Recombinant Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
![Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-2-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-indirectelisa.jpg "Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HL1014] to SARS-CoV-2 Spike RBD - BSA and Azide free
- Suitable for: ICC, Sandwich ELISA, Indirect ELISA
Overview
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Product name
Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free
See all SARS-CoV-2 Spike RBD primary antibodies -
Description
Rabbit monoclonal [HL1014] to SARS-CoV-2 Spike RBD - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Sandwich ELISA, Indirect ELISAmore details -
Species reactivity
Reacts with: SARS-CoV-2 -
Immunogen
Recombinant fragment within Human coronavirus SARS-CoV-2 Spike RBD. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Strain Wuhan-Hu-1
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Positive control
- ICC: Mock and transfected HEK-293T cells. ELISA: Recombinant full-length SARS-CoV-2 spike (trimer) protein, recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, SARS-CoV-2 (COVID-19) Spike transfected HEK-293T cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HL1014 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/100 - 1/2500.
|
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| Sandwich ELISA |
Use at an assay dependent concentration.
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| Indirect ELISA |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
ICC
1/100 - 1/2500. |
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Sandwich ELISA
Use at an assay dependent concentration. |
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Indirect ELISA
Use at an assay dependent concentration. |
Images
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Indirect ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)
Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 spike (trimer) protein (50 ng). Coated protein probed with ab281305 (160-1.91*10-5 nM). HRP-Rabbit IgG antibody at 1/10000 dilution detected bound primary antibody. EC50 : 28.77 pM.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-456893-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-elisa-2.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and a HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.
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![Immunocytochemistry - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-3-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-immunocytochemistry-293tcells.jpg)
Immunocytochemistry - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Mock and transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate were fixed in 4% paraformaldehyde at room temperature for 15 minutes. SARS-CoV-2 (COVID-19) Spike RBD stained (green) by ab281305 at 1/2500 dilution immunocytochemical analysis. Fluoroshield with DAPI (blue).
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-456901-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-elisa.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of recombinant full-length SARS-CoV-2 spike (trimer) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-456908-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-elisa-5.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike (HexaPro) ECD, His tag using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody as detection antibody at concentration of 1 μg/mL.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-456909-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-elisa-6.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody as detection antibody at concentration of 1 μg/mL.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-1-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-sandwichelisa.jpg)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike RBD (N501Y Mutant) protein, His tag (active) using ab281305 as capture antibody at 5 µg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 µg/mL.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-456902-SARSCoV2-COVID19-Spike-RBD-antibody-HL1014-elisa-3.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) Spike RBD antibody as detection antibody at concentration of 1 μg/mL.
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![Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)](https://www.abcam.com/ps/products/281/ab281305/Images/ab281305-456903-SARSCoV2-COVID19-Spike-RBD-antibody-HL1004-elisa-4.JPG)
Sandwich ELISA - Anti-SARS-CoV-2 Spike RBD antibody [HL1014] - BSA and Azide free (ab281305)Sandwich ELISA detection of non-transfected and SARS-CoV-2 (COVID-19) Spike, MERS-CoV Spike, or SARS-CoV Spike transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts using ab281305 as capture antibody at concentration of 5 μg/mL and HRP-conjugated ab281304 as detection antibody at concentration of 1 μg/mL.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab281305 has not yet been referenced specifically in any publications.
