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Anti-SCGF beta antibody (ab9835)

Submit a review Q&A (1)References (1)

Key features and details

  • Goat polyclonal to SCGF beta
  • Suitable for: Sandwich ELISA, Neutralising, WB, ELISA
  • Reacts with: Human
  • Isotype: unknown

Overview

  • Product name

    Anti-SCGF beta antibody
    See all SCGF beta primary antibodies

  • Description

    Goat polyclonal to SCGF beta

  • Host species

    Goat

  • Tested applications

    Suitable for: Sandwich ELISA, Neutralising, WB, ELISAmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein corresponding to Human SCGF beta aa 1-350.
    Database link: Q9Y240

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Recombinant human SCGF beta protein (ab9836) can be used as a positive control in WB.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Lyophilized:Reconstitute with 200µl of sterile water.

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.40

    No preservative, sterile filtered
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    unknown

  • Light chain type

    unknown

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab9835 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use at an assay dependent concentration.

To detect Human SCGF-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required.
Neutralising
Use at an assay dependent concentration.

for one-half maximal inhibition [ND50] of the biological activity of hSCGFb (30.00 ng/ml).
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Allows the detection of 0.2 - 0.4 ng/well of recombinant hSCGFb.
Notes
Sandwich ELISA
Use at an assay dependent concentration.

To detect Human SCGF-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required.
Neutralising
Use at an assay dependent concentration.

for one-half maximal inhibition [ND50] of the biological activity of hSCGFb (30.00 ng/ml).
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Allows the detection of 0.2 - 0.4 ng/well of recombinant hSCGFb.

Target

  • Function

    Stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. Acts synergistically with other cytokines, including IL-3, GCSF, GMCSF and FLT3 ligand. Suppresses SCF-stimulated erythrocyte proliferation.

  • Tissue specificity

    Expressed in skeletal tissues including bone marrow, chondrocytes, primary ossification center-associated cells, the perichondrium and periosteum. Lower levels of expression were detected in spleen, thymus, appendix and fetal liver.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Developmental stage

    In the bone marrow, expression is limited to immature neutrophils. Expression was not detected in circulating mature neutrophils.

  • Post-translational
    modifications

    O-glycosylated. Probably sulfated on the O-glycans.

  • Cellular localization

    Cytoplasm. Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • C type lectin domain family 11 member A antibody
      • C type lectin superfamily member 3 antibody
      • C-type lectin domain family 11 member A antibody
      • C-type lectin superfamily member 3 antibody
      • CLC11_HUMAN antibody
      • CLEC11A antibody
      • CLECSF3 antibody
      • LSLCL antibody
      • Lymphocyte secreted C type lectin antibody
      • Lymphocyte secreted C-type lectin antibody
      • p47 antibody
      • Stem cell growth factor antibody
      see all

    References (1)

    Publishing research using ab9835? Please let us know so that we can cite the reference in this datasheet.

    ab9835 has been referenced in 1 publication.

    • Hiraoka A Leukemia cell lines require self-secreted stem cell growth factor (SCGF) for their proliferation. Leuk Res 32:1623-5 (2008). Human . PubMed: 18289675

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question


    I want to purchase antibody against SCGF-beta but when look carefully in the product list and surprised that there are two antibody has same specification but different product number.Will you please give the detailed difference between ab9835 and ab110493. Which one better and why?

    Read More
    Answer

    Thank you for contacting us. At Abcam we aim to provide our customers with a wide range of products to fit our researchers needs, and this somtimes results in very similar antibodies in our catalog. These two antibodies are both goat polyclonal with the same immunogen, recombinant hSCGFb, have the same purification and the same buffer (PBS). The difference is what applications they are tested for. Both are tested for WB and ELISA, however the antibody ab9835 has been tested for neutralising, while ab110493 has been tested for sandwich ELISA and has a matched pair.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

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