Name: Anti-Scavenging Receptor SR-BI antibody [EPR20190]
Brand: RabMAb
SKU: ab217318
Rating: 5 (2 reviews)

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Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20190] to Scavenging Receptor SR-BI
Suitable for: WB, IHC-P, ICC/IF, IP
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-Scavenging Receptor SR-BI antibody [EPR20190]
See all Scavenging Receptor SR-BI primary antibodies
Description

Rabbit monoclonal [EPR20190] to Scavenging Receptor SR-BI
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver lysate; Mouse liver, heart, kidney and spleen lysates; Rat liver lysate; U937, LNCaP, PC-3, THP-1, HepG2, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human liver, diffuse large B cell lymphoma and hepatocellular carcinoma tissues; Mouse liver tissue; Rat liver and cerebral cortex tissues. ICC/IF: HepG2 cells. IP: Human fetal liver lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20190
Isotype

IgG
Research areas

Alternative Versions
- Anti-Scavenging Receptor SR-BI antibody [EPR20190] - Low endotoxin, Azide free (ab222931)
- Anti-Scavenging Receptor SR-BI antibody [EPR20190] - BSA and Azide free (ab272003)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human Scavenging Receptor SR-BI protein (Fc Chimera) (ab182680)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab217318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/2000. Detects a band of approximately 82 kDa (predicted molecular weight: 60 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100.
IP		1/30.

Notes
WB 1/2000. Detects a band of approximately 82 kDa (predicted molecular weight: 60 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/30.

Function

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
Tissue specificity

Widely expressed.
Sequence similarities

Belongs to the CD36 family.
Post-translational
modifications

N-glycosylated.
Cellular localization

Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
Target information above from: UniProt accession Q8WTV0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 949 Human
- Entrez Gene: 20778 Mouse
- Entrez Gene: 25073 Rat
- Omim: 601040 Human
- SwissProt: Q8WTV0 Human
- SwissProt: Q61009 Mouse
- SwissProt: P97943 Rat
- Unigene: 520348 Human
- Unigene: 282242 Mouse
- Unigene: 88169 Rat
see all
Alternative names
- CD36 and LIMPII analogous 1 antibody
- CD36 antibody
- CD36 Antigen like 1 antibody
- CD36 antigen-like 1 antibody
- CD36L1 antibody
- CLA 1 antibody
- CLA-1 antibody
- CLA1 antibody
- Collagen type I receptor antibody
- HDLQTL6 antibody
- MGC138242 antibody
- SCARB1 antibody
- Scavebger Receptor Class B Member 1 antibody
- Scavenger receptor class B member 1 antibody
- Scavenger Receptor Class B Type 1 antibody
- SCRB1_HUMAN antibody
- SR BI antibody
- SR-BI antibody
- SRB1 antibody
- SRBI antibody
- Thrombospondin receptor like 1 antibody
- thrombospondin receptor-like 1 antibody
see all

Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : SCARB1 knockout HEK-293T cell lysate
Lane 3 : Human Liver cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 60 kDa
Observed band size: 70,75 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-Scavenging Receptor SR-BI antibody [EPR20190] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab217318 was shown to bind specifically to Scavenging Receptor SR-BI. A band was observed at 70/75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in SCARB1 knockout cell line ab282646 (knockout cell lysate ab283046). To generate this image, wild-type and SCARB1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Scavenging Receptor SR-BI knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Human liver whole cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab217318 observed at 80 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab217318 was shown to specifically react with Scavenging Receptor SR-BI in wild-type HAP1 cells as signal was lost in Scavenging Receptor SR-BI knockout cells. Wild-type and Scavenging Receptor SR-BI knockout samples were subjected to SDS-PAGE. Ab217318 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunofluorescent analysis of 100% methanol fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Scavenging Receptor SR-BI with ab217318 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HepG2 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594)) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

Lane 1 : Human fetal liver lysate at 20 µg
Lane 2 : Mouse liver lysate at 20 µg
Lane 3 : Rat liver lysate at 20 µg
Lane 4 : Mouse heart lysate at 10 µg
Lane 5 : Mouse kidney lysate at 10 µg
Lane 6 : Mouse spleen lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 60 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1-3: 4 seconds; Lane 4-6: 3 minutes.

Scavenging Receptor SR-BI undergoes N-glycosylation when it is expressed. The molecular weight observed is consistent with what has been described in the literature (PMID: 12016218; PMID: 10821819; PMID: 224442701).
Immunoprecipitation - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Scavenging Receptor SR-BI was immunoprecipitated from 0.35 mg of Human fetal liver lysate with ab217318 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217318 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human fetal liver lysate, 10 μg (Input).

Lane 2: ab217318 IP in Human fetal liver lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217318 in Human fetal liver lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

Lane 1 : U937 (Human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 4 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 60 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 30 seconds; Lane 2: 4 seconds; Lane 3: 2 seconds; Lane 4-5: 5 seconds.
Western blot - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

All lanes : Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 60 kDa
Observed band size: 82 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on human diffuse large B cell lymphoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human hepatocellular carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on rat liver is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunohistochemical analysis of paraffin-embedded rat cerebral cortex tissue labeling Scavenging Receptor SR-BI with ab217318 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous and cytoplasmic staining on rat cerebral cortex blood vessel endothelium is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Scavenging Receptor SR-BI antibody [EPR20190] (ab217318)

Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab217318? Please let us know so that we can cite the reference in this datasheet.

ab217318 has been referenced in 26 publications.

Palmer MA et al. Localisation and regulation of cholesterol transporters in the human hair follicle: mapping changes across the hair cycle. Histochem Cell Biol 155:529-545 (2021). PubMed: 33404706
An D et al. Reversal of Multidrug Resistance by Apolipoprotein A1-Modified Doxorubicin Liposome for Breast Cancer Treatment. Molecules 26:N/A (2021). PubMed: 33652957
Indyk D et al. Advanced glycation end products and their receptors in serum of patients with type 2 diabetes. Sci Rep 11:13264 (2021). PubMed: 34168187
Chen X et al. Hesperetin inhibits foam cell formation and promotes cholesterol efflux in THP-1-derived macrophages by activating LXRa signal in an AMPK-dependent manner. J Physiol Biochem 77:405-417 (2021). PubMed: 34212313
Wei C et al. HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry. Nat Metab 2:1391-1400 (2020). PubMed: 33244168

View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Application

Flow Cytometry

Sample

Human Cell (HEK 293)

Permeabilization

Gating Strategy

SS INT vs FS INT (cells) / FS INT vs FS TOF (Singlets)/ FL6 (Alexa 647) vs FL1 (GFP)/ SS INT vs FL1 INT (GFP+Cells)/ Count VS FL6 (Alexa 657)

Specification

HEK 293

Preparation

Cell harvesting/tissue preparation method: 2X105 cells/well in DMEM (24w/p)
Sample buffer: PBS1X BSA1%

Fixation

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Irene Garcia

Verified customer

Submitted Apr 26 2021

Application

Western blot

Sample

Human Cell lysate - whole cell (Fibroblasts (701 & 1386))

Gel Running Conditions

Reduced Denaturing (Gradient 4-20%)

Loading amount

20 µg

Treatment

siRNA Treatment (ScreenFect Protocol)

Specification

Fibroblasts (701 & 1386)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Ilse Kammerhofer

Verified customer

Submitted Feb 12 2020

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (26)

Customer reviews and Q&As

Flow Cytometry abreview for Anti-Scavenging Receptor SR-BI antibody [EPR20190]

Western blot abreview for Anti-Scavenging Receptor SR-BI antibody [EPR20190]

Post-translational
modifications