Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)
Key features and details
- Rabbit polyclonal to SARS-CoV-2 spike glycoprotein - Coronavirus
- Suitable for: IHC-P, ICC/IF, ELISA, WB
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus
See all SARS-CoV-2 spike glycoprotein primary antibodies -
Description
Rabbit polyclonal to SARS-CoV-2 spike glycoprotein - Coronavirus -
Host species
Rabbit -
Specificity
The immunogen for ab272504 is within the last 50 aa of the spike protein - a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The Extracellular domain (ECD) is from aa 1 to 1208 (full length 1273aa). Therefore, this antibody detects the transmembrane and cytoplasm domains at the C terminus, but does not detect the ECD (which is the region expressed in many commercially available spike proteins). ab272504 can be used for the detection of full length spike protein and spike protein in COVID-19 patient samples. It will detect 4 ng of free peptide at 1 μg/mL.
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Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Synthetic peptide within Human coronavirus SARS-CoV-2 spike glycoprotein (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. 20 amino acid peptide within the last 50 amino acids of the SARS CoV-2 spike glycoprotein
Database link: QHD43416
(Peptide available asab273063) -
Positive control
- WB: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein, cell lysate. ICC: HEK-293 cells overexpressing SARS-CoV-2 spike glycoprotein. IHC-P: COVID-19 patient lung tissue.
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General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| ELISA |
Use a concentration of 1 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Can be blocked with Human coronavirus SARS-CoV-2 spike glycoprotein peptide (ab273063).
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| Notes |
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IHC-P
Use a concentration of 0.2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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ELISA
Use a concentration of 1 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Can be blocked with Human coronavirus SARS-CoV-2 spike glycoprotein peptide (ab273063). |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)
Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages and airway epithelium of COVID-19 patient lung but not in non-COVID-19 patient lung.
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Immunocytochemistry/ Immunofluorescence - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunofluorescent analysis of 4% paraformaldehyde-fixed 293 spike transfected cells labelling SARS-CoV-2 (Covid-19) Spike with ab272504 at 1 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
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Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504) at 1 µg/ml
Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : HEK-293 overexpressing SARS-CoV-2 spike glycoprotein, cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilutionPrimary incubation for 1 hour at room temperature.
Blocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Spike S2 antibody (ab272504, 0.5 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong spike protein signal was observed in macrophages of COVID-19 patient lung.
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ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody - Coronavirus (ab272504)A direct ELISA was performed using antigen or control peptide as coating antigen and ab272504 as the capture antibody at 1 μg/ml, followed by the secondary antibody goat anti-rabbit IgG HRP conjugate at 1/20000 dilution. Detection range was from 0.5 ng/ml to 1000 ng/ml.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab272504 has been referenced in 34 publications.
- Boix-Besora A et al. Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2. Vaccines (Basel) 10:N/A (2022). PubMed: 35214708
- Jakovac H et al. Detection of Sars-Cov-2 antigens in thyroid gland showing histopathological features of subacute thyroiditis. Eur Thyroid J 11:N/A (2022). PubMed: 35166214
- Botosso VF et al. Anti-SARS-CoV-2 equine F (Ab')2 immunoglobulin as a possible therapy for COVID-19. Sci Rep 12:3890 (2022). PubMed: 35273234
- Jakovac H et al. Detection of SARS-CoV-2 Antigens in the AV-Node of a Cardiac Conduction System-A Case Report. Trop Med Infect Dis 7:N/A (2022). PubMed: 35324590
- Lai X et al. Identified human breast milk compositions effectively inhibit SARS-CoV-2 and variants infection and replication. iScience 25:104136 (2022). PubMed: 35342878