Anti-ROCK1 (phospho T455 + S456) antibody (ab203273)
Key features and details
- Rabbit polyclonal to ROCK1 (phospho T455 + S456)
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ROCK1 (phospho T455 + S456) antibody
See all ROCK1 primary antibodies -
Description
Rabbit polyclonal to ROCK1 (phospho T455 + S456) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human ROCK1 aa 400-500 (phospho T455 + S456) conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13464 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.01% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200 |
| Notes |
|---|
|
IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200 |
Target
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Function
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. -
Tissue specificity
Detected in blood platelets. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 REM (Hr1) repeat. -
Domain
The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated. -
Post-translational
modificationsAutophosphorylated on serine and threonine residues.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing. -
Cellular localization
Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Golgi apparatus membrane. Cell projection, bleb. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, ruffle. Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes. - Information by UniProt
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Database links
- Entrez Gene: 6093 Human
- Entrez Gene: 81762 Rat
- Omim: 601702 Human
- SwissProt: Q13464 Human
- SwissProt: Q63644 Rat
- Unigene: 306307 Human
- Unigene: 89756 Rat
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Alternative names
- coiled-coil-containing protein kinase 1 antibody
- coiled-coil-containing protein kinase I antibody
- MGC131603 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 (phospho T455 + S456) antibody (ab203273)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded rat heart tissue labeling ROCK1 with ab203273 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 (phospho T455 + S456) antibody (ab203273)Immunohistochemical analysis of formalin-fixed and paraffin-embedded human lung carcinoma tissue labeling ROCK1 with ab203273 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab203273 has been referenced in 9 publications.
- Luo S et al. Activation of TMEM16A Ca2+-activated Cl- channels by ROCK1/moesin promotes breast cancer metastasis. J Adv Res 33:253-264 (2021). PubMed: 34603794
- Zhao Y et al. Microbial recognition by GEF-H1 controls IKKe mediated activation of IRF5. Nat Commun 10:1349 (2019). PubMed: 30902986
- Ma X et al. Analyze and Identify Peiminine Target EGFR Improve Lung Function and Alleviate Pulmonary Fibrosis to Prevent Exacerbation of Chronic Obstructive Pulmonary Disease by Phosphoproteomics Analysis. Front Pharmacol 10:737 (2019). PubMed: 31333459
- Wang W et al. SMAD specific E3 ubiquitin protein ligase 1 promotes ovarian cancer cell migration and invasion via the activation of the RhoA/ROCK signaling pathway. Oncol Rep 41:668-676 (2019). PubMed: 30542716
- Yang L et al. Anti-gastric cancer effect of Salidroside through elevating miR-99a expression. Artif Cells Nanomed Biotechnol 47:3500-3510 (2019). PubMed: 31432697
- Wong WY et al. Persistent disruption of lateral junctional complexes and actin cytoskeleton in parotid salivary glands following radiation treatment. Am J Physiol Regul Integr Comp Physiol 315:R656-R667 (2018). PubMed: 29897817
- Wang J et al. Rho A Regulates Epidermal Growth Factor-Induced Human Osteosarcoma MG63 Cell Migration. Int J Mol Sci 19:N/A (2018). PubMed: 29751634
- Fu J et al. 1, 6-di-O-caffeoyl-ß-D-glucopyranoside, a natural compound from Callicarpa nudiflora Hook impairs P2Y12 and thromboxane A2 receptor-mediated amplification of platelet activation and aggregation. Phytomedicine 36:273-282 (2017). PubMed: 29157825
- Zhu KC et al. Afzelin exhibits anti-cancer activity against androgen-sensitive LNCaP and androgen-independent PC-3 prostate cancer cells through the inhibition of LIM domain kinase 1. Oncol Lett 10:2359-2365 (2015). WB ; Human . PubMed: 26622852