Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free (ab288143)
![ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free (ab288143)](https://www.abcam.com/ps/products/288/ab288143/Images/ab288143-477924-anti-sars-cov2-spike-RBD-antibody-T4P5H12-elisa.jpg "ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free (ab288143)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Llama monoclonal [T4P5-H12] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free
- Suitable for: ELISA
Overview
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Product name
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free
See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies -
Description
Llama monoclonal [T4P5-H12] to SARS-CoV-2 Spike Glycoprotein S1 - BSA and Azide free -
Host species
Llama -
Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment (His-tag) corresponding to Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 aa 300-550 (C terminal).
Database link: P0DTC2 -
General notes
Antibody is supplied as a His-tagged purified protein. It also contains a myc-tag for detection.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 0.138% Monobasic dihydrogen sodium phosphate, 0.142% Dibasic monohydrogen sodium phosphate, 1.636% Sodium chloride, 98.084% Water -
Carrier free
Yes -
Concentration information loading... -
Purity
Purified via His tag -
Clonality
Monoclonal -
Clone number
T4P5-H12 -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab288143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ELISA
Use at an assay dependent concentration. |
Images
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ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free (ab288143)
Direct ELISA using SARS-CoV2 Spike RBD recombinant protein (aa 319-541), SARS-CoV2 Spike S1 RBD recombinant protein (aa 16-685) and SARS-CoV2 Spike ECD (aa 1-1208) as coating antigen at 1 μg/mL. ab288143 was used as detection antibody, followed by an anti-c-Myc antibody at at 1 μg/mL.
Secondary antibody is a Goat anti-Mouse IgG-HRP conjugate at 1/5000 dilution.
ab288143 can detect these proteins at 2 ng/mL.
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![Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free (ab288143)](https://www.abcam.com/ps/products/288/ab288143/Images/ab288143-477923-benefits-of-recombinant-antibodies--Copy.jpg)
Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [T4P5-H12] - BSA and Azide free (ab288143)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab288143 has not yet been referenced specifically in any publications.
