Recombinant Anti-RhoGDI antibody [EPR3773] (ab133248)
![Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)](https://www.abcam.com/ps/products/133/ab133248/Images/ab133248-481222-anti-rhogdi-antibody-epr3773-western-blot-hek293t-arhgdia-knockout-cells.jpg "Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3773] to RhoGDI
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RhoGDI antibody [EPR3773]
See all RhoGDI primary antibodies -
Description
Rabbit monoclonal [EPR3773] to RhoGDI -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, Jurkat, HeLa and NIH3T3 cell lysates. IHC-P: Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3773 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. -
Sequence similarities
Belongs to the Rho GDI family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 396 Human
- Entrez Gene: 192662 Mouse
- Entrez Gene: 360678 Rat
- Omim: 601925 Human
- SwissProt: P52565 Human
- SwissProt: Q99PT1 Mouse
- SwissProt: Q5XI73 Rat
- Unigene: 159161 Human
see all -
Alternative names
- ARHGDIA antibody
- fa96g11 antibody
- GDIA 1 antibody
see all
Images
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Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDaab133248 was shown to react with aRHGDIA in wild-type HEK293T cells in Western blot with loss of signal observed in ARHGDIA knockout cell line ab266447 (ARHGDIA knockout cell lysate ab257356). Wild-type HEK293T and ARHGDIA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133248 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2μg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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![Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)](https://www.abcam.com/ps/products/133/ab133248/Images/ab133248-395492-anti-rhogdi-antibody-epr3773-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDaLanes 1-4: Merged signal (red and green). Green - ab133248 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133248 Anti-RhoGDI antibody [EPR3773] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266446 (knockout cell lysate ab257355) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773] (ab133248)](https://www.abcam.com/ps/products/133/ab133248/Images/ab133248-146659-anti-rhogdi-antibody-epr3773-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773] (ab133248)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RhoGDI with ab133248 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)](https://www.abcam.com/ps/products/133/ab133248/Images/ab133248-393587-anti-rhogdi-antibody-epr3773-western-blot-human-knockout-lysate.jpg)
Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ARHGDIA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab133248 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133248 Anti-RhoGDI antibody [EPR3773] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266447 (knockout cell lysate ab257356) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)](https://www.abcam.com/ps/products/133/ab133248/Images/ab133248-146660-anti-rhogdi-antibody-epr3773-western-blot.jpg)
Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
![Anti-RhoGDI antibody [EPR3773] (ab133248)](https://www.abcam.com/ps/products/133/ab133248/Images/ab133248-5-benefits-of-recombinant-antibodies.png)
Anti-RhoGDI antibody [EPR3773] (ab133248)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab133248 has been referenced in 5 publications.
- Huang G et al. Downregulation of miR-483-5p inhibits TGF-ß1-induced EMT by targeting RhoGDI1 in pulmonary fibrosis. Mol Med Rep 24:N/A (2021). PubMed: 34080651
- Krafft U et al. Validation of survivin and HMGA2 as biomarkers for cisplatin resistance in bladder cancer. Urol Oncol N/A:N/A (2019). PubMed: 31053526
- Lai MC et al. Prognostic value of Rho GDP dissociation inhibitors in patients with hepatocellular carcinoma following liver transplantation. Oncol Lett 14:1395-1402 (2017). PubMed: 28789355
- Huang D et al. Rho GDP-dissociation inhibitor a is a potential prognostic biomarker and controls telomere regulation in colorectal cancer. Cancer Sci 108:1293-1302 (2017). WB ; Human . PubMed: 28417530
- Francis C et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . PubMed: 25301173