Anti-ROS1 antibody [4-6G] (ab108492)
Key features and details
- Mouse monoclonal [4-6G] to ROS1
- Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-ROS1 antibody [4-6G]
See all ROS1 primary antibodies -
Description
Mouse monoclonal [4-6G] to ROS1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-Fr, IHC-P, IP, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to ROS1. Extracellular portion of ROS amino acid 1-285 fused to Fc, transiently expressed in 293 cells and purified using PtnA column chromatography.
-
Positive control
- Cells transiently expressing human ROS1, mouse cells expressing human ROS1.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
4-6G -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (3) |
Use at an assay dependent concentration.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| WB |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
ICC/IF
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB
Use at an assay dependent concentration. |
Target
-
Function
This is probably a cell growth or differentiation factor receptor with a tyrosine-protein kinase activity. -
Tissue specificity
Expressed in brain. Expression is increased in primary gliomas. -
Involvement in disease
Note=A chromosomal aberration involving ROS1 is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 9 fibronectin type-III domains.
Contains 1 protein kinase domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 6098 Human
- Omim: 165020 Human
- SwissProt: P08922 Human
- Unigene: 1041 Human
-
Alternative names
- c ros 1 antibody
- c ros antibody
- c ros oncogene 1 receptor tyrosine kinase antibody
see all
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab108492 has been referenced in 4 publications.
- Zhang X et al. The CtBP1-HDAC1/2-IRF1 transcriptional complex represses the expression of the long noncoding RNA GAS5 in human osteosarcoma cells. Int J Biol Sci 15:1460-1471 (2019). PubMed: 31337976
- Fei J et al. Low concentration of rutin treatment might alleviate the cardiotoxicity effect of pirarubicin on cardiomyocytes via activation of PI3K/AKT/mTOR signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31138757
- Shih CH et al. EZH2-mediated upregulation of ROS1 oncogene promotes oral cancer metastasis. Oncogene 36:6542-6554 (2017). PubMed: 28759046
- Deng G et al. Downregulation of ROS-FIG inhibits cell proliferation, colony-formation, cell cycle progression, migration and invasion, while inducing apoptosis in intrahepatic cholangiocarcinoma cells. Int J Mol Med 34:661-8 (2014). WB, IHC-P ; Human . PubMed: 24968753