Recombinant Anti-RNA polymerase beta antibody [EPR18704] (ab191598)
![Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](https://www.abcam.com/ps/products/191/ab191598/Images/ab191598-246642-anti-rna-polymerase-beta-antibody-epr18704-western-blot.jpg "Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18704] to RNA polymerase beta
- Suitable for: IP, WB
- Reacts with: Escherichia coli
Related conjugates and formulations
Overview
-
Product name
Anti-RNA polymerase beta antibody [EPR18704]
See all RNA polymerase beta primary antibodies -
Description
Rabbit monoclonal [EPR18704] to RNA polymerase beta -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Escherichia coli -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB & IP: E.coli whole cell lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18704 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191598 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/30.
|
|
| WB | (2) |
1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
|
| Notes |
|---|
|
IP
1/30. |
|
WB
1/2000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
Target
-
Relevance
RNA polymerase beta (rpoB) is the ß subunit of the bacterial RNA polymerase and is the subunit which possesses the polymerase activity. This protein is a highly conserved enzyme contained by many bacteria and has been reported as a useful marker for studies in bacetrial identification and ecology. -
Cellular localization
Cell Membrane and Cytoplasmic -
Database links
- Entrez Gene: 948488 Escherichia coli
- SwissProt: P0A8V2 Escherichia coli
-
Alternative names
- groN antibody
- nitB antibody
- rif antibody
see all
Images
-
Western blot - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)Anti-RNA polymerase beta antibody [EPR18704] (ab191598) at 1/2000 dilution + E.coli whole cell lysates at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
-
![Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](https://www.abcam.com/ps/products/191/ab191598/Images/ab191598-246641-anti-rna-polymerase-beta-antibody-epr18704-immunoprecipitation.jpg)
Immunoprecipitation - Anti-RNA polymerase beta antibody [EPR18704] (ab191598)RNA polymerase beta was immunoprecipitated from 1mg of E.coli whole cell lysates with ab191598 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191598 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.
Lane 1: E.coli whole cell lysates 10ug (Input). Lane 2: ab191598 IP in E.coli whole cell lysates. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191598 in E.coli whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
-
![Anti-RNA polymerase beta antibody [EPR18704] (ab191598)](https://www.abcam.com/ps/products/191/ab191598/Images/ab191598-3-benefits-of-recombinant-antibodies.png)
Anti-RNA polymerase beta antibody [EPR18704] (ab191598)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (7)
ab191598 has been referenced in 7 publications.
- Plaza N et al. Identification of a Family of Vibrio Type III Secretion System Effectors That Contain a Conserved Serine/Threonine Kinase Domain. mSphere 6:e0059921 (2021). PubMed: 34346702
- Zhang X et al. Bis-molybdopterin guanine dinucleotide modulates hemolysin expression under anaerobiosis and contributes to fitness in vivo in uropathogenic Escherichia coli. Mol Microbiol 116:1216-1231 (2021). PubMed: 34494331
- Liao J et al. Bacterial quorum sensing quenching activity of Lysobacter leucyl aminopeptidase acts by interacting with autoinducer synthase. Comput Struct Biotechnol J 19:6179-6190 (2021). PubMed: 34900131
- Stårsta M et al. RHS-elements function as type II toxin-antitoxin modules that regulate intra-macrophage replication of Salmonella Typhimurium. PLoS Genet 16:e1008607 (2020). PubMed: 32053596
- Klein K et al. Deprivation of the Periplasmic Chaperone SurA Reduces Virulence and Restores Antibiotic Susceptibility of Multidrug-Resistant Pseudomonas aeruginosa. Front Microbiol 10:100 (2019). PubMed: 30846971
- Virtanen P et al. Class II contact-dependent growth inhibition (CDI) systems allow for broad-range cross-species toxin delivery within the Enterobacteriaceae family. Mol Microbiol 111:1109-1125 (2019). PubMed: 30710431
- Høyland-Kroghsbo NM et al. Temperature, by Controlling Growth Rate, Regulates CRISPR-Cas Activity in Pseudomonas aeruginosa. MBio 9:N/A (2018). PubMed: 30425154