Recombinant Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
![Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)](https://www.abcam.com/ps/products/272/ab272854/Images/ab272854-415068-anti-sars-cov-2-spike-glycoprotein-antibody-h6-chimeric-western-blot-mock-transfected-expi-cells-pdl1-flag-transfected-expi-cells-sarscov1.jpg "Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric
- Suitable for: ELISA, WB, Functional Studies
Related conjugates and formulations
Overview
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Product name
Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric)
See all SARS-CoV-2 spike glycoprotein primary antibodies -
Description
Human monoclonal [H6] to SARS-CoV-2 spike glycoprotein - Chimeric -
Host species
Human -
Specificity
We have in-house data that suggests this product also reacts with SARS-CoV-1 spike protein in Western blot.
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Tested applications
Suitable for: ELISA, WB, Functional Studiesmore details -
Species reactivity
Reacts with: SARS-CoV, SARS-CoV-2 -
Immunogen
Recombinant fragment corresponding to Human SARS-CoV-2 spike glycoprotein aa 16-685.
Database link: P0DTC2 -
Positive control
- WB: SARS-Cov1 spike protein transfected Expi cell lysate, SARS-Cov1 3xFlag spike protein transfected Expi cell lysate, SARS-Cov2 spike protein transfected Expi cell lysate and SARS-Cov2 3xFlag spike protein transfected Expi cell lysate.
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General notes
Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) is a recombinant human/mouse chimeric monoclonal antibody consisting of Mouse scFv fused with human IgG1.
As the Fc region is human, an anti-Human secondary antibody should be used for detection. A suitable anti-Mouse secondary may also be used.
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
>90% by SDS-PAGE -
Purification notes
Screened the positive clones from mouse ScFv antibody libraries, then expressed in 293F cells. -
Clonality
Monoclonal -
Clone number
H6 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | (1) |
1/10000 - 1/50000.
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| WB |
Use a concentration of 1 µg/ml.
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| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
|---|
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ELISA
1/10000 - 1/50000. |
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WB
Use a concentration of 1 µg/ml. |
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Functional Studies
Use at an assay dependent concentration. |
Images
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Western blot - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)All lanes : Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) at 1 µg/ml
Lane 1 : Mock transfected Expi cell lysate
Lane 2 : PDL-1 Flag transfected Expi cell lysate
Lane 3 : SARS-Cov1 spike protein transfected Expi cell lysate
Lane 4 : SARS-Cov1 3xFlag spike protein transfected Expi cell lysate
Lane 5 : SARS-Cov2 spike protein transfected Expi cell lysate
Lane 6 : SARS-Cov2 3xFlag spike protein transfected Expi cell lysate
Lane 7 : MERS Spike protein transfected Expi cell lysate
Lane 8 : MERS 3xFlag Spike protein transfected Expi cell lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Observed band size: 200 kDa why is the actual band size different from the predicted?Lanes 1 - 8: Merged signal (red and green). Green - ab272854 observed at 200 kDa. Red - loading control.
ab272854 was shown to react with SARS-CoV-2 spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab272854 overnight at 4°C at 1 ug/ml. Blots were incubated with Donkey anti-Mouse IgG H&L(IRDye® 800CW) preabsorbed (ab216774) antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Note: ab216774 is suitable for the recognition of the Mouse scFv fragment of ab272854.
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![ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)](https://www.abcam.com/ps/products/272/ab272854/Images/ab272854-390098-anti-sars-cov-2-spike-glycoprotein-antibody-h6-coronavirus-ELISA.jpg)
ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)Binding Activity of ab272854 with SARS-CoV-2 spike glycoprotein.
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2 spike glycoprotein at 2 μg/ml can bind ab272854. The EC50 of ab272854 is 36.79-48.87 ng/ml. -
![ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)](https://www.abcam.com/ps/products/272/ab272854/Images/ab272854-390947-anti-sars-cov2-spike-glycoprotein-antibody-h6-elisa.jpg)
ELISA - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)Binding Activity of ab272854 with SARS-CoV-2-S1-RBD
Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 μg/ml can bind ab272854, the EC50 of SARS-CoV-2-S Antibody is 19.60-39.42 ng/ml. -
![Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)](https://www.abcam.com/ps/products/272/ab272854/Images/ab272854-394100-anti-sars-cov-2-spike-glycoprotein-antibody-h6-chimeric-functional-studies.jpg)
Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 23.32 nM.
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![Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)](https://www.abcam.com/ps/products/272/ab272854/Images/ab272854-394101-anti-sars-cov-2-spike-glycoprotein-antibody-h6-chimeric-functional-studies-inhibition.jpg)
Functional Studies - Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)Binding signal of Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854) and SARS-CoV-2-S1-RBD was inhibited by ACE2 protein-HRP conjugated inhibitor with the IC50 2.38 µg/mL.
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![Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)](https://www.abcam.com/ps/products/272/ab272854/Images/ab272854-8-benefits-of-recombinant-antibodies.png)
Anti-SARS-CoV-2 spike glycoprotein antibody [H6] - Human IgG1 (Chimeric) (ab272854)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab272854 has been referenced in 1 publication.
- Boix-Besora A et al. Optimization, Production, Purification and Characterization of HIV-1 GAG-Based Virus-like Particles Functionalized with SARS-CoV-2. Vaccines (Basel) 10:N/A (2022). PubMed: 35214708