Recombinant Anti-Sall4 antibody [EPR10642] - BSA and Azide free (ab250336)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [EPR10642] - BSA and Azide free (ab250336)](https://www.abcam.com/ps/products/250/ab250336/Images/ab250336-1-anti-sall4-antibody-epr10642-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [EPR10642] - BSA and Azide free (ab250336)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10642] to Sall4 - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Sall4 antibody [EPR10642] - BSA and Azide free
See all Sall4 primary antibodies -
Description
Rabbit monoclonal [EPR10642] to Sall4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250336 is the carrier-free version of ab181087.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10642 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Probable transcription factor. -
Tissue specificity
Expressed in testis. -
Involvement in disease
Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype. -
Sequence similarities
Belongs to the sal C2H2-type zinc-finger protein family.
Contains 7 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57167 Human
- Omim: 607343 Human
- SwissProt: Q9UJQ4 Human
- Unigene: 517113 Human
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Alternative names
- AA407717 antibody
- AL022809 antibody
- AW536104 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [EPR10642] - BSA and Azide free (ab250336)This data was developed using ab181087, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human yolk sac carcinoma tissue labeling Sall4 with ab181087 at a 1/100 dilution. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Anti-Sall4 antibody [EPR10642] - BSA and Azide free (ab250336)](https://www.abcam.com/ps/products/250/ab250336/Images/ab250336-2-benefits-of-recombinant-antibodies.png)
Anti-Sall4 antibody [EPR10642] - BSA and Azide free (ab250336)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250336 has not yet been referenced specifically in any publications.
