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RecombinantRabMAb

Recombinant Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)

Reviews (1) Submit a question
Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24852-174] to SARS-CoV-2 Spike Glycoprotein S1
  • Suitable for: Flow Cyt (Intra), WB, Indirect ELISA

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174]
    See all SARS-CoV-2 Spike Glycoprotein S1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24852-174] to SARS-CoV-2 Spike Glycoprotein S1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, Indirect ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV-2

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human coronavirus SARS-CoV-2 Spike glycoprotein S1, Expi293 cells transfected with SARS-CoV2 ORF7. Indirect ELISA: recombinant human coronavirus SARS-CoV-2 Spike glycoprotein S1.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24852-174

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab283946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/500.
Indirect ELISA
Use a concentration of 2e-005 - 1 µg/ml.
Notes
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
1/500.
Indirect ELISA
Use a concentration of 2e-005 - 1 µg/ml.

Images

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946) at 1/500 dilution

    All lanes : Recombinant human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Active) (ab273068)

    Lysates/proteins at 0.5 µg per lane.


    Lane 1: Red – loading control Mouse anti-6x His tag antibody (ab18184) observed at 125 kDa

    Lanes 2: Green – ab283946 observed at 125 kDa

    ab283946 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/20000 dilution.

    Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T)

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)

    Indirect ELISA showing primary antibody ab283946 binding to recombinant human coronavirus SARS-CoV-2 (Omicron variant) Spike Glycoprotein S1; SARS-CoV-2 (Omicron variant) Spike Glycoprotein RBD; SARS-CoV-2 Omicron variant Spike protein (GCN4-IZ) His tag; Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera).

    Plates were coated with recombinant human coronavirus SARS-CoV-2 (Omicron variant) Spike Glycoprotein S1; SARS-CoV-2 (Omicron variant) Spike Glycoprotein RBD; SARS-CoV-2 Omicron variant Spike protein (GCN4-IZ) His tag; Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) at 1000 ng/ml. Binding of ab283946 was assessed in a serial dilution range 0 - 1000 ng/mL.

    Binding was detected using Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.

    Substrate Solution: p-nitrophenyl phosphate(PNPP)

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)

    Indirect ELISA showing primary antibody ab283946 binding to recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105), SARS-CoV-2 spike S1+S2 ECD-His recombinant protein and recombinant human coronavirus SARS-CoV-2 spike glycoprotein RBD (His tagged, ab275986) .

    Plates were coated with recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105), SARS-CoV-2 spike S1+S2 ECD-His recombinant protein, SARS-CoV spike S1+S2 ECD-His recombinant protein and recombinant human coronavirus SARS-CoV-2 spike glycoprotein RBD (His tagged, ab275986) at 1000 ng/ml. Binding of ab283946 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

    Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

  • Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    Western blot - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    All lanes : Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946) at 1/500 dilution

    Lanes 1 & 3 : Expi293 cells transfected with SARS-CoV2 3xFlag Spike Protein
    Lanes 2 & 4 : Expi293 cells transfected with SARS-CoV1 3xFlag Spike Protein

    Lysates/proteins at 40 µg per lane.


    Lane 1 & 2: Red – loading control Goat anti-DDDDK tag antibody (ab95045, Binds to FLAG tag sequence) observed at 200 kDa

    Lanes 3 & 4: Green – ab283946 observed at 200 kDa

    ab283946 was shown to bind specifically to SARS-CoV-2 spike glycoprotein S1 in Western blot. Samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preadsorbed (ab216775) and Donkey anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216779) at 1/20000 dilution.

    Blocking buffer: 3% milk in TBS-0.1% Tween® 20 (TBS-T)

  • Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)
    Indirect ELISA - Anti-SARS-CoV-2 Spike Glycoprotein S1 (RBD) antibody [EPR24852-174] (ab283946)

    Indirect ELISA showing primary antibody ab283946 binding to the antigen ab272105 (recombinant human coronavirus SARS-CoV-2 Spike glycoprotein S1 (Fc Chimera)). Plates were coated with recombinant human coronavirus SARS-CoV-2 spike glycoprotein S1 (Fc Chimera, ab272105) at 1000 ng/ml. Binding of ab283946 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution).

    Binding was detected using pre-adsorbed secondary antibody, goat anti-rabbit IgG H&L (HRP, ab97080) at 1/2000 dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab283946? Please let us know so that we can cite the reference in this datasheet.

ab283946 has not yet been referenced specifically in any publications.

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Immunocytochemistry/ Immunofluorescence abreview for Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-174]

 Inconclusive
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Syrian hamster Cell (lung tissue)
Permeabilization
Yes - 1% Triton X-100 in PBS
Specification
lung tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Formalin
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Apr 04 2022

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