Recombinant Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
![Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-391288-anti-s100a12cgrpantibody-epr23677111-western-blot-human-spleen-tissue-lysate-40-ug.jpg "Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23677-111] to S100A12/CGRP
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-S100A12/CGRP antibody [EPR23677-111]
See all S100A12/CGRP primary antibodies -
Description
Rabbit monoclonal [EPR23677-111] to S100A12/CGRP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human spleen and lymph node tissue lysate. IHC-P: Human spleen tissue. Human bladder cancer and cervical cancer tissue. ICC/IF: Human PBMCs. Flow Cyt (intra): Human PBMCs. IP: Human lymph node tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23677-111 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/400.
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| WB |
1/1000. Predicted molecular weight: 10 kDa.
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| IHC-P |
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| IP |
1/30.
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| ICC/IF |
1/100.
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| Notes |
|---|
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Flow Cyt (Intra)
1/400. |
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WB
1/1000. Predicted molecular weight: 10 kDa. |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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IP
1/30. |
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ICC/IF
1/100. |
Target
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Function
Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Binds calcium, zinc and copper. Presence of zinc increases the affinity for calcium. Plays an important role in the inflammatory response. Interaction with AGER on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. -
Tissue specificity
Monocytes and lymphocytes. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 6283 Human
- Omim: 603112 Human
- SwissProt: P80511 Human
- Unigene: 19413 Human
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Alternative names
- CAAF1 antibody
- CAGC antibody
- Calcitermin antibody
see all
Images
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Western blot - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)All lanes : Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713) at 1/1000 dilution
Lane 1 : Human spleen tissue lysate
Lane 2 : Human lymph node tissue lysate
Lane 3 : Human brain tissue lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 19542470).
Negative control: Human brain (PMID: 8718672).
Exposure time: 103 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-1-anti-s100a12cgrpantibody-epr23677111-immunohistochemistry-human-spleen.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human spleen (PMID: 10464253). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-4-anti-s100a12cgrpantibody-epr23677111-immunocytochemistry-human-pbmc.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized human PBMC cells labelling S100A12/CGRP with ab272713 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMC cell line. ab195889 anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.
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![Flow Cytometry (Intracellular) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-5-anti-s100a12cgrpantibody-epr23677111-flow-cytometry-human-peripheral-blood-mononuclear-cell.jpg)
Flow Cytometry (Intracellular) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) cells labeling S100A12/CGRP with ab272713 at 1/400 dilution (0.1µg) (Right panel), compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-6-anti-s100a12cgrpantibody-epr23677111-immunoprecipitation-human-lymph-node-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)S100A12/CGRP was immunoprecipitated from 0.35 mg human lymph node tissue lysate 10 µg with ab272713 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab272713 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human lymph node tissue lysate 10 µg.
Lane 2: ab272713 IP in Human lymph node tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab272713 in human lymph node tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-2-anti-s100a12cgrpantibody-epr23677111-immunohistochemistry-human-bladder-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human bladder cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-3-anti-s100a12cgrpantibody-epr23677111-immunohistochemistry-human-cervical-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling S100A12/CGRP with ab272713 at 1/5000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulocytes of human cervical cancer (PMID: 31993369). The section was incubated with ab272713 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)](https://www.abcam.com/ps/products/272/ab272713/Images/ab272713-8-benefits-of-recombinant-antibodies.png)
Anti-S100A12/CGRP antibody [EPR23677-111] (ab272713)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab272713 has not yet been referenced specifically in any publications.