Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-Sall4 antibody [SP289] (ab226756)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] (ab226756)
  • Flow Cytometry (Intracellular) - Anti-Sall4 antibody [SP289] (ab226756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
  • Anti-Sall4 antibody [SP289] (ab226756)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP289] to Sall4
  • Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-Sall4 antibody [SP289]
    See all Sall4 primary antibodies

  • Description

    Rabbit monoclonal [SP289] to Sall4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human seminoma, testis, and Human ovarian carcinoma tissue; Flow Cyt (intra): NCCIT cells; ICC: NCCIT cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab226756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
Flow Cyt (Intra)
1/20.
ICC/IF
1/10.
Notes
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
Flow Cyt (Intra)
1/20.
ICC/IF
1/10.

Target

  • Function

    Probable transcription factor.

  • Tissue specificity

    Expressed in testis.

  • Involvement in disease

    Defects in SALL4 are the cause of Duane-radial ray syndrome (DRRS) [MIM:607323]; also known as Okihiro syndrome. DRRS is a disorder characterized by the association of forearm malformations with Duane retraction syndrome.
    Defects in SALL4 are the cause of oculootoradial syndrome (OORS) [MIM:147750]. Oculootoradial syndrome is an autosomal dominant condition characterized by upper limbs anomalies (radial ray defects, carpal bones fusion), extraocular motor disturbances, congenital bilateral non-progressive mixed hearing loss. Other less consistent malformations include heart involvement, mild thrombocytopenia and leukocytosis (before age 50), shoulder girdle hypoplasia, imperforate anus, kidney malrotation or rectovaginal fistula. The IVIC syndrome is an allelic disorder of Duane-radial ray syndrome (DRRS) with a similar phenotype.

  • Sequence similarities

    Belongs to the sal C2H2-type zinc-finger protein family.
    Contains 7 C2H2-type zinc fingers.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • AA407717 antibody
      • AL022809 antibody
      • AW536104 antibody
      • C330011P20Rik antibody
      • C78083 antibody
      • C78563 antibody
      • dJ1112F19.1 antibody
      • DRRS antibody
      • HSAL4 antibody
      • Sal like 4 (Drosophila) antibody
      • Sal like 4 antibody
      • Sal like Protein 4 antibody
      • Sal-like protein 4 antibody
      • Sall4 antibody
      • SALL4_HUMAN antibody
      • Spalt like transcription factor 4 antibody
      • Tex20 antibody
      • Zinc finger protein 797 antibody
      • Zinc finger protein SALL4 antibody
      • ZNF797 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human ovarian carcinoma tissue sections labeling Sall4 with ab226756 at 1/100 dilution (1.23 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] (ab226756)
      Immunocytochemistry/ Immunofluorescence - Anti-Sall4 antibody [SP289] (ab226756)

      Immunocytochemistry/ Immunofluorescence analysis of NCCIT (human pluripotent embryonic carcinoma epithelial cell) cells labeling Sall4 with purified ab226756 at 1/10 (12.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Flow Cytometry (Intracellular) - Anti-Sall4 antibody [SP289] (ab226756)
      Flow Cytometry (Intracellular) - Anti-Sall4 antibody [SP289] (ab226756)

      Intracellular Flow Cytometry analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) labeling Sall4 with purified ab226756 at 1/20 dilution (6.15µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) / Black. Unlableled control -Unlabelled cells / blue. 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

      Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

      Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

      Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

      Formalin-fixed, paraffin-embedded human seminoma tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sall4 antibody [SP289] (ab226756)

      Formalin-fixed, paraffin-embedded human testis tissue stained for Sall4 using ab226756 at 1/100 dilution in immunohistochemical analysis.

    • Anti-Sall4 antibody [SP289] (ab226756)
      Anti-Sall4 antibody [SP289] (ab226756)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab226756? Please let us know so that we can cite the reference in this datasheet.

    ab226756 has been referenced in 1 publication.

    • Sivakumar S  et al. TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling. Cell Rep 38:110395 (2022). PubMed: 35172133

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab226756.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com