Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)
![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)](https://www.abcam.com/ps/products/282/ab282749/Images/ab282749-512699-anti-sars-cov-2-spike-glycoprotein-s1-antibody-8b12-c2-bsa-and-azide-free-western-blot-recombinant-human-coronavirus-sarscov2-spike-glycoprotein-s1-active-h.jpg "Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [8B12-C2] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
- Suitable for: WB, ELISA
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Mouse monoclonal [8B12-C2] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8B12-C2 -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab282749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500.
|
|
| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/500. |
|
ELISA
Use at an assay dependent concentration. |
Images
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Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)Lanes 1-4 : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749) at 1/500 dilution
Lanes 5-8 : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/1000 dilution
Lanes 1 & 5 : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active, His tagged) (ab273068) cell lysate
Lanes 2 & 6 : Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein RBD (His tag) (ab275986) cell lysate
Lanes 3 & 7 : SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein cell lysate
Lanes 4 & 8 : SARS-CoV Spike S1+S2 ECD-His Recombinant Protein cell lysate
Lysates/proteins at 0.2 µg per lane.
Performed under reducing conditions.
Observed band size: 28-90-180 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free staining at 1/500 dilution, shown in green; Mouse anti-6X His Tag® [HIS.H8] (ab18184) loading control staining at 1/1000 dilution, shown in red. In Western blot, ab282749 was shown to bind specifically to SARS-CoV-2 Spike Glycoprotein S1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) and Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) at 1/20000 dilution.
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![Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)](https://www.abcam.com/ps/products/282/ab282749/Images/ab282749-477711-ab282749.png)
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)Lanes 1-3 : anti-P2A at 1/500 dilution
Lanes 4-6 : Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749) at 0.1 µg/ml
Lanes 1 & 4 : HEK293T with 3% NFDM in PBS-T
Lanes 2 & 5 : + Spike WT with 3% NFDM in PBS-T
Lanes 3 & 6 : + Spike B1.351 with 3% NFDM in PBS-T
Blocking peptides at 0.05 % per lane.
Secondary
All lanes : HRP-coupledWestern blot - Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] (ab282749) Whole cell lysates of HEK293T cells expressing Sars-CoV2 Spike-P2A-GFP protein either as the wild-type (Wt) or the B 1.351 variant version were separated by 7.5% SDS-PAGE and transferred to nitrocellulose membrane. Membranes were blocked in 3% NFDM in PBS-T (0.05% Tween) for 1h at RT and incubated with anti-P2A (3H4-3A5, 1:500) or ab282749 (0,1µg/ml) diluted in 0.5% NFDM/PBS-T o/n at 4°C. After washing and incubation with HRP-coupled secondary antibody for 1h at RT, signals were visualized by ECL reaction and Chemidoc or X-ray film detection.
This data was produced by a Collaborator. -
![ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)](https://www.abcam.com/ps/products/282/ab282749/Images/ab282749-472387-AntiSARSCoV2-Spike-Glycoprotein-S1-antibody-8B12C2-elisa.jpg)
ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105) and Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) at 1000 ng/ml.
Primary antibody (ab282749) concentration range 0.045-100 ng/ml.
Secondary antibody, Peroxidase AffiniPure Goat Anti-Mouse IgG, Fcγ Fragment Specific used at 1/2000 dilution. -
![Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)](https://www.abcam.com/ps/products/282/ab282749/Images/ab282749-2-Benefits-of-Recombinant-antibodies.jpg)
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - BSA and Azide free (ab282749)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab282749 has not yet been referenced specifically in any publications.
