Recombinant Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Product name

Anti-Ryanodine Receptor antibody [EPR21796]
See all Ryanodine Receptor primary antibodies
Description

Rabbit monoclonal [EPR21796] to Ryanodine Receptor
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human skeletal muscle tissue lysate; mouse skeletal muscle tissue lysate; rat skeletal muscle tissue lysate. IHC-P: Mouse skeletal muscle and cerebellum tissue; rat skeletal muscle and cerebellum tissue. IHC-Fr: Mouse skeletal muscle and cerebellum tissue; rat skeletal muscle and cerebellum tissue. ICC/IF: C2C12 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21796
Isotype

IgG
Research areas
- Neuroscience
- Processes

Alternative Versions
- Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab219798 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/100.
WB		1/1000. Detects a band of approximately 565 kDa (predicted molecular weight: 565 kDa).
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is not recommended in human due to non-specific staining.
IHC-Fr		1/30. Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0). IHC is not recommended in human due to non-specific staining.

Notes
ICC/IF 1/100.
WB 1/1000. Detects a band of approximately 565 kDa (predicted molecular weight: 565 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. IHC is not recommended in human due to non-specific staining.
IHC-Fr 1/30. Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0). IHC is not recommended in human due to non-specific staining.

Function

Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
Tissue specificity

Skeletal muscle and brain (cerebellum and hippocampus).
Involvement in disease

Malignant hyperthermia 1
Central core disease of muscle
Multiminicore disease with external ophthalmoplegia
Myopathy, congenital, with fiber-type disproportion
Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood.
Sequence similarities

Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains.
Domain

The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule.
Post-translational
modifications

Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation.
Cellular localization

Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
Target information above from: UniProt accession P21817 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6261 Human
- Entrez Gene: 6262 Human
- Entrez Gene: 6263 Human
- Entrez Gene: 20190 Mouse
- Entrez Gene: 20191 Mouse
- Entrez Gene: 20192 Mouse
- Entrez Gene: 114207 Rat
- Entrez Gene: 170546 Rat
- Entrez Gene: 689560 Rat
- Omim: 180901 Human
- Omim: 180902 Human
- Omim: 180903 Human
- SwissProt: P21817 Human
- SwissProt: Q15413 Human
- SwissProt: Q92736 Human
- SwissProt: E9PZQ0 Mouse
- SwissProt: Q14AQ9 Mouse
- SwissProt: Q80X16 Mouse
- SwissProt: Q80ZZ5 Mouse
- SwissProt: F1LMY4 Rat
- Unigene: 466664 Human
- Unigene: 439745 Mouse
- Unigene: 225306 Rat
- Unigene: 87520 Rat
see all
Alternative names
- Arrhythmogenic right ventricular dysplasia 2 antibody
- ARVC2 antibody
- ARVD2 antibody
- Brain ryanodine receptor calcium release channel antibody
- Brain type ryanodine receptor antibody
- Cardiac muscle ryanodine receptor calcium release channel antibody
- Cardiac muscle type ryanodine receptor antibody
- CCO antibody
- Central core disease of muscle antibody
- HBRR antibody
- hRYR 2 antibody
- hRYR2 antibody
- MHS antibody
- MHS1 antibody
- Ryanodine receptor 1 (skeletal) antibody
- Ryanodine receptor 1 antibody
- Ryanodine receptor 2 (cardiac) antibody
- Ryanodine receptor 2 antibody
- Ryanodine receptor 3 antibody
- Ryanodine receptor type1 antibody
- RYDR antibody
- RYR 1 antibody
- RYR 2 antibody
- RYR 3 antibody
- RYR antibody
- RYR-1 antibody
- RyR1 antibody
- RYR1_HUMAN antibody
- RYR2 antibody
- RYR3 antibody
- Sarcoplasmic reticulum calcium release channel antibody
- Skeletal muscle calcium release channel antibody
- Skeletal muscle ryanodine receptor antibody
- Skeletal muscle type ryanodine receptor antibody
- Skeletal muscle-type ryanodine receptor antibody
- SKRR antibody
- Type 1 like ryanodine receptor antibody
- Type 1 ryanodine receptor antibody
- VTSIP antibody
see all

Western blot - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

All lanes : Anti-Ryanodine Receptor antibody [EPR21796] (ab219798) at 1/1000 dilution

Lane 1 : Human skeletal muscle tissue lysate at 20 µg
Lane 2 : Mouse skeletal muscle tissue lysate at 20 µg
Lane 3 : Rat skeletal muscle tissue lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 565 kDa
Observed band size: 565 kDa

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lanes 1 & 3: 3 minutes.

Lane 2: 15 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of C2C12 (mouse muscle myoblast) cells labeling Ryanodine Receptor (green) with ab219798 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Confocal image showing cytoplasmic staining in differentiated C2C12 cells. Confluent C2C12 cells were grown for 8 days to differentiate into myotube in a complete culture medium containing 10% FBS, following the ATCC protocol for myotube formation. The nuclear counter stain is DAPI (blue).

ab195889, Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 was used as counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of mouse cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse skeletal muscle tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. Positive staining in mouse skeletal muscle cells (PMID: 21454501) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of rat cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in rat skeletal muscle (PMID: 26109061) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse skeletal muscle (PMID: 26109061) is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. Positive cytoplasmic staining in Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of rat cerebellum tissue section (PMID: 18313230) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).
Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat skeletal muscle tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. Positive staining of rat skeletal muscle cells (PMID: 21454501) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).
Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution. Positive cytoplasmic staining in the Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of mouse cerebellum tissue section (PMID: 18313230) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).
Anti-Ryanodine Receptor antibody [EPR21796] (ab219798)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab219798? Please let us know so that we can cite the reference in this datasheet.

ab219798 has been referenced in 2 publications.

Camarero-Espinosa S & Moroni L Janus 3D printed dynamic scaffolds for nanovibration-driven bone regeneration. Nat Commun 12:1031 (2021). PubMed: 33589620
Guo W et al. The ryanodine receptor stabilizer S107 ameliorates contractility of adult Rbm20 knockout rat cardiomyocytes. Physiol Rep 9:e15011 (2021). PubMed: 34523260

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (2)

Customer reviews and Q&As

Post-translational
modifications