Recombinant Anti-Rho A + B + C antibody [EPR18299] (ab188103)
![Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242941-anti-rho-a-b-c-antibody-epr18299-western-blot.jpg "Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18299] to Rho A + B + C
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Rho A + B + C antibody [EPR18299]
See all Rho A + B + C primary antibodies -
Description
Rabbit monoclonal [EPR18299] to Rho A + B + C -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human RhoA, RhoB and RhoC full length proteins; HepG2, HeLa, Jurkat, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain and fetal kidney lysates; Mouse and rat kidney and brain lysates. IHC-P: Human stomach and squamous cell carcinoma of lung, Mouse muscle and Rat colon tissues. ICC/IF: HepG2 and NIH/3T3 cells. Flow Cyt (intra): Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18299 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/300.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
1/500.
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| WB |
1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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| IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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Flow Cyt (Intra)
1/300. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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ICC/IF
1/500. |
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WB
1/500. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
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IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Cellular localization
Rho A: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm; cytoskeleton. Rho B: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Rho C: Cell membrane; Lipid-anchor; Cytoplasmic side. -
Database links
- Entrez Gene: 387 Human
- Entrez Gene: 388 Human
- Entrez Gene: 389 Human
- Entrez Gene: 11848 Mouse
- Entrez Gene: 11852 Mouse
- Entrez Gene: 11853 Mouse
- Entrez Gene: 117273 Rat
- Entrez Gene: 295342 Rat
see all -
Alternative names
- AA017882 antibody
- AI324259, antibody
- Aplysia ras related homolog 9 (RhoC) antibody
see all
Images
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Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)All lanes : Anti-Rho A + B + C antibody [EPR18299] (ab188103) at 1/20000 dilution
Lane 1 : Human RhoA full length protein
Lane 2 : Human RhoB full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsHuman RhoA full length protein (Catalog# ab101594) contains aa1-193 with His-Tag® N-Terminus; Human RhoB full length protein (Catalog# ab107139) contains aa1-193 with His-Tag® N-Terminus.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242942-anti-rho-a-b-c-antibody-epr18299-western-blot.jpg)
Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Anti-Rho A + B + C antibody [EPR18299] (ab188103) at 1/500 dilution + Human RhoC full length protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesHuman RhoC full length protein (Catalog# ab98085) contains aa1-190 with His-Tag® N-Terminus.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242943-anti-rho-a-b-c-antibody-epr18299-western-blot.jpg)
Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)All lanes : Anti-Rho A + B + C antibody [EPR18299] (ab188103) at 1/2000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242946-anti-rho-a-b-c-antibody-epr18299-western-blot.jpg)
Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)All lanes : Anti-Rho A + B + C antibody [EPR18299] (ab188103) at 1/2000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242947-anti-rho-a-b-c-antibody-epr18299-western-blot.jpg)
Western blot - Anti-Rho A + B + C antibody [EPR18299] (ab188103)All lanes : Anti-Rho A + B + C antibody [EPR18299] (ab188103) at 1/2000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Rat brain lysate
Lane 4 : Rat kidney lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242948-anti-rho-a-b-c-antibody-epr18299-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Rho A + B + C with ab188103 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on epithelial cells of Human stomach is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242949-anti-rho-a-b-c-antibody-epr18299-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of lung tissue labeling Rho A + B + C with ab188103 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on cancer cells of squamous cell carcinoma of lung is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242950-anti-rho-a-b-c-antibody-epr18299-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Immunohistochemical analysis of paraffin-embedded Mouse muscle tissue labeling Rho A + B + C with ab188103 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on mouse muscle is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242951-anti-rho-a-b-c-antibody-epr18299-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Rho A + B + C with ab188103 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic and nuclear staining on epithelial cells of Rat colon is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242953-anti-rho-a-b-c-antibody-epr18299-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling Rho A + B + C with ab188103 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on HepG2 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab188103 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Immunocytochemistry/ Immunofluorescence - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242954-anti-rho-a-b-c-antibody-epr18299-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling to RhoA + RhoB + RhoC with ab188103 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on NIH/3T3 cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
-ve control 1: ab188103 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution. -
![Flow Cytometry (Intracellular) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-242952-anti-rho-a-b-c-antibody-epr18299-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Rho A + B + C antibody [EPR18299] (ab188103)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling Rho A + B + Cwith ab188103 at 1/300 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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![Anti-Rho A + B + C antibody [EPR18299] (ab188103)](https://www.abcam.com/ps/products/188/ab188103/Images/ab188103-13-benefits-of-recombinant-antibodies.png)
Anti-Rho A + B + C antibody [EPR18299] (ab188103)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab188103 has been referenced in 2 publications.
- Carriba P et al. CD40L Reverse Signaling Influences Dendrite Spine Morphology and Expression of PSD-95 and Rho Small GTPases. Front Cell Dev Biol 8:254 (2020). PubMed: 32411702
- Krajewski A et al. Cyclin F Downregulation Affects Epithelial-Mesenchymal Transition Increasing Proliferation and Migration of the A-375 Melanoma Cell Line. Cancer Manag Res 12:13085-13097 (2020). PubMed: 33376401