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Anti-S100A4 antibody [1F12-1G7] (ab93283)

Reviews (1)Q&A (1)References (7)
Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
  • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (ab93283)
  • ELISA - Anti-S100A4 antibody [1F12-1G7] (ab93283)

Key features and details

  • Mouse monoclonal [1F12-1G7] to S100A4
  • Suitable for: ELISA, WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-S100A4 antibody [1F12-1G7]
    See all S100A4 primary antibodies

  • Description

    Mouse monoclonal [1F12-1G7] to S100A4

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Human, Recombinant fragment

  • Immunogen

    Recombinant full length protein corresponding to amino acids 1-102 (MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT) of Human S100A4 (AAH16300) with a proprietary tag.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa cell and NIH/3T3 lysates. Transfected 293T cell line.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab93283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
ICC/IF (1)
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
ICC/IF
Use at an assay dependent concentration.

Target

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Information by UniProt

  • Database links

    • Alternative names

      • 18A2 antibody
      • 42A antibody
      • calcium Placental protein antibody
      • Calvasculin antibody
      • CAPL antibody
      • Fibroblast specific protein 1 (FSP1) antibody
      • Fibroblast specific protein 1 antibody
      • Fibroblast specific protein antibody
      • FSP1 antibody
      • Leukemia multidrug resistance associated protein antibody
      • Malignant transformation suppression 1 (MTS1) antibody
      • Malignant transformation suppression 1 antibody
      • Metastasin antibody
      • MTS1 antibody
      • OTTHUMP00000015467 antibody
      • OTTHUMP00000015468 antibody
      • P9KA antibody
      • PEL98 antibody
      • Placental calcium-binding protein antibody
      • Protein Mts1 antibody
      • Protein S100 A4 antibody
      • Protein S100-A4 antibody
      • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
      • S100 calcium binding protein A4 antibody
      • S100 calcium-binding protein A4 antibody
      • S100a4 antibody
      • S10A4_HUMAN antibody
      see all

    Images

    • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate

      Developed using the ECL technique.

      Predicted band size: 12 kDa
      Observed band size: 37.22 kDa why is the actual band size different from the predicted?



      Predicted band size for the immunogen in 37.22 kDa.

      This image was generated using the ascites version of the product.

    • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + HeLa cell lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate

      Developed using the ECL technique.

      Predicted band size: 12 kDa



      This image was generated using the ascites version of the product.

    • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer) + NIH/3T3 cell lysate at 50 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate

      Developed using the ECL technique.

      Predicted band size: 12 kDa



      This image was generated using the ascites version of the product.

    • Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Western blot - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      All lanes : Anti-S100A4 antibody [1F12-1G7] (ab93283) at 5 µg/ml (in 5% non-fat milk/PBST (0.2%) blocking buffer)

      Lane 1 : cell lysate from S100A4 transfected 293T cell line
      Lane 2 : cell lysate from non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate

      Developed using the ECL technique.

      Predicted band size: 12 kDa
      Observed band size: 11.7 kDa why is the actual band size different from the predicted?



      This image was generated using the ascites version of the product.

    • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [1F12-1G7] (ab93283)

      ab93283 at 15µg/ml staining S100A4 in fixed and permeabilised HeLa cells by immunofluorescence.

      This image was generated using the ascites version of the product.

    • ELISA - Anti-S100A4 antibody [1F12-1G7] (ab93283)
      ELISA - Anti-S100A4 antibody [1F12-1G7] (ab93283)

      Detection limit for recombinant GST tagged S100A4 is 0.1 ng/ml as a capture antibody.

    References (7)

    Publishing research using ab93283? Please let us know so that we can cite the reference in this datasheet.

    ab93283 has been referenced in 7 publications.

    • Jia M  et al. Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis. Front Physiol 13:778479 (2022). PubMed: 35309056
    • Gundlach JP  et al. Paracrine Interaction of Cholangiocellular Carcinoma with Cancer-Associated Fibroblasts and Schwann Cells Impact Cell Migration. J Clin Med 11:N/A (2022). PubMed: 35628911
    • Ugai K  et al. Inhibition of the SET8 Pathway Ameliorates Lung Fibrosis Even Through Fibroblast Dedifferentiation. Front Mol Biosci 7:192 (2020). PubMed: 32850975
    • Méndez-Barbero N  et al. A major role of TWEAK/Fn14 axis as a therapeutic target for post-angioplasty restenosis. EBioMedicine 46:274-289 (2019). PubMed: 31395500
    • Nguy?n-Thanh T  et al. Inhibition of histone deacetylase 1 ameliorates renal tubulointerstitial fibrosis via modulation of inflammation and extracellular matrix gene transcription in mice. Int J Mol Med 41:95-106 (2018). PubMed: 29115561
    • Zhang W  et al. S100a4 Is Secreted by Alternatively Activated Alveolar Macrophages and Promotes Activation of Lung Fibroblasts in Pulmonary Fibrosis. Front Immunol 9:1216 (2018). PubMed: 29910813
    • Wilding C  et al. ?-Synuclein antibodies have neuroprotective potential on neuroretinal cells via proteins of the mitochondrial apoptosis pathway. PLoS One 9:e90737 (2014). PubMed: 24595072

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-S100A4 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (differentiated cell)
    Specification
    differentiated cell
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - TritonX
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 26°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 12 2012

    Question

    Phone call requesting advice on which antibodies to choose in setting up a Sandwich ELISA for S100A4 with mouse samples.

    Read More
    Answer

    Thank you for contacting us and your interest in our products.

    I have looked into the three antibodies we have directed against S100A4 and whether they are likely to work when used in a sandwich ELISA. We have the following antibodies directed against S100A4 which have been used in ELISA previously:

    Mouse monoclonal to S100A4 (ab93283)

    Mouse monoclonal [NJ4F3] to S100A4 (ab68124)

    Goat polyclonal to S100A4 (ab115699)

    Of these, only ab93283 has been used with mouse samples previously. However, as the immunogens used to raise ab68124 and ab115699 shares high homology with the mouse protein we would expect them to cross react. As we have not tested it ourselves we would however not be able to guarantee this.

    None of the antibodies have been used as a pair in sandwich ELISA before and we would therefore not be able to guarantee that they would work together. The two mouse monoclonal antibodies are raised against immunogens which cover most of the protein length, including the region used to produce the goat polyclonal. Theoretically, the antibodies could therefore bind the same region and not be compatible. However, as we have not tested it, we would not be able to say.

    If you would like to test ab93283 and ab115699 as a pair in a sandwich ELISA you would be eligable for a testing discount. This offer means that if you purchaseab93283 and ab115699 as normal, test the antibodies with yourmouse samples in ELISA and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price ofab93283or ab115699 off any product). More information on this offer can be found here:

    www.abcam.com/collaborationdiscount

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab93283 and ab115699.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

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