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Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

Reviews (1) Submit a question References (3)
Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Immunocytochemistry - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Immunocytochemistry - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

Key features and details

  • Mouse monoclonal [6H3] to SARS Nucleocapsid Protein
  • Suitable for: ICC, WB, IP, Sandwich ELISA
  • Isotype: IgG1

Overview

  • Product name

    Anti-SARS Nucleocapsid Protein antibody [6H3]
    See all SARS Nucleocapsid Protein primary antibodies

  • Description

    Mouse monoclonal [6H3] to SARS Nucleocapsid Protein

  • Host species

    Mouse

  • Specificity

    No cross-reactivity with MERS-CoV nucleocapsid protein is expected.

  • Tested applications

    Suitable for: ICC, WB, IP, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV, SARS-CoV-2
    Does not react with: MERS-CoV

  • Immunogen

    Recombinant fragment corresponding to Human coronavirus SARS Nucleocapsid Protein aa 121-422.

  • Positive control

    • IP: SARS CoV-2 Nucleocapsid Protein-transfected HEK-293T whole cell lysate. WB: SARS-CoV infected HEK-293T cell lysate; HEK-293T cells transfected with SARS CoV-2 Nucleocapsid Protein with DDDDK tag; Vero E6; SARS CoV and SARS CoV-2 infected Caco-2 cells whole cell extract. ICC: VeroE6 cells overexpressing SARS CoV Nucleocapsid Protein. SARS CoV-2 Nucleocapsid Protein transfected 293T cells.

  • General notes

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Constituent: 100% PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from tissue culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    6H3

  • Isotype

    IgG1

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab273434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
Use at an assay dependent concentration.
WB
1/1000 - 1/10000.
IP
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.
Notes
ICC
Use at an assay dependent concentration.
WB
1/1000 - 1/10000.
IP
Use at an assay dependent concentration.
Sandwich ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA.

  • Cellular localization

    Inside the virion, complexed with the viral RNA. May be associated with cellular membranes where it participates in viral RNA synthesis and virus budding.

  • Alternative names

    • N antibody
    • NC antibody
    • Nucleocapsid protein antibody
    • Nucleoprotein antibody
    • Protein N antibody
    • SARS coronavirus N protein antibody
    • SARS CoV antibody
    see all

Images

  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T cells at 30 µg
    Lane 2 : SARS-CoV NP transfected HEK-293T cells at 30 µg
    Lane 3 : SARS-CoV2 (COVID-19) NP transfected HEK-293T cells
    Lane 4 : MERS-CoV NP transfected HEK-293T cells at 30 µg


    10% SDS-PAGE

  • Immunocytochemistry - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Immunocytochemistry - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

    Immunocytochemistry analysis of Mock and SARS-CoV-2 Nucleocapsid transfected 293T cells labeling SARS Nucleocapsid Protein with ab273434 at 1/1000 dilution (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Nuclei counterstained with DAPI (blue).

  • Immunocytochemistry - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Immunocytochemistry - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

    VeroE6 cells over-expressing SARS-CoV Nucleocapsid protein labeling SARS Nucleocapsid Protein (green) using ab273434 in ICC. 

  • Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Sandwich ELISA - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

    Sandwich ELISA detection of recombinant full-length SARS-CoV-2 nucleocapsid protein using a different anti-SARS-CoV-2 capture antibody at concentration of 5 μg/mL and ab273434 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) was used at 1/10000  to detect the primary antibody.

  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution + SARS-CoV-2 (COVID-19) nucleocapsid protein at 0.5 µg

    Secondary
    HRP-conjugated anti-Mouse IgG


    12% SDS-PAGE

  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extract
    Lane 2 : SARS-CoV nucleocapsid-transfected (a partial fragment) HEK-293T whole cell extracts

    Lysates/proteins at 30 µg per lane.


    12% SDS-PAGE

  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extract
    Lane 2 : Transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-Mouse IgG


    10% SDS-PAGE

  • Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Immunoprecipitation - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

    SARS Nucleocapsid Protein was immunoprecipitated from 2μg of SARS-CoV2 Nucleocapsid Protein transfected HEK-293T whole cell lysate with either ab273434 (Lane 3) or a control IgG (Lane  2).

    Western blot was performed from the immunoprecipitate using ab273434. An

    HRP-conjugated anti mouse IgG antibody, was used as secondary antibody.

  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

    Lane 1 : SARS-CoV infected Vero E6 cells post infection after 0 hours
    Lane 2 : SARS-CoV infected Vero E6 cells post infection after 8 hours
    Lane 3 : SARS-CoV infected Vero E6 cells post infection after 16 hours
    Lane 4 : SARS-CoV infected Vero E6 cells post infection after 24 hours


    Vero E6  cells were analysed at the indicated time-points post-infection with SARS-CoV.

  • Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Western blot - Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
    Lanes 1-2 : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/1000 dilution
    Lane 3 : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)

    Lane 1 : Non-infected Caco-2 whole cell extract
    Lane 2 : SARS-CoV infected Caco-2 whole cell extract
    Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extract


    Cell lysates were analysed by WB 48 hours post-infection with SARS-CoV or SARS Cov-2 at a MOI of 0.01.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (3)

Publishing research using ab273434? Please let us know so that we can cite the reference in this datasheet.

ab273434 has been referenced in 3 publications.

  • Lv J  et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov 7:24 (2021). PubMed: 33850112
  • Diao B  et al. Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection. Nat Commun 12:2506 (2021). PubMed: 33947851
  • Xiang Q  et al. SARS-CoV-2 Induces Lymphocytopenia by Promoting Inflammation and Decimates Secondary Lymphoid Organs. Front Immunol 12:661052 (2021). PubMed: 33995382

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SARS Nucleocapsid Protein antibody [6H3]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (SARS-CoV-2 PCR positive human Lung section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 NaCitrate
Permeabilization
No
Specification
SARS-CoV-2 PCR positive human Lung section
Blocking step
H2O2 as blocking agent for 7 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

In Hwa Um

Verified customer

Submitted Jun 29 2020

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