Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] (ab278111)
![ELISA - Anti-SARS spike glycoprotein + COVID-19 Spike Protein antibody [CR3022] (ab278111)](https://www.abcam.com/ps/products/278/ab278111/Images/ab278111-459618-anti-sars-spike-glycoprotein-covid-19-spike-protein-antibody-cr3022-elisa.jpg "ELISA - Anti-SARS spike glycoprotein + COVID-19 Spike Protein antibody [CR3022] (ab278111)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [CR3022] to SARS-CoV-2 Spike Glycoprotein S1
- Suitable for: ELISA
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022]
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Human monoclonal [CR3022] to SARS-CoV-2 Spike Glycoprotein S1 -
Host species
Human -
Specificity
This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. 2020).
Reacts with: SARS-CoV, SARS-CoV-2
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: SARS-CoV, SARS-CoV-2 -
Immunogen
Tissue, cells or virus corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV.
Database link: P59594 -
General notes
This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
This antibody does not have a J-chain and therefore presents as a hexamer, rather than a pentamer.
ab278111 was developed to have a human IgM isotype.
Other isotypes of clone CR3022 available:
ab278112 – human IgA
ab273073 – human IgG1
ab273074 – rabbit IgG
ab273886 – rat IgG2aThis product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity Purified using a recombinant lectin column. -
Clonality
Monoclonal -
Clone number
CR3022 -
Isotype
IgM -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab278111 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use a concentration of 0.005 - 10 µg/ml.
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| Notes |
|---|
|
ELISA
Use a concentration of 0.005 - 10 µg/ml. |
Images
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ELISA - Anti-SARS spike glycoprotein + COVID-19 Spike Protein antibody [CR3022] (ab278111)
ELISA analysis of ab278111 binding SARS-CoV-2 Spike Glycoprotein domains S1 and S2 (His-Tag (Insect Cells)). ELISA plate coated with SARS-CoV-2 Spike Glycoprotein (S1), His-Tag (Insect Cells; grey line) and SARS-CoV-2 Spike Glycoprotein (S2), His-Tag (Insect Cells; yellow line) (Native Antigen) at concentrations of 5 µg/mL. A 3-fold serial dilution from 10000 ng/mL was performed using ab278111 conjugated to HRP.
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![Anti-SARS spike glycoprotein + COVID-19 Spike Protein antibody [CR3022] (ab278111)](https://www.abcam.com/ps/products/278/ab278111/Images/ab278111-459604-benefits-of-recombinant-antibodies.jpg)
Anti-SARS spike glycoprotein + COVID-19 Spike Protein antibody [CR3022] (ab278111)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab278111 has been referenced in 1 publication.
- ter Meulen J et al. Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants. PLoS Med 3:e237 (2006). PubMed: 16796401