Recombinant Anti-SCAI antibody [EPR4128] (ab124688)

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Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4128] to SCAI
Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-SCAI antibody [EPR4128]
See all SCAI primary antibodies
Description

Rabbit monoclonal [EPR4128] to SCAI
Host species

Rabbit
Tested applications

Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human thymus, thyroid, and 293T cell lysates; SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 5.80 x 10 ^-12 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4128
Isotype

IgG
Research areas

Alternative Versions
- Anti-SCAI antibody [EPR4128] - BSA and Azide free (ab247960)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/5. See IHC antigen retrieval protocol.
Flow Cyt (Intra)		1/160. For unpurified, use 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		1/400. For unpurified, use 1/50.
WB		1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa). For unpurified, use 1/200.

Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/5. See IHC antigen retrieval protocol.
Flow Cyt (Intra) 1/160. For unpurified, use 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/400. For unpurified, use 1/50.
WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa). For unpurified, use 1/200.

Function

Tumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.
Sequence similarities

Belongs to the SCAI family.
Cellular localization

Membrane. Nucleus. Cytoplasm. Nuclear localization is required for inhibition of MKL1.
Target information above from: UniProt accession Q8N9R8 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 286205 Human
- Entrez Gene: 320271 Mouse
- Entrez Gene: 690538 Rat
- SwissProt: Q8N9R8 Human
- SwissProt: Q8C8N2 Mouse
- Unigene: 59504 Human
- Unigene: 204836 Mouse
Alternative names
- C9orf126 antibody
- FLJ11758 antibody
- FLJ36664 antibody
- MGC120525 antibody
- MGC120526 antibody
- MGC120528 antibody
- NET40 antibody
- Protein SCAI antibody
- SCAI antibody
- SCAI_HUMAN antibody
- Suppressor of cancer cell invasion antibody
- Suppressor of cancer cell invasion protein antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µg

Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)

Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] (ab124688)

Overlay histogram showingSH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)

Lane 1 : Human Thymus lysate
Lane 2 : Human Thyroid lysate
Lane 3 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 70 kDa
Western blot - Anti-SCAI antibody [EPR4128] (ab124688)

All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCAI antibody [EPR4128] (ab124688)

Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
Immunocytochemistry/ Immunofluorescence - Anti-SCAI antibody [EPR4128] (ab124688)

Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor^® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
Flow Cytometry (Intracellular) - Anti-SCAI antibody [EPR4128] (ab124688)

Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by intracellular flow cytometry (red) compared to a nonspecific negative control antibody (green).
OI-RD Scanning - Anti-SCAI antibody [EPR4128] (ab124688)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-SCAI antibody [EPR4128] (ab124688)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab124688? Please let us know so that we can cite the reference in this datasheet.

ab124688 has been referenced in 4 publications.

Zhang Y et al. Exosomal CircGDI2 Suppresses Oral Squamous Cell Carcinoma Progression Through the Regulation of MiR-424-5p/SCAI Axis. Cancer Manag Res 12:7501-7514 (2020). PubMed: 32943917
Zhao Z et al. Circular RNA Cdr1as Upregulates SCAI to Suppress Cisplatin Resistance in Ovarian Cancer via miR-1270 Suppression. Mol Ther Nucleic Acids 18:24-33 (2019). PubMed: 31479922
Zou Y et al. miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity. Mol Med Rep 15:1631-1637 (2017). ICC/IF ; Human . PubMed: 28260055
Krawczyk KM et al. Injury induced expression of caveolar proteins in human kidney tubules - role of megakaryoblastic leukemia 1. BMC Nephrol 18:320 (2017). PubMed: 29065889

Customer reviews and Q&As

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Conjugation kits

Isotype control

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (4)

Customer reviews and Q&As

Dissociation constant (K_D)