal tonsil.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)

      ab155094 showing +ve staining in Human normal heart.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)

      ab155094 showing +ve staining in Human normal colon.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-RHAG antibody [EPR10011] (ab155094)
      Western blot - Anti-RHAG antibody [EPR10011] (ab155094)
      All lanes : Anti-RHAG antibody [EPR10011] (ab155094) at 1/1000 dilution

      Lane 1 : Human fetal kidney cell lysate
      Lane 2 : Human fetal liver cell lysate
      Lane 3 : Human bone marrow cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)

      Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling RHAG with ab155094 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RHAG antibody [EPR10011] (ab155094)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling RHAG with ab155094 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-RHAG antibody [EPR10011] (ab155094)
      Anti-RHAG antibody [EPR10011] (ab155094)

    References (2)

    Publishing research using ab155094? Please let us know so that we can cite the reference in this datasheet.

    ab155094 has been referenced in 2 publications.

    • Xia RW  et al. A novel double-variant RHAG allele leads to Rhmod phenotype. Transfus Med N/A:N/A (2019). PubMed: 31032541
    • Siau A  et al. Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. Sci Rep 6:31055 (2016). PubMed: 27503796

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