Recombinant Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SCDGFB/PDGF-D antibody [EPR7266(2)]
See all SCDGFB/PDGF-D primary antibodies -
Description
Rabbit monoclonal [EPR7266(2)] to SCDGFB/PDGF-D -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney, fetal heart and small intestine lysate. PC-3 cell lysate. Human squamous cell lung carcinoma tissue. Mouse heart tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7266(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/2000. Predicted molecular weight: 43 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. Activated by proteolytic cleavage and this active form acts as a specific ligand for beta platelet-derived growth factor receptor. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. -
Tissue specificity
Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of arteriosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family.
Contains 1 CUB domain. -
Developmental stage
Not detectable in the earliest stages of glomerulogenesis, and not detected in the metanephric blastema or surrounding cortical interstitial cells. In later stages of glomerulogenesis, localized to epithelial cells transitioning from the early developing nephrons of the comma- and S-shaped stages to the visceral epithelial cells of differentiated glomeruli. In the developing pelvis, expressed at the basement membrane of immature collecting ducts and by presumptive fibroblastic cells in the interstitium. -
Post-translational
modificationsProteolytic removal of the N-terminal CUB domain releasing the core domain is necessary for unmasking the receptor-binding epitopes of the core domain. Cleavage after Arg-247 or Arg-249 by urokinase plasminogen activator gives rise to the active form. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 80310 Human
- Entrez Gene: 71785 Mouse
- Entrez Gene: 66018 Rat
- Omim: 609673 Human
- SwissProt: Q9GZP0 Human
- SwissProt: Q925I7 Mouse
- SwissProt: Q9EQT1 Rat
- Unigene: 352298 Human
see all -
Alternative names
- IEGF antibody
- Iris expressed growth factor antibody
- Iris-expressed growth factor antibody
see all
Images
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All lanes : Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human small intestine lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Predicted band size: 43 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
Immunohistochemical analysis of paraffin-embedded Mouse heart tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845)
Immunohistochemical analysis of paraffin-embedded Human squamous cell lung carcinoma tissue labeling SCDGFB/PDGF-D using ab181845 at 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG prediluted was used as secondary. Counterstain: Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-SCDGFB/PDGF-D antibody [EPR7266(2)] (ab181845) at 1/1000 dilution + PC3 cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181845 has been referenced in 4 publications.
- Ma S et al. PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35027451
- Xu Z et al. Acetylation of Checkpoint suppressor 1 enhances its stability and promotes the progression of triple-negative breast cancer. Cell Death Discov 8:474 (2022). PubMed: 36450706
- Li X et al. Whole-genome resequencing of wild and domestic sheep identifies genes associated with morphological and agronomic traits. Nat Commun 11:2815 (2020). PubMed: 32499537
- Jiang B et al. Platelet-derived growth factor-D promotes colorectal cancer cell migration, invasion and proliferation by regulating Notch1 and matrix metalloproteinase-9. Oncol Lett 15:1573-1579 (2018). PubMed: 29434852