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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SA2 antibody [EPR10994(B)] (ab155081)

Submit a review Submit a question References (1)
Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
  • Anti-SA2 antibody [EPR10994(B)] (ab155081)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10994(B)] to SA2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SA2 antibody [EPR10994(B)]
    See all SA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR10994(B)] to SA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide within Human SA2 aa 1200 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N3U4

  • Positive control

    • WB: HCT116, HeLa, K562, MCF7 and Jurkat whole cell lysate (ab7899). IHC-P: Human colon tissue and gastric adenocarcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab155081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 141 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 141 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

  • Sequence similarities

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.

  • Post-translational
    modifications

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.

  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all

Images

  • Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    All lanes : Anti-SA2 antibody [EPR10994(B)] (ab155081) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : STAG2 knockout HeLa cell lysate
    Lane 3 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 141 kDa
    Observed band size: 141 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab155081 observed at 141 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab155081 Anti-SA2 antibody [EPR10994(B)]  was shown to specifically react with SA2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265461 (knockout cell lysate ab257707) was used.  Wild-type and SA2 knockout samples were subjected to SDS-PAGE.  ab155081 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SA2 with ab155081 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Western blot - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    All lanes : Anti-SA2 antibody [EPR10994(B)] (ab155081) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 141 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

    Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling SA2 with ab155081 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

    Immunohistochemical analysis of paraffin embedded Human normal spleen tissue using ab155081 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

    Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab155081 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

    Immunohistochemical analysis of paraffin embedded Human melanoma tissue using ab155081 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SA2 antibody [EPR10994(B)] (ab155081)

    Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155081 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-SA2 antibody [EPR10994(B)] (ab155081)
    Anti-SA2 antibody [EPR10994(B)] (ab155081)

References (1)

Publishing research using ab155081? Please let us know so that we can cite the reference in this datasheet.

ab155081 has been referenced in 1 publication.

  • Reichmann J  et al. Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. J Cell Biol 219:N/A (2020). PubMed: 32232464

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