Recombinant Anti-RhD antibody [D7C2] - Chimeric (ab281911)
![Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] - Chimeric (ab281911)](https://www.abcam.com/ps/products/281/ab281911/Images/ab281911-1-AntiRhD-antibody-immunohistochemistry-K562-cells.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] - Chimeric (ab281911)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [D7C2] to RhD - Chimeric
- Suitable for: ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-RhD antibody [D7C2] - Chimeric
See all RhD primary antibodies -
Description
Rabbit monoclonal [D7C2] to RhD - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human RhD. Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r). Isolated peripheral lymphocytes were transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Database link: Q02161 -
Positive control
- ICC/IF: K562 cells.
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General notes
ab281911 is the chimeric rabbit version of ab281912.
This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgM format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
D7C2 -
Isotype
IgG -
Light chain type
lambda -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. -
Tissue specificity
Restricted to tissues or cell lines expressing erythroid characters. -
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6007 Human
- Omim: 111680 Human
- SwissProt: Q02161 Human
- Unigene: 449968 Human
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Alternative names
- Blood group Rh(D) polypeptide antibody
- Blood group--Rhesus system D polypeptide antibody
- CD240D antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-RhD antibody [D7C2] - Chimeric (ab281911)
Immunofluorescence analysis of paraformaldehyde fixed K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling RhD with ab281911 at 10 µg/ml for 1hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. K562 cells were immobilized on Shi-fix™ coverslips.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab281911 has not yet been referenced specifically in any publications.