Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric) (ab273886)
![Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric) (ab273886)](https://www.abcam.com/ps/products/273/ab273886/Images/ab273886-456415-AntiSARSCoV2-Spike-Glycoprotein-S1-antibody-CR3022-Chimeric-indirect-elisa-recombinant.jpg "Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric) (ab273886)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [CR3022] to SARS-CoV-2 Spike Glycoprotein S1 - Chimeric
- Suitable for: Indirect ELISA
Related conjugates and formulations
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric)
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Rat monoclonal [CR3022] to SARS-CoV-2 Spike Glycoprotein S1 - Chimeric -
Host species
Rat -
Tested applications
Suitable for: Indirect ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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General notes
This rat monoclonal chimeric antibody (ab273886) was developed to have a rat IgG2a isotype.
Other isotypes of clone CR3022 available:
ab278112 – human IgA
ab273073 – human IgG1
ab273074 – rabbit IgG
ab278111 – human IgMThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. -
Storage buffer
Preservative: 0.02% Proclin 300 Solution
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
CR3022 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Indirect ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Indirect ELISA
Use at an assay dependent concentration. |
Images
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Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric) (ab273886)
Indirect ELISA showing rat chimeric ab273886 (CR3022) binding to ab272105 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (sheep Fc fusion)).
Plates were coated with 100ng/well ab272105 and binding of ab273886 assessed in serial dilution from 200ng/ml primary antibody in quadruplicate. Binding was detected using a pre-adsorbed anti-rat IgG Fc secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
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![Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric) (ab273886)](https://www.abcam.com/ps/products/273/ab273886/Images/ab273886-456416-AntiSARSCoV2-Spike-Glycoprotein-S1-antibody-CR3022-Chimeric-indirect-elisa-recombinant2.jpg)
Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] – Rat IgG2a (Chimeric) (ab273886)Indirect ELISA showing rat chimeric ab273886 (CR3022) binding to ab273068 (recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active)).
Plates were coated with 100ng/well ab272105 and binding of ab273886 assessed in serial dilution from 200ng/ml primary antibody in quadruplicate. Binding was detected using a pre-adsorbed anti-rat IgG Fc secondary conjugated to HRP. Data are represented as the mean and error bars represent standard deviation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab273886 has not yet been referenced specifically in any publications.