Recombinant Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)
![Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.com/ps/products/187/ab187657/Images/ab187657-220694-anti-ror-beta-antibody-epr15552-n-terminal-western-blot.jpg "Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15552] to ROR beta/RORB - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ROR beta/RORB antibody [EPR15552] - N-terminal
See all ROR beta/RORB primary antibodies -
Description
Rabbit monoclonal [EPR15552] to ROR beta/RORB - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 whole cell lysate (ab7900) and human cerebellum tissue lysate; ICC/IF: Mouse primary neural/glia cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15552 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Predicted molecular weight: 53 kDa.
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| ICC/IF |
1/1000.
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| Notes |
|---|
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WB
1/1000 - 1/2000. Predicted molecular weight: 53 kDa. |
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ICC/IF
1/1000. |
Target
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Function
Orphan nuclear receptor required for normal postnatal development of rod and cone photoreceptor cells. Regulates transcription of OPN1SW in cone photoreceptor cells by binding the sequence 5'-AGGTCA-3' in the OPN1SW promoter. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6096 Human
- Entrez Gene: 225998 Mouse
- Omim: 601972 Human
- SwissProt: Q92753 Human
- SwissProt: Q8R1B8 Mouse
- Unigene: 494178 Human
- Unigene: 234641 Mouse
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Alternative names
- bA133M9.1 antibody
- NR1F2 antibody
- Nuclear receptor ROR-beta antibody
see all
Images
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Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.com/ps/products/187/ab187657/Images/ab187657-4-anti-ror-betarorb-antibody-epr15552-n-terminal-immunocytochemistry-mouse-primary-neuralglia-cell1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cells labelling ROR beta/RORB with ab187657 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplamic staining in astrocytes. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. Anti-Glial Fibrillary Acidic Protein (GFAP) mouse monoclonal antibody was used to counterstain tubulin at 1/100 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).
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![Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.com/ps/products/187/ab187657/Images/ab187657-3-anti-ror-betarorb-antibody-epr15552-n-terminal-immunocytochemistry-mouse-primary-neuralglia-cell.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural / glia cells labelling ROR beta/RORB with ab187657 at 1/1000 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing cytoplasmic staining in mouse primary astrocyte. Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 dilution (4 µg/mL) (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution (2 µg/mL).
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![Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.com/ps/products/187/ab187657/Images/ab187657-220695-anti-ror-beta-antibody-epr15552-n-terminal-western-blot.jpg)
Western blot - Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657) at 1/1000 dilution + Human cerebellum tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa -
![Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)](https://www.abcam.com/ps/products/187/ab187657/Images/ab187657-5-benefits-of-recombinant-antibodies.png)
Anti-ROR beta/RORB antibody [EPR15552] - N-terminal (ab187657)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab187657 has not yet been referenced specifically in any publications.