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    products/primary-antibodies/rhogdi-antibody-epr3773-ab133248.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix Structures Focal Adhesions
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-RhoGDI antibody [EPR3773] (ab133248)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (5)

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Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773] (ab133248)
  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
  • Anti-RhoGDI antibody [EPR3773] (ab133248)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3773] to RhoGDI
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human RhoGDI protein (ab132108)
Knockout
Product image
Human ARHGDIA (RhoGDI) knockout HEK-293T cell line (ab266446)
Knockout
Product image
Human ARHGDIA (RhoGDI) knockout HEK-293T cell line (ab266447)

View more associated products

Overview

  • Product name

    Anti-RhoGDI antibody [EPR3773]
    See all RhoGDI primary antibodies
  • Description

    Rabbit monoclonal [EPR3773] to RhoGDI
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, Jurkat, HeLa and NIH3T3 cell lysates. IHC-P: Human breast carcinoma tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3773
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators

Associated products

  • Alternative Versions

    • Anti-RhoGDI antibody [EPR3773] - BSA and Azide free (ab248423)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ARHGDIA (RhoGDI) knockout HEK-293T cell line (ab266446)
    • Human ARHGDIA (RhoGDI) knockout HEK-293T cell line (ab266447)
  • KO cell lysates

    • Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (ab257355)
    • Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (ab257356)
  • Positive Controls

    • Jurkat cytoplasmic extract lysate (ab14842)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human RhoGDI protein (ab132108)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them.
  • Sequence similarities

    Belongs to the Rho GDI family.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P52565 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 396 Human
    • Entrez Gene: 192662 Mouse
    • Entrez Gene: 360678 Rat
    • Omim: 601925 Human
    • SwissProt: P52565 Human
    • SwissProt: Q99PT1 Mouse
    • SwissProt: Q5XI73 Rat
    • Unigene: 159161 Human
    • Unigene: 30016 Mouse
    • Unigene: 474783 Mouse
    • Unigene: 1801 Rat
    see all
  • Alternative names

    • ARHGDIA antibody
    • fa96g11 antibody
    • GDIA 1 antibody
    • GDIA1 antibody
    • GDIR1_HUMAN antibody
    • MGC117248 antibody
    • NPHS8 antibody
    • Rho GDI 1 antibody
    • Rho GDI alpha antibody
    • Rho GDI antibody
    • Rho GDP dissociation inhibitor (GDI) alpha antibody
    • Rho GDP dissociation inhibitor 1 antibody
    • Rho GDP dissociation inhibitor alpha antibody
    • Rho GDP-dissociation inhibitor 1 antibody
    • Rho-GDI alpha antibody
    • RhoGDI 1 antibody
    • RhoGDI alpha antibody
    • RHOGDI antibody
    • RhoGDI1 antibody
    • wu:fa96g11 antibody
    • zgc:55554 antibody
    • zgc:77681 antibody
    see all

Images

  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ARHGDIA knockout HEK293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 23 kDa



    ab133248 was shown to react with aRHGDIA in wild-type HEK293T cells in Western blot with loss of signal observed in ARHGDIA knockout cell line ab266447 (ARHGDIA knockout cell lysate ab257356). Wild-type HEK293T and ARHGDIA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab133248 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2μg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ARHGDIA knockout HEK293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab133248 observed at 23 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab133248 Anti-RhoGDI antibody [EPR3773] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266446 (knockout cell lysate ab257355) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773] (ab133248)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoGDI antibody [EPR3773] (ab133248)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labelling RhoGDI with ab133248 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : ARHGDIA knockout HEK293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 23 kDa
    Observed band size: 27 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab133248 observed at 27 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab133248 Anti-RhoGDI antibody [EPR3773] was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266447 (knockout cell lysate ab257356) was used.  Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE.  ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    Western blot - Anti-RhoGDI antibody [EPR3773] (ab133248)
    All lanes : Anti-RhoGDI antibody [EPR3773] (ab133248) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : NIH3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Anti-RhoGDI antibody [EPR3773] (ab133248)
    Anti-RhoGDI antibody [EPR3773] (ab133248)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab133248? Please let us know so that we can cite the reference in this datasheet.

ab133248 has been referenced in 5 publications.

  • Huang G  et al. Downregulation of miR-483-5p inhibits TGF-ß1-induced EMT by targeting RhoGDI1 in pulmonary fibrosis. Mol Med Rep 24:N/A (2021). PubMed: 34080651
  • Krafft U  et al. Validation of survivin and HMGA2 as biomarkers for cisplatin resistance in bladder cancer. Urol Oncol N/A:N/A (2019). PubMed: 31053526
  • Lai MC  et al. Prognostic value of Rho GDP dissociation inhibitors in patients with hepatocellular carcinoma following liver transplantation. Oncol Lett 14:1395-1402 (2017). PubMed: 28789355
  • Huang D  et al. Rho GDP-dissociation inhibitor a is a potential prognostic biomarker and controls telomere regulation in colorectal cancer. Cancer Sci 108:1293-1302 (2017). WB ; Human . PubMed: 28417530
  • Francis C  et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . PubMed: 25301173

Customer reviews and Q&As

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Western blot abreview for Anti-RhoGDI antibody [EPR3773]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Jun 22 2017

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