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    products/primary-antibodies/scdgfbpdgf-d-antibody-epr72662-ab181845.pdf

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RecombinantRabMAb

Recombinant Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (1) Submit a question References (2)

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Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
  • Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
  • Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-SCDGFB/PDGF-D antibody [EPR7266(2)]
    See all SCDGFB/PDGF-D primary antibodies
  • Description

    Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, fetal heart and small intestine lysate. PC-3 cell lysate. Human squamous cell lung carcinoma tissue. Mouse heart tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7266(2)
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • Other
    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Growth Factors/Hormones
    • PDGF
    • Neuroscience
    • Sensory System
    • Visual system
    • Cancer
    • Growth factors
    • PDGF

Associated products

  • Alternative Versions

    • Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] - BSA and Azide free (ab240960)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SCDGFB/PDGF-D protein (denatured) (ab134520)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181845 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. Activated by proteolytic cleavage and this active form acts as a specific ligand for beta platelet-derived growth factor receptor. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis.
  • Tissue specificity

    Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of arteriosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines.
  • Sequence similarities

    Belongs to the PDGF/VEGF growth factor family.
    Contains 1 CUB domain.
  • Developmental stage

    Not detectable in the earliest stages of glomerulogenesis, and not detected in the metanephric blastema or surrounding cortical interstitial cells. In later stages of glomerulogenesis, localized to epithelial cells transitioning from the early developing nephrons of the comma- and S-shaped stages to the visceral epithelial cells of differentiated glomeruli. In the developing pelvis, expressed at the basement membrane of immature collecting ducts and by presumptive fibroblastic cells in the interstitium.
  • Post-translational
    modifications

    Proteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9GZP0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 80310 Human
    • Entrez Gene: 71785 Mouse
    • Entrez Gene: 66018 Rat
    • Omim: 609673 Human
    • SwissProt: Q9GZP0 Human
    • SwissProt: Q925I7 Mouse
    • SwissProt: Q9EQT1 Rat
    • Unigene: 352298 Human
    • Unigene: 390122 Mouse
    • Unigene: 64493 Rat
    see all
  • Alternative names

    • IEGF antibody
    • Iris expressed growth factor antibody
    • Iris-expressed growth factor antibody
    • MGC26867 antibody
    • MSTP036 antibody
    • PDGF D antibody
    • PDGF-D antibody
    • PDGFD antibody
    • PDGFD latent form antibody
    • PDGFD receptor-binding form antibody
    • PDGFD_HUMAN antibody
    • Platelet derived growth factor D antibody
    • Platelet-derived growth factor D antibody
    • receptor-binding form antibody
    • rSCDGF-B antibody
    • SCDGF B antibody
    • SCDGF-B antibody
    • SCDGFB antibody
    • Spinal cord derived growth factor B antibody
    • Spinal cord-derived growth factor B antibody
    • spinal-cord derived growth factor-B protein antibody
    see all

Images

  • Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    All lanes : Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human small intestine lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)

    Predicted band size: 43 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

    Immunohistochemical analysis of paraffin-embedded Mouse heart tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Western blot - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution + PC3 cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 43 kDa

  • Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
    Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (2)

Publishing research using ab181845? Please let us know so that we can cite the reference in this datasheet.

ab181845 has been referenced in 2 publications.

  • Li X  et al. Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits. Nat Commun 11:2815 (2020). PubMed: 32499537
  • Jiang B  et al. Platelet-derived growth factor-D promotes colorectal cancer cell migration, invasion and proliferation by regulating Notch1 and matrix metalloproteinase-9. Oncol Lett 15:1573-1579 (2018). PubMed: 29434852

Customer reviews and Q&As

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCDGFB/PDGF-D antibody [EPR7266(2)]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human fetal membranes)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9
Permeabilization
Yes - 0.5% Tween
Specification
Human fetal membranes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Katharina Gegenschatz

Verified customer

Submitted Apr 20 2022

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