Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
Key features and details
- Mouse monoclonal [6H3] to SARS Nucleocapsid Protein
- Suitable for: ICC, WB, IP, Sandwich ELISA
- Isotype: IgG1
Overview
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Product name
Anti-SARS Nucleocapsid Protein antibody [6H3]
See all SARS Nucleocapsid Protein primary antibodies -
Description
Mouse monoclonal [6H3] to SARS Nucleocapsid Protein -
Host species
Mouse -
Specificity
No cross-reactivity with MERS-CoV nucleocapsid protein is expected.
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Tested applications
Suitable for: ICC, WB, IP, Sandwich ELISAmore details -
Species reactivity
Reacts with: SARS-CoV, SARS-CoV-2
Does not react with: MERS-CoV -
Immunogen
Recombinant fragment corresponding to Human coronavirus SARS Nucleocapsid Protein aa 121-422.
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Positive control
- IP: SARS CoV-2 Nucleocapsid Protein-transfected HEK-293T whole cell lysate. WB: SARS-CoV infected HEK-293T cell lysate; HEK-293T cells transfected with SARS CoV-2 Nucleocapsid Protein with DDDDK tag; Vero E6; SARS CoV and SARS CoV-2 infected Caco-2 cells whole cell extract. ICC: VeroE6 cells overexpressing SARS CoV Nucleocapsid Protein. SARS CoV-2 Nucleocapsid Protein transfected 293T cells.
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General notes
Applications overview
Tick: Tested and Guaranteed to work X: Will not work —: No data
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
6H3 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273434 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent concentration.
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WB |
1/1000 - 1/10000.
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IP |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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ICC
Use at an assay dependent concentration. |
WB
1/1000 - 1/10000. |
IP
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Severe Acute Respiratory Syndrome (SARS), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus (SARS-CoV). SARS is caused by a human coronavirus, which are the major cause of upper respiratory tract illness in humans, such as the common cold. Coronaviruses are positive stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The spike protein is the main surface antigen of the coronavirus. The most prominent protein in the culture supernatants infected with SARS virus is a 46 kDa nucleocapsid protein. This suggests that the nucleocapsid protein is a major immunogen that may be useful for early diagnostics. The nucleocapsid protein of SARS shares little homology with nucleocapsid proteins of other members of the coronavirus family. Nucleocapsid proteins of other coronavirus have been reported to be involved in forming the viral core and also in the packaging and transcription of the viral RNA. -
Cellular localization
Inside the virion, complexed with the viral RNA. May be associated with cellular membranes where it participates in viral RNA synthesis and virus budding. -
Alternative names
- N antibody
- NC antibody
- Nucleocapsid protein antibody
see all
Images
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All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T cells at 30 µg
Lane 2 : SARS-CoV NP transfected HEK-293T cells at 30 µg
Lane 3 : SARS-CoV2 (COVID-19) NP transfected HEK-293T cells
Lane 4 : MERS-CoV NP transfected HEK-293T cells at 30 µg10% SDS-PAGE
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Immunocytochemistry analysis of Mock and SARS-CoV-2 Nucleocapsid transfected 293T cells labeling SARS Nucleocapsid Protein with ab273434 at 1/1000 dilution (green). Cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes. Nuclei counterstained with DAPI (blue).
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VeroE6 cells over-expressing SARS-CoV Nucleocapsid protein labeling SARS Nucleocapsid Protein (green) using ab273434 in ICC.
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Sandwich ELISA detection of recombinant full-length SARS-CoV-2 nucleocapsid protein using a different anti-SARS-CoV-2 capture antibody at concentration of 5 μg/mL and ab273434 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) was used at 1/10000 to detect the primary antibody.
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Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution + SARS-CoV-2 (COVID-19) nucleocapsid protein at 0.5 µg
Secondary
HRP-conjugated anti-Mouse IgG12% SDS-PAGE
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All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extract
Lane 2 : SARS-CoV nucleocapsid-transfected (a partial fragment) HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extract
Lane 2 : Transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-Mouse IgG10% SDS-PAGE
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SARS Nucleocapsid Protein was immunoprecipitated from 2μg of SARS-CoV2 Nucleocapsid Protein transfected HEK-293T whole cell lysate with either ab273434 (Lane 3) or a control IgG (Lane 2).
Western blot was performed from the immunoprecipitate using ab273434. An
HRP-conjugated anti mouse IgG antibody, was used as secondary antibody.
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All lanes : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
Lane 1 : SARS-CoV infected Vero E6 cells post infection after 0 hours
Lane 2 : SARS-CoV infected Vero E6 cells post infection after 8 hours
Lane 3 : SARS-CoV infected Vero E6 cells post infection after 16 hours
Lane 4 : SARS-CoV infected Vero E6 cells post infection after 24 hoursVero E6 cells were analysed at the indicated time-points post-infection with SARS-CoV.
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Lanes 1-2 : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434) at 1/1000 dilution
Lane 3 : Anti-SARS Nucleocapsid Protein antibody [6H3] (ab273434)
Lane 1 : Non-infected Caco-2 whole cell extract
Lane 2 : SARS-CoV infected Caco-2 whole cell extract
Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extractCell lysates were analysed by WB 48 hours post-infection with SARS-CoV or SARS Cov-2 at a MOI of 0.01.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (3)
ab273434 has been referenced in 3 publications.
- Lv J et al. Distinct uptake, amplification, and release of SARS-CoV-2 by M1 and M2 alveolar macrophages. Cell Discov 7:24 (2021). PubMed: 33850112
- Diao B et al. Human kidney is a target for novel severe acute respiratory syndrome coronavirus 2 infection. Nat Commun 12:2506 (2021). PubMed: 33947851
- Xiang Q et al. SARS-CoV-2 Induces Lymphocytopenia by Promoting Inflammation and Decimates Secondary Lymphoid Organs. Front Immunol 12:661052 (2021). PubMed: 33995382