Anti-S100A4 antibody [1F12-1G7] (ab93283)
Key features and details
- Mouse monoclonal [1F12-1G7] to S100A4
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-S100A4 antibody [1F12-1G7]
See all S100A4 primary antibodies -
Description
Mouse monoclonal [1F12-1G7] to S100A4 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.
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Positive control
- HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.
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General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
1F12-1G7 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 6275 Human
- Entrez Gene: 20198 Mouse
- Omim: 114210 Human
- SwissProt: P26447 Human
- SwissProt: P07091 Mouse
- Unigene: 654444 Human
- Unigene: 3925 Mouse
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Alternative names
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
Images
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 37.22 kDa why is the actual band size different from the predicted?Predicted band size for the immunogen in 37.22 kDa.
This image was generated using the ascites version of the product.
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDaThis image was generated using the ascites version of the product.
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All lanes : Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)
Lane 1 : cell lysate from S100A4 transfected 293T cell line
Lane 2 : cell lysate from non transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 11.7 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.
This image was generated using the ascites version of the product.
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Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab93283 has been referenced in 7 publications.
- Jia M et al. Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis. Front Physiol 13:778479 (2022). PubMed: 35309056
- Gundlach JP et al. Paracrine Interaction of Cholangiocellular Carcinoma with Cancer-Associated Fibroblasts and Schwann Cells Impact Cell Migration. J Clin Med 11:N/A (2022). PubMed: 35628911
- Ugai K et al. Inhibition of the SET8 Pathway Ameliorates Lung Fibrosis Even Through Fibroblast Dedifferentiation. Front Mol Biosci 7:192 (2020). PubMed: 32850975
- Méndez-Barbero N et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500
- Nguy?n-Thanh T et al. Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice. Int J Mol Med 41:95-106 (2018). PubMed: 29115561
- Zhang W et al. S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis. Front Immunol 9:1216 (2018). PubMed: 29910813
- Wilding C et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). PubMed: 24595072