Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022] (ab278112)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [CR3022] to SARS-CoV-2 Spike Glycoprotein S1
- Suitable for: ELISA
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [CR3022]
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Human monoclonal [CR3022] to SARS-CoV-2 Spike Glycoprotein S1 -
Host species
Human -
Specificity
This antibody binds the amino acids 318-510 in the S1 domain of the SARS-CoV Spike protein as well as SARS-CoV-2 (COVID-19) Spike protein. The antibody also binds to P462L-substituted S318–510 fragments of the SARS spike protein. The binding epitope is only accessible in the "open" conformation of the spike protein (Joyce et al. 2020).
Reacts with: SARS-CoV, SARS-CoV-2
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: SARS-CoV, SARS-CoV-2 -
Immunogen
Tissue, cells or virus corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1. The original monoclonal antibody was generated by sequencing peripheral blood lymphocytes of a patient exposed to the SARS-CoV.
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General notes
This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
This antibody does not have a J-chain.
ab278112 was developed to have a human IgA isotype.
Other isotypes of clone CR3022 available:
ab278111 – human IgM
ab273073 – human IgG1
ab273074 – rabbit IgG
ab273886 – rat IgG2a
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity Purified using a recombinant lectin column. -
Clonality
Monoclonal -
Clone number
CR3022 -
Isotype
IgA -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab278112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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ELISA
Use at an assay dependent concentration. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab278112 has been referenced in 1 publication.
- ter Meulen J et al. Human monoclonal antibody combination against SARS coronavirus: synergy and coverage of escape mutants. PLoS Med 3:e237 (2006). PubMed: 16796401