Recombinant Anti-RhD antibody [D7C2] (ab281912)
![Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] (ab281912)](https://www.abcam.com/ps/products/281/ab281912/Images/ab281912-1-AntiRhD-antibody-immunohistochemistry-K562-cells.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] (ab281912)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [D7C2] to RhD
- Suitable for: ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RhD antibody [D7C2]
See all RhD primary antibodies -
Description
Human monoclonal [D7C2] to RhD -
Host species
Human -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human RhD. Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r). Isolated peripheral lymphocytes were transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Database link: Q02161 -
Positive control
- ICC/IF: K562 cells.
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General notes
ab281912 is the recombinant human IgM version of ab281911.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
D7C2 -
Isotype
IgM -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. -
Tissue specificity
Restricted to tissues or cell lines expressing erythroid characters. -
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6007 Human
- Omim: 111680 Human
- SwissProt: Q02161 Human
- Unigene: 449968 Human
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Alternative names
- Blood group Rh(D) polypeptide antibody
- Blood group--Rhesus system D polypeptide antibody
- CD240D antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] (ab281912)
This data was developed using the chimeric version (ab281911).
Immunofluorescence analysis of paraformaldehyde fixed K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling RhD with ab281911 at 10 µg/ml for 1hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. K562 cells were immobilized on Shi-fix™ coverslips.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab281912 has not yet been referenced specifically in any publications.