Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)
![Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)](https://www.abcam.com/ps/products/264/ab264431/Images/ab264431-459575-antisc35antibodysc35nuclearspecklemarkerimmunocytochemistryrin5frat.jpg "Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)")
Key features and details
- Mouse monoclonal [SC-35] to SC35 - BSA and Azide free
- Suitable for: ICC
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-SC35 antibody [SC-35] - BSA and Azide free
See all SC35 primary antibodies -
Description
Mouse monoclonal [SC-35] to SC35 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICCmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Xenopus laevis, Drosophila melanogaster, Rhesus monkey, Newt
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Immunogen
Other Immunogen Type. Fractioned spliceosome complex (PMID: 2137203)
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Epitope
This antibody recognizes a phospho-epitope of the non-snRNP (small nuclear ribonucleoprotein particles) factor SC35. The antibody reacts with the splicing factor SC-35 and with the SC-35-related non-snRNP factor SF2/ASF. -
Positive control
- ICC: MCF7, Rin-5F, and NIH/3T3 cells.
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General notes
ab264431 is the carrier-free version of ab11826.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
SC-35 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
|
ICC
Use at an assay dependent concentration. |
Target
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Function
Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway. -
Sequence similarities
Belongs to the splicing factor SR family.
Contains 1 RRM (RNA recognition motif) domain. -
Post-translational
modificationsExtensively phosphorylated on serine residues in the RS domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6427 Human
- Entrez Gene: 20382 Mouse
- Entrez Gene: 494445 Rat
- Entrez Gene: 708105 Rhesus monkey
- Omim: 600813 Human
- SwissProt: Q01130 Human
- SwissProt: Q62093 Mouse
- SwissProt: Q6PDU1 Rat
see all -
Alternative names
- 35 kDa antibody
- arginine/serine-rich 2 antibody
- PR264 antibody
see all
Images
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Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)
This data was developed using ab11826, the same antibody clone in a different buffer formulation.
ab11826 staining SC35 - Nuclear Speckle Marker in Rin-5F cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
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![Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)](https://www.abcam.com/ps/products/264/ab264431/Images/ab264431-459574-antisc35antibodysc35nuclearspecklemarkerimmunocytochemistrynih3t3mouse.jpg)
Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)This data was developed using ab11826, the same antibody clone in a different buffer formulation.
ab11826 staining SC35 - Nuclear Speckle Marker in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
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![Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)](https://www.abcam.com/ps/products/264/ab264431/Images/ab264431-459573-antisc35antibodysc35nuclearspecklemarkerimmunocytochemistrymcf7human.jpg)
Immunocytochemistry - Anti-SC35 antibody [SC-35] - BSA and Azide free (ab264431)This data was developed using ab11826, the same antibody clone in a different buffer formulation.
ab11826 staining SC35 - Nuclear Speckled Marker in MCF7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11826 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab264431 has not yet been referenced specifically in any publications.
