Anti-S100A4 antibody [S100A4/1481] (ab218511)
![Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-465691-anti-s100a4-antibody-s100a41481-western-blot-wildtype-hela-s100a4-knockout-hela.jpg "Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)")
Key features and details
- Mouse monoclonal [S100A4/1481] to S100A4
- Suitable for: Protein Array, Flow Cyt, WB, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-S100A4 antibody [S100A4/1481]
See all S100A4 primary antibodies -
Description
Mouse monoclonal [S100A4/1481] to S100A4 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, Flow Cyt, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant full length protein corresponding to Human S100A4.
Database link: P26447 -
Positive control
- IHC-P: Human placenta tissue. WB: HeLa, A549 and A375 cell lysates. Flow Cyt: T98G cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S100A4/1481 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array |
Use at an assay dependent concentration.
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| Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa.
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| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
Protein Array
Use at an assay dependent concentration. |
|
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 12 kDa. |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. - Information by UniProt
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Database links
- Entrez Gene: 6275 Human
- Entrez Gene: 20198 Mouse
- Omim: 114210 Human
- SwissProt: P26447 Human
- SwissProt: P07091 Mouse
- Unigene: 654444 Human
- Unigene: 3925 Mouse
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Alternative names
- 18A2 antibody
- 42A antibody
- calcium Placental protein antibody
see all
Images
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Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 0.5 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : S100A4 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab218511 observed at 11 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab218511 was shown to react with S100A4 in wild-type HeLa cells in Western blot with loss of signal observed in S100A4 knockout cell line ab265709 (S100A4 knockout cell lysate ab257046). Wild-type HeLa and S100A4 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab218511 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-386630-anti-s100a4-antibody-s100a41481-western-blot-human-lysate.jpg)
Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : S100A4 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaLanes 1 - 4: Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control ab181602 observed at 37 kDa.
ab218511 Anti-S100A4 antibody [S100A4/1481] was shown to specifically react with S100A4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261758 (knockout cell lysate ab257045) was used. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab218511 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-274178-anti-s100a4-antibody-s100a4-1481-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [S100A4/1481] (ab218511)Immunohistochemical analysis of formalin-fixed paraffin-embedded human placenta tissue, labeling S100A4 using ab218511 at 1 µg/mL.
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![Flow Cytometry - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-456429-anti-s100a4-antibody-flow-cytometry-t98g-human.jpg)
Flow Cytometry - Anti-S100A4 antibody [S100A4/1481] (ab218511)Flow Cytometric Analysis of human T98G cells using ab218511 at 1 ug/ml, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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![Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-345770-anti-s100a4-antibody-s100a41481-westernblot-human.jpg)
Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/ml
Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : S100A4 knockout A549 (Human lung carcinoma cell line) whole cell lysate
Lane 3 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 12 kDa
Observed band size: 12 kDaLanes 1 - 4: Merged signal (red and green). Green - ab218511 observed at 12 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab218511 was shown to specifically react with S100A4 in wild-type A549 cells as signal was lost in S100A4 knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. Ab218511 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Protein Array - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-336517-anti-s100a4-antibody-s100a4-1481-protein-array.jpg)
Protein Array - Anti-S100A4 antibody [S100A4/1481] (ab218511)ab218511 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)](https://www.abcam.com/ps/products/218/ab218511/Images/ab218511-274179-anti-s100a4-antibody-s100a4-1481-western-blot.jpg)
Western blot - Anti-S100A4 antibody [S100A4/1481] (ab218511)All lanes : Anti-S100A4 antibody [S100A4/1481] (ab218511) at 1 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A375 cell lysate
Predicted band size: 12 kDa
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab218511 has been referenced in 1 publication.
- Shiozawa J et al. Implication of HYBID (Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization) in Hyaluronan Degradation by Synovial Fibroblasts in Patients with Knee Osteoarthritis. Am J Pathol 190:1046-1058 (2020). PubMed: 32084364
