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Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

Reviews (1) Submit a question References (14)
Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
  • Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

Key features and details

  • Mouse monoclonal [1A9] to SARS spike glycoprotein
  • Suitable for: ICC, WB, Flow Cyt, IP, Sandwich ELISA
  • Isotype: IgG1

Overview

  • Product name

    Anti-SARS spike glycoprotein antibody [1A9]
    See all SARS spike glycoprotein primary antibodies

  • Description

    Mouse monoclonal [1A9] to SARS spike glycoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC, WB, Flow Cyt, IP, Sandwich ELISAmore details

  • Species reactivity

    Reacts with: SARS-CoV, SARS-CoV-2

  • Immunogen

    Recombinant fragment within Human coronavirus SARS spike glycoprotein aa 1000-1200. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Infected Caco-2 cells; SARS-CoV-2 transfected HEK-293T cell lysate; SARS-Cov1 spike protein transfected Expi cell lysate, SARS-Cov1 3xFlag spike protein transfected Expi cell lysate, SARS-Cov2 spike protein transfected Expi cell lysate and SARS-Cov2 3xFlag spike protein transfected Expi cell lysate. ICC: SARS-CoV-2 transfected BHK-21, Vero E6, COS-7 cells. IP: SARS-CoV-2 transfected HEK-293T cells. Flow Cyt: Infected HEK-293T cells.

  • General notes

    Applications overview

    Tick: Tested and Guaranteed to work      X: Will not work      —: No data

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab273433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (1)
1/100 - 1/2000.
WB
1/500 - 1/3000. Predicted molecular weight: 139 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Sandwich ELISA
Use a concentration of 5 µg/ml.
Notes
ICC
1/100 - 1/2000.
WB
1/500 - 1/3000. Predicted molecular weight: 139 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Sandwich ELISA
Use a concentration of 5 µg/ml.

Target

  • Relevance

    A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.

  • Alternative names

    • E2 antibody
    • E2 glycoprotein antibody
    • Human coronavirus spike glycoprotein antibody
    • S antibody
    • S glycoprotein antibody
    • Spike glycoprotein antibody
    see all

Images

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lane 1 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Lanes 2-3 : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Mock
    Lane 2 : SARS-CoV infected Caco-2 whole cell extract
    Lane 3 : SARS-CoV-2 infected Caco-2 whole cell extract

    Predicted band size: 139 kDa

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1/1000 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extract
    Lane 2 : Transfected HEK-293T whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-mouse IgG antibody

    Predicted band size: 139 kDa

  • Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Western blot - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    All lanes : Anti-SARS spike glycoprotein antibody [1A9] (ab273433) at 1 µg/ml

    Lane 1 : Mock transfected human Expi cell lysate
    Lane 2 : PDL-1 Flag transfected human Expi cell lysate
    Lane 3 : SARS-Cov1 spike protein transfected human Expi cell lysate
    Lane 4 : SARS-Cov1 3xFlag spike protein transfected human Expi cell lysate
    Lane 5 : SARS-Cov2 spike protein transfected human Expi cell lysate
    Lane 6 : SARS-Cov2 3xFlag spike protein transfected human Expi cell lysate
    Lane 7 : MERS Spike protein transfected human Expi cell lysate
    Lane 8 : MERS 3xFlag Spike protein transfected human Expi cell lysate

    Lysates/proteins at 10 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 139 kDa
    Observed band size: 200 kDa why is the actual band size different from the predicted?



    Lanes 1 - 8: Merged signal (red and green). Green - ab273433 observed at 200 kDa. Red - loading control.

    ab273433 was shown to react with SARS spike glycoprotein in western blot. Membranes were blocked in 3% milk before incubation with ab273433 overnight at 4°C at 1 ug/ml. Blots were incubated with Donkey anti-Mouse IgG H&L(IRDye® 800CW) preabsorbed (ab216774) antibody at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    4% paraformaldehyde-fixed BHK-21 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC.

  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Methanol-fixed Vero E6 cells infected with SARS-CoV-2 (MOI of 1) stained for SARS-CoV-2 (green) using ab273433 at 1/2000 dilution in ICC. Counterstained with DAPI (Blue).

  • Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunocytochemistry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Mock and SARS-CoV-2-transfected COS-7 cells stained for SARS-CoV-2 (COVID-19) spike (green) using ab273433 at 1/2000 dilution in ICC. Nuclei were counterstained with DAPI (Blue).

  • Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Flow Cytometry - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Flow cytometric analysis of HEK-293T cells infected with recombinant vaccinia virus carrying the spike gene, using ab273433.

  • Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Sandwich ELISA - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    Sandwich ELISA detection of SARS spike glycoprotein using ab273433 as the capture antibody at 5 µg/mL. An alternative antibody was used as the detection antibody at 1 µg/mL followed by a Rabbit IgG antibody (HRP) at 1/10000 to detect the primary antibody.

  • Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)
    Immunoprecipitation - Anti-SARS spike glycoprotein antibody [1A9] (ab273433)

    SARS-CoV-2 Spike was immunoprecipitated from 2μg of HEK-293T whole cell lysate with ab273433. Western blot was performed from the immunoprecipitate using ab273433. EasyBlot HRP-conjugated anti mouse IgG antibody.


    Lane 1: HEK-293T lysate 2 μg (Input).
    Lane 2: Control IgG IP in HEK-293T lysate.
    Lane 3: ab273433 IP in HEK-293T lysate.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (14)

Publishing research using ab273433? Please let us know so that we can cite the reference in this datasheet.

ab273433 has been referenced in 14 publications.

  • Wang Z  et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol 19:210-221 (2022). PubMed: 34983944
  • Ohishi T  et al. Epigallocatechin gallate (EGCG) attenuates severe acute respiratory coronavirus disease 2 (SARS-CoV-2) infection by blocking the interaction of SARS-CoV-2 spike protein receptor-binding domain to human angiotensin-converting enzyme 2. PLoS One 17:e0271112 (2022). PubMed: 35830431
  • Ao Z  et al. SARS-CoV-2 Delta spike protein enhances the viral fusogenicity and inflammatory cytokine production. iScience 25:104759 (2022). PubMed: 35854977
  • Lieber CM  et al. SARS-CoV-2 VOC type and biological sex affect molnupiravir efficacy in severe COVID-19 dwarf hamster model. Nat Commun 13:4416 (2022). PubMed: 35906230
  • Dolton G  et al. Emergence of immune escape at dominant SARS-CoV-2 killer T cell epitope. Cell 185:2936-2951.e19 (2022). PubMed: 35931021
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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Immunocytochemistry abreview for Anti-SARS spike glycoprotein antibody [1A9]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry
Sample
SARS-CoV-2 Cultured Cells (Supernatant of Spodoptera frugiperda (Sf9) culture)
Permeabilization
No
Specification
Supernatant of Spodoptera frugiperda (Sf9) culture
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 04 2023

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