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RecombinantRabMAb

Recombinant Anti-S100 alpha antibody [EPR19013] (ab183979)

Submit a review Submit a question References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Flow Cytometry (Intracellular) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (ab183979)
  • Anti-S100 alpha antibody [EPR19013] (ab183979)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19013] to S100 alpha
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-S100 alpha antibody [EPR19013]
    See all S100 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR19013] to S100 alpha

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skeletal muscle lysate; Mouse cerebral cortex, liver, heart and muscle lysates; RAW 264.7 whole cell lysate. IHC-P: Human cerebrum, tonsil and kidney tissues. IHC-Fr: Mouse brain, kidney and spleen tissues. IP: RAW 264.7 whole cell lysate. Flow Cyt (intra): C2C12

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP
1/40.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Weak positive staining was observed in mouse with minimal background.
Notes
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
IP
1/40.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Weak positive staining was observed in mouse with minimal background.

Target

  • Function

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

  • Tissue specificity

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 Alpha Chain antibody
      • S100 antibody
      • S100 Beta Chain antibody
      • S100 Calcium Binding Protein A1 antibody
      • S100 Calcium Binding Protein B antibody
      • S100 Calcium Binding Protein Beta Neural antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S10A1_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive on germinal center of Human tonsil. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Flow Cytometry (Intracellular) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      C2C12 (mouse muscle) cells were fixed with 4% paraformaldehyde and incubated with the primary antibody (ab183979; red line) diluted 1/60 (1µg). The secondary antibody used was Goat anti-rabbit IgG (Alexa Fluor®488) at 1/500 dilution. The isotype control (black line) antibody wasRabbit monoclonal IgG (ab172730). An unlabelled control without incubation with primary antibody and secondary antibody was also perfomed (blue line). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on glial cells and negative on neuron cells of Human cerebrum was observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Part of kidney tubules were strongly staining. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain tissue labeling S100 with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on glial cells of mouse cortex. The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling S100 with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse kidney. The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (ab183979)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling S100 with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse spleen. The nuclear counterstain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

    • Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
      All lanes : Anti-S100 alpha antibody [EPR19013] (ab183979) at 1/2000 dilution

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Mouse cerebral cortex lysate
      Lane 3 : Mouse liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 11 kDa
      Observed band size: 11 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Note that high molecular weight bands were detected in mouse tissue lysates.

    • Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Anti-S100 alpha antibody [EPR19013] (ab183979) at 1/2000 dilution + RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 11 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979)
      All lanes : Anti-S100 alpha antibody [EPR19013] (ab183979) at 1/1000 dilution

      Lane 1 : Mouse muscle lysate
      Lane 2 : Mouse heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 11 kDa
      Observed band size: 11 kDa


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      Note that high molecular weight bands were detected in mouse tissue lysates.

    • Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (ab183979)
      Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (ab183979)

      S100 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab183979 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183979 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: RAW 264.7 whole cell lysate, 10µg (Input).

      Lane 2: ab183979 IP in RAW 264.7 whole cell lysate.

      Lane 3: Rabbit IgG, monoclonal [EPR25A]  - Isotype Control (ab172730) instead of ab183979 in RAW 264.7 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

    • Anti-S100 alpha antibody [EPR19013] (ab183979)
      Anti-S100 alpha antibody [EPR19013] (ab183979)

    References (5)

    Publishing research using ab183979? Please let us know so that we can cite the reference in this datasheet.

    ab183979 has been referenced in 5 publications.

    • Liu JL  et al. Increased nuclear translation of YAP might act as a potential therapeutic target for NF1-related plexiform neurofibroma. Int J Med Sci 18:2008-2016 (2021). PubMed: 33850471
    • Kanazawa K  et al. Ppp6c haploinsufficiency accelerates UV-induced BRAF(V600E)-initiated melanomagenesis. Cancer Sci 112:2233-2244 (2021). PubMed: 33743547
    • Wang Q  et al. Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas. Sci Rep 11:1314 (2021). PubMed: 33446752
    • Zhang Y  et al. Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration. Neural Regen Res 15:1903-1911 (2020). PubMed: 32246639
    • Gonçalves AF  et al. Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells. Nat Commun 8:1466 (2017). IHC-P . PubMed: 29133867

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