Recombinant Anti-SCD1 antibody [EPR21963] KO Tested (ab236868)

Product name

Anti-SCD1 antibody [EPR21963]
See all SCD1 primary antibodies
Description

Rabbit monoclonal [EPR21963] to SCD1
Host species

Rabbit
Specificity

ab236868 is recommended for human only in WB.
Tested applications

Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, HEK-293 and SK-MEL-28 whole cell lysate. IHC-P: Human adipose tissue within cardiac muscle tissue; Mouse adipose tissue of stomach tissue; Rat adipose tissue of pancreas tissue. ICC/IF: HepG2 and SK-MEL-28 cells. Flow: HepG2 cells. IP: HepG2 and HEK-293 whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21963
Isotype

IgG
Research areas

Alternative Versions
- Anti-SCD1 antibody [EPR21963] - BSA and Azide free (ab238171)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human SCD (SCD1) knockout HeLa cell line (ab265220)
KO cell lysates
- Human SCD (SCD1) knockout HeLa cell lysate (ab257658)
Related Products
- Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab236868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		1/500.
WB		1/1000. Predicted molecular weight: 42 kDa. ab236868 is recommended for human only in WB.
IHC-P		1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/100.
IP		1/30.

Notes
Flow Cyt (Intra) 1/500.
WB 1/1000. Predicted molecular weight: 42 kDa. ab236868 is recommended for human only in WB.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/100.
IP 1/30.

Function

Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
Sequence similarities

Belongs to the fatty acid desaturase family.
Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.
Cellular localization

Endoplasmic reticulum membrane.
Target information above from: UniProt accession O00767 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6319 Human
- Entrez Gene: 20249 Mouse
- Entrez Gene: 83792 Rat
- Omim: 604031 Human
- SwissProt: O00767 Human
- SwissProt: P13516 Mouse
- SwissProt: P07308 Rat
- Unigene: 558396 Human
- Unigene: 597496 Human
see all
Alternative names
- ACOD_HUMAN antibody
- Acyl-CoA desaturase antibody
- Delta(9)-desaturase antibody
- Delta-9 desaturase antibody
- Delta-9-Desaturase antibody
- FADS5 antibody
- Fatty acid desaturase antibody
- PRO0998 antibody
- SCD 1 antibody
- SCD antibody
- SCD1 antibody
- Stearoyl-CoA desaturase antibody
see all

Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)

All lanes : Anti-SCD1 antibody [EPR21963] (ab236868) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : SCD knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 42 kDa

anes 1-3: Merged signal (red and green). Green - ab236868. Red - loading control ab8245 observed at 50 kDa.

ab236868 Anti-SCD1 antibody [EPR21963] was shown to specifically react with SCD1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265220 (knockout cell lysate ab257658) was used. Wild-type and SCD1 knockout samples were subjected to SDS-PAGE. ab236868 and Anti-tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)

ab236868 staining SCD1 in wild-type HeLa cells, with negative expression in SCD1 knockout HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab236868 at 0.04 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 µg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor^® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

This product also work with 100% methanol (5 min) fixation under the same testing conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)

Immunohistochemical analysis of paraffin-embedded human adipose tissue within cardiac muscle tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human adipose cells in cardiac muscle (PMID: 15907797) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-SCD1 antibody [EPR21963] (ab236868)

All lanes : Anti-SCD1 antibody [EPR21963] (ab236868) at 1/1000 dilution

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : SK-MEL-28 (human malignant melanoma), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 42 kDa

Exposure time: 4 seconds

Blocking/dilution buffer: 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.

We recommend that customers do not boil samples to prevent protein aggregation.
Immunoprecipitation - Anti-SCD1 antibody [EPR21963] (ab236868)

SCD1 was immunoprecipitated from 0.35 mg of HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate with ab236868 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: HEK-293 whole cell lysate 10 μg (input).
Lane 2: ab236868 IP in HEK-293 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236868 in HEK-293 whole cell lysate (-).

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.
Flow Cytometry (Intracellular) - Anti-SCD1 antibody [EPR21963] (ab236868)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cell line labeling SCD1 with ab236868 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SCD1 with ab236868 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution. Nuclear counterstain is DAPI.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)

Immunohistochemical analysis of paraffin-embedded rat adipose tissue of pancreas tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in adipose cells in rat pancreas (PMID: 11500518) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCD1 antibody [EPR21963] (ab236868)

Immunohistochemical analysis of paraffin-embedded mouse adipose tissue of stomach tissue labeling SCD1 with ab236868 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in adipose cells in mouse stomach (PMID: 11500518) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunoprecipitation - Anti-SCD1 antibody [EPR21963] (ab236868)

SCD1 was immunoprecipitated from 10 μg of HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate with ab236868 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236868 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1: HepG2 whole cell lysate 10 μg (input).
Lane 2: ab236868 IP in HepG2 whole cell lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236868 in HepG2 whole cell lysate (-).

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 30 seconds.

The expression profile observed is consistent with what has been described in the literature (PMID: 20876744; PMID: 9843580; PMID: 17449569). The full-length protein migrates at 37 kDa; the 28 kDa fragment may represent an SCD1 cleavage product.
Immunocytochemistry/ Immunofluorescence - Anti-SCD1 antibody [EPR21963] (ab236868)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 (Human malignant melanoma) cells labeling SCD1 with ab236868 at 1/100 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in SK-MEL-28 cell line. Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 dilution. Nuclear counterstain is DAPI.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Anti-SCD1 antibody [EPR21963] (ab236868)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab236868? Please let us know so that we can cite the reference in this datasheet.

ab236868 has been referenced in 13 publications.

Sun R et al. Pink Lotus Essential Oil and Alleviates on Free Fatty Acid Induced Steatosis in HepG2 Cells via PI3K/Akt and NF-κB Pathways. J Oleo Sci 71:95-104 (2022). PubMed: 35013040
Zhang T et al. Dysregulated lipid metabolism blunts the sensitivity of cancer cells to EZH2 inhibitor. EBioMedicine 77:103872 (2022). PubMed: 35158113
Xiang J et al. LncRNA MALAT1 Promotes PPARα/CD36-Mediated Hepatic Lipogenesis in Nonalcoholic Fatty Liver Disease by Modulating miR-206/ARNT Axis. Front Bioeng Biotechnol 10:858558 (2022). PubMed: 35769097
Li R et al. Reducing VEGFB accelerates NAFLD and insulin resistance in mice via inhibiting AMPK signaling pathway. J Transl Med 20:341 (2022). PubMed: 35907871
Wang X et al. The downstream PPARγ target LRRC1 participates in early stage adipocytic differentiation. Mol Cell Biochem N/A:N/A (2022). PubMed: 36370237

View all Publications for this product

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Domain

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (13)

Customer reviews and Q&As