Anti-S100A4 antibody (ab58597)
Key features and details
- Goat polyclonal to S100A4
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-S100A4 antibody
See all S100A4 primary antibodies -
Description
Goat polyclonal to S100A4 -
Host species
Goat -
Specificity
Both variants (NP_002952.1; NP_062427.1) represent identical protein. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog
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Immunogen
Synthetic peptide:
CNEFFEGFPDKQP
, corresponding to C terminal amino acids 86-98 of Human S100A4 -
Positive control
- Human Placenta lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab58597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 12 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 403787 Dog
- Entrez Gene: 6275 Human
- Entrez Gene: 20198 Mouse
- Entrez Gene: 24615 Rat
- Omim: 114210 Human
- SwissProt: Q9TV56 Dog
- SwissProt: P26447 Human
- SwissProt: P07091 Mouse
see all -
Alternative names
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
Images
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Western blot - Anti-S100A4 antibody (ab58597)Anti-S100A4 antibody (ab58597) at 0.03 µg/ml + Peripheral blood mononuclear cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 12 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody (ab58597)ab58597 at a concentration of 2µg/ml staining S100A4 in Human small intestine tissue sections by immunohistochemistry (paraffin-embedded). Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Protocols
Datasheets and documents
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Datasheet download
References (4)
ab58597 has been referenced in 4 publications.
- Xiong Y et al. Rutaecarpine prevents the malignant biological properties of breast cancer cells by the miR-149-3p/S100A4 axis. Ann Transl Med 10:930 (2022). PubMed: 36172090
- Jiang D et al. Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin. Nat Commun 11:5653 (2020). PubMed: 33159076
- Lerbs T et al. CD47 prevents the elimination of diseased fibroblasts in scleroderma. JCI Insight 5:N/A (2020). PubMed: 32814713
- Rasanen K et al. Comparative secretome analysis of epithelial and mesenchymal subpopulations of head and neck squamous cell carcinoma identifies S100A4 as a potential therapeutic target. Mol Cell Proteomics 12:3778-92 (2013). PubMed: 24037664
