Recombinant Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)
![Immunoprecipitation - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)](https://www.abcam.com/ps/products/264/ab264077/Images/ab264077-5-anti-runx2-antibody-epr22858-106-immunoprecipitation-saos2-human.jpg "Immunoprecipitation - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22858-106] to RUNX2 - BSA and Azide free
- Suitable for: WB, IP, IHC-P, ChIP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free
See all RUNX2 primary antibodies -
Description
Rabbit monoclonal [EPR22858-106] to RUNX2 - BSA and Azide free -
Host species
Rabbit -
Specificity
ab264077 is not recommended for mouse IHC.
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Tested applications
Suitable for: WB, IP, IHC-P, ChIPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC-3, MDA-MB-231, Saos-2, MEF, C2C12, MCF7, HeLa, C6 and PC-12 lysates. IHC-P: Human osteosarcoma and Rat embryo 14.5 day tissues. IP: PC-3 and Saos-2 cells.
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General notes
ab264077 is the carrier-free version of ab236639.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22858-106 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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| IP |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| ChIP |
Use 5 µg for 25 µg of chromatin.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
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IP
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ChIP
Use 5 µg for 25 µg of chromatin. |
Target
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Function
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. -
Tissue specificity
Specifically expressed in osteoblasts. -
Involvement in disease
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. -
Sequence similarities
Contains 1 Runt domain. -
Domain
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. -
Post-translational
modificationsPhosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 860 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 367218 Rat
- Omim: 600211 Human
- SwissProt: Q13950 Human
- SwissProt: Q08775 Mouse
- SwissProt: Q9Z2J9 Rat
- Unigene: 535845 Human
see all -
Alternative names
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
- AML3 antibody
see all
Images
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Immunoprecipitation - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)
RUNX2 was immunoprecipitated from 0.35 mg Saos-2 (human osteosarcoma epithelial) whole cell lysate with ab236639 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236639 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Saos-2 (human osteosarcoma epithelial) whole cell lysate 10ug
Lane 2: ab236639 IP in Saos-2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236639 in Saos-2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236639).
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![Immunoprecipitation - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)](https://www.abcam.com/ps/products/264/ab264077/Images/ab264077-4-anti-runx2-antibody-epr22858-106-immunoprecipitation-pc3-human.jpg)
Immunoprecipitation - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)RUNX2 was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 10ug with ab236639 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236639 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2: ab236639 IP in PC-3 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236639 in PC-3 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236639).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)](https://www.abcam.com/ps/products/264/ab264077/Images/ab264077-3-anti-runx2-antibody-epr22858-106-immunohistochemistry-osteosarcoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue labeling RUNX2 with ab236639 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human osteosarcoma (PMID: 21731849) is observed. The section was incubated with ab236639 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236639).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)](https://www.abcam.com/ps/products/264/ab264077/Images/ab264077-2-anti-runx2-antibody-epr22858-106-immunohistochemistry-embryo-145-day-rat.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)Immunohistochemical analysis of paraffin-embedded rat embryo 14.5 day tissue labeling RUNX2 with ab236639 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the cartilage cells in the rat embryo 14.5 day tissue is observed. The section was incubated with ab236639 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236639).
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![ChIP - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)](https://www.abcam.com/ps/products/264/ab264077/Images/ab264077-1-anti-runx2-antibody-epr22858-106-chromatin-immunoprecipitation-saos2-human.jpg)
ChIP - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)Chromatin was prepared from Saos-2 cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 µg of chromatin, 5 µg of ab236639 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab236639).
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![Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)](https://www.abcam.com/ps/products/264/ab264077/Images/ab264077-6-benefits-of-recombinant-antibodies.png)
Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (ab264077)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab264077 has not yet been referenced specifically in any publications.
