Recombinant Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR26288-70] to Ryanodine receptor 2/RYR-2
- Suitable for: IHC-P, IHC-Fr, IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70]
See all Ryanodine receptor 2/RYR-2 primary antibodies -
Description
Rabbit monoclonal [EPR26288-70] to Ryanodine receptor 2/RYR-2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Tissue lysates: Mouse and rat heart; HEK-293T transfected with human Ryanodine receptor 2 expression vector with a myc-His-tag®. IHC-P: Human: cardiac muscle, cerebrum tissues; Mouse and rat cardiac muscle tissues. IHC-Fr: Mouse and rat frozen heart (fresh). IP: Mouse heart tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR26288-70 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab302716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/100.
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 564 kDa (predicted molecular weight: 565 kDa).
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. |
IP
1/30. |
WB
1/1000. Detects a band of approximately 564 kDa (predicted molecular weight: 565 kDa). |
Target
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Function
Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development. -
Tissue specificity
Detected in heart muscle (at protein level). Heart muscle, brain (cerebellum and hippocampus) and placenta. -
Involvement in disease
Familial arrhythmogenic right ventricular dysplasia 2
Ventricular tachycardia, catecholaminergic polymorphic 1, with or without atrial dysfunction and/or dilated cardiomyopathy -
Sequence similarities
Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR2 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains. -
Developmental stage
Expressed in myometrium during pregnancy. -
Domain
The calcium release channel activity resides in the C-terminal region while the remaining part of the protein resides in the cytoplasm. -
Post-translational
modificationsChannel activity is modulated by phosphorylation. Phosphorylation at Ser-2808 and Ser-2814 increases the open probability of the calcium channel. Phosphorylation is increased in failing heart, leading to calcium leaks and increased cytoplasmic Ca(2+) levels.
Phosphorylation at Ser-2031 by PKA enhances the response to lumenal calcium. -
Cellular localization
Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 6262 Human
- Entrez Gene: 20191 Mouse
- Entrez Gene: 689560 Rat
- Omim: 180902 Human
- SwissProt: Q92736 Human
- SwissProt: E9Q401 Mouse
- SwissProt: B0LPN4 Rat
- Unigene: 109514 Human
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Alternative names
- ARVC2 antibody
- ARVD2 antibody
- Cardiac muscle ryanodine receptor antibody
see all
Images
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All lanes : Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat heart tissue lysate
Lane 5 : Rat skeletal muscle tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 565 kDa
Observed band size: 564 kDa why is the actual band size different from the predicted?Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Negative controls: skeletal muscle, liver (PMID:23516528, PMID: 9607712)
Exposure time: Lanes 1-3: 15 seconds; Lanes 4-6: 37 seconds.
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All lanes : Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (ab302716) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with human Ryanodine receptor 2 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 565 kDa
Observed band size: 564 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 dilution (1.068 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cardiac muscle is observed (PMID: 27987400). The section was incubated with ab302716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 dilution (1.068 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human cerebrum is observed. The section was incubated with ab302716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/2000 dilution (0.267 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on mouse cardiac muscle is observed.The section was incubated with ab302716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/2000 dilution (0.267 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on rat cardiac muscle (PMID: 27882143).The section was incubated with ab302716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 dilution (1.068 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) was used. Negative control: no staining on human liver is observed. The section was incubated with ab302716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Ryanodine receptor 2/RYR-2 with ab302716 at 1/500 dilution (1.068 µg/ml), followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection). Negative control: no staining on human skeletal muscle is observed. The section was incubated with ab302716 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with AB302716 at 1/100 dilution (5.34 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on mouse heart is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with AB302716 at 1/100 dilution (5.34 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on mouse liver is observed (PMID: 12395283). The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with AB302716 at 1/100 dilution (5.34 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Positive staining on rat heart is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Ryanodine receptor 2/RYR-2 with AB302716 at 1/100 dilution (5.34 µg/mL), followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Negative control: no staining on rat liver (PMID:�12395283) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 dilution (2 µg/mL).
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Ryanodine receptor 2/RYR-2 was immunoprecipitated from 0.35 mg mouse heart tissue lysate with ab302716 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302716 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: mouse heart tissue lysate 10 µg (Inset)
Lane 2: ab302716 IP in mouse heart tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab302716 in mouse heart tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Observed MW (kDa): 564.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab302716 has not yet been referenced specifically in any publications.