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Recombinant

Recombinant Anti-RIP Antibody [RP23040227] (ab313489)

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Western blot - Anti-RIP Antibody [RP23040227] (ab313489)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIP Antibody [RP23040227] (ab313489)
  • Immunocytochemistry/ Immunofluorescence - Anti-RIP Antibody [RP23040227] (ab313489)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit recombinant multiclonal [RP23040227] to RIP
  • Suitable for: ICC/IF, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-RIP Antibody [RP23040227]
    See all RIP primary antibodies

  • Description

    Rabbit recombinant multiclonal [RP23040227] to RIP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human RIP aa 1-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q13546

  • Positive control

    • ICC/IF: COLO-205 cells, HT29 cells WB: Jurkat cell lysate, COLO205 cell lysate, SW480 cell lysate, HT29 cell lysate, HCT116 cell lysate, MDA-MB-231 cell lysate, MCF7 cell lysate, HepG2 cell lysate, A549 cell lysate, HeLa cell lysate

  • General notes

    Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

    Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab313489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 2.5 µg/ml. Predicted molecular weight: 76 kDa.
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 2.5 µg/ml. Predicted molecular weight: 76 kDa.

Target

  • Function

    Essential adapter molecule for the activation of NF-kappa-B. Following different upstream signals (binding of inflammatory cytokines, stimulation of pathogen recognition receptors, or DNA damage), particular RIPK1-containing complexes are formed, initiating a limited number of cellular responses. Upon TNFA stimulation RIPK1 is recruited to a TRADD-TRAF complex initiated by TNFR1 trimerization. There, it is ubiquitinated via 'Lys-63'-link chains, inducing its association with the IKK complex, and its activation through NEMO binding of polyubiquitin chains.

  • Sequence similarities

    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 death domain.
    Contains 1 protein kinase domain.

  • Post-translational
    modifications

    Proteolytically cleaved by caspase-8 during TNF-induced apoptosis. Cleavage abolishes NF-kappa-B activation and enhances pro-apototic signaling through the TRADD-FADD interaction.
    Autophosphorylated on serine and threonine residues.
    Ubiquitinated by 'Lys-11'-, 'Lys-48'-, 'Lys-63'- and linear-linked type ubiquitin. Polyubiquitination with 'Lys-63'-linked chains by TRAF2 induces association with the IKK complex. Deubiquitination of 'Lys-63'-linked chains and polyubiquitination with 'Lys-48'-linked chains by TNFAIP3 leads to RIPK1 proteasomal degradation and consequently to the termination of the TNF- or Linear polyubiquitinated; the head-to-tail polyubiquitination is mediated by the LUBAC complex. LPS-mediated activation of NF-kappa-B. Also ubiquitinated with 'Lys-11'-linked chains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell death protein RIP antibody
      • FLJ39204 antibody
      • OTTHUMP00000039163 antibody
      • Receptor (TNFRSF) interacting serine threonine kinase 1 antibody
      • receptor interacting protein 1 antibody
      • Receptor interacting protein antibody
      • Receptor interacting protein kinase 1 antibody
      • Receptor interacting serine threonine protein kinase 1 antibody
      • Receptor TNFRSF interacting serine threonine kinase 1 antibody
      • Receptor-interacting protein 1 antibody
      • Receptor-interacting serine/threonine-protein kinase 1 antibody
      • Rinp antibody
      • RIP 1 antibody
      • RIP antibody
      • Rip-1 antibody
      • RIP1 antibody
      • RIPK 1 antibody
      • Ripk1 antibody
      • RIPK1_HUMAN antibody
      • Serine threonine protein kinase RIP antibody
      • Serine/threonine-protein kinase RIP antibody
      see all

    Images

    • Western blot - Anti-RIP Antibody [RP23040227] (ab313489)
      Western blot - Anti-RIP Antibody [RP23040227] (ab313489)
      All lanes : Anti-RIP Antibody [RP23040227] (ab313489) at 2.5 µg/ml

      Lane 1 : Jurkat cell lysate
      Lane 2 : COLO205 cell lysate
      Lane 3 : SW480 cell lysate
      Lane 4 : HT29 cell lysate
      Lane 5 : HCT116 cell lysate
      Lane 6 : MDA-MB-231 cell lysate
      Lane 7 : MCF7 cell lysate
      Lane 8 : HepG2 cell lysate
      Lane 9 : A549 cell lysate
      Lane 10 : HeLa cell lysate

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

      Predicted band size: 76 kDa
      Observed band size: 76 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RIP Antibody [RP23040227] (ab313489)
      Immunocytochemistry/ Immunofluorescence - Anti-RIP Antibody [RP23040227] (ab313489)
      Immunofluorescent analysis of COLO-205 cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling endogenous RIPK1 with ab313489 at 5 µg/mL dilution followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of RIPK1 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of RIPK1. Panel represents control cells with no primary antibody to assess background. Panel f) represents SW480 cells which is negative for RIPK1 showing no signal.
    • Immunocytochemistry/ Immunofluorescence - Anti-RIP Antibody [RP23040227] (ab313489)
      Immunocytochemistry/ Immunofluorescence - Anti-RIP Antibody [RP23040227] (ab313489)
      Immunofluorescent analysis of HT29 cells transfected with RIPK1 specific siRNA fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling RIPK1 with ab313489 at 5 µg/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a-d) untransfected HT29 cells. Panel b, e) HT29 cells transfected with non-specific scrambled siRNA. Panel c,f) HT29 cells transfected with RIPK1 specific siRNA. Nuclei (blue) were stained using DAPI, and Rhodamine Phalloidin at 1/300 dilution. was used for cytoskeletal F-actin (red) staining. Loss of signal was observed upon siRNA mediated knockdown (panel c,f) confirming specificity of the antibody to RIPK1 (green). The images were captured at 60X magnification

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab313489? Please let us know so that we can cite the reference in this datasheet.

    ab313489 has not yet been referenced specifically in any publications.

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