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Anti-SCP1 antibody (ab15090)

Reviews (3)Q&A (2)References (102)
Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody (ab15090)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody (ab15090)

Key features and details

  • Rabbit polyclonal to SCP1
  • Suitable for: IHC-Fr, IHC-P, ICC/IF
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-SCP1 antibody
    See all SCP1 primary antibodies

  • Description

    Rabbit polyclonal to SCP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide corresponding to Mouse SCP1 (C terminal).
    Database link: Q62209

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
IHC-P (1)
1/750.
ICC/IF (1)
1/100 - 1/750.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
1/750.
ICC/IF
1/100 - 1/750.

Target

  • Function

    Major component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase.

  • Tissue specificity

    Testis.

  • Domain

    Consists of an alpha-helical stretch of 700 AA residues, flanked by N- and C-terminal globular domains. The C-terminal domain has DNA-binding capacity.

  • Cellular localization

    Nucleus. Chromosome. In tripartite segments of synaptonemal complexes, between lateral elements in the nucleus. Found only where the chromosome cores are synapsed. Its N-terminus is found towards the center of the synaptonemal complex while the C-terminus extends well into the lateral domain of the synaptonemal complex.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cancer/testis antigen 8 antibody
      • CT8 antibody
      • HOM TES 14 antibody
      • MGC104417 antibody
      • SCP 1 antibody
      • SCP-1 antibody
      • SCP1 antibody
      • SYCP 1 antibody
      • Sycp1 antibody
      • SYCP1_HUMAN antibody
      • Synaptonemal complex protein 1 antibody
      • Testicular tissue protein Li 191 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody (ab15090)
      Immunocytochemistry/ Immunofluorescence - Anti-SCP1 antibody (ab15090)
      SCP1 labeled in mouse pachytene preparation (red), using ab15090. CDK2 staining, near teleomeres, is also present (green).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody (ab15090)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP1 antibody (ab15090)
      Paraffin embeded IHC image of Murine SCP1 in mouse ovary using ab15090 at 1:750 dilution and secondary at 1:400.

    References (102)

    Publishing research using ab15090? Please let us know so that we can cite the reference in this datasheet.

    ab15090 has been referenced in 102 publications.

    • Pereira C  et al. Multiple 9-1-1 complexes promote homolog synapsis, DSB repair, and ATR signaling during mammalian meiosis. Elife 11:N/A (2022). PubMed: 35133274
    • Tanno N  et al. FBXO47 is essential for preventing the synaptonemal complex from premature disassembly in mouse male meiosis. iScience 25:104008 (2022). PubMed: 35310947
    • Dang H  et al. Centrosome linker protein C-Nap1 maintains stem cells in mouse testes. EMBO Rep 23:e53805 (2022). PubMed: 35599622
    • Horisawa-Takada Y  et al. Meiosis-specific ZFP541 repressor complex promotes developmental progression of meiotic prophase towards completion during mouse spermatogenesis. Nat Commun 12:3184 (2021). PubMed: 34075040
    • Fang K  et al. Prediction and Validation of Mouse Meiosis-Essential Genes Based on Spermatogenesis Proteome Dynamics. Mol Cell Proteomics 20:100014 (2021). PubMed: 33257503
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-SCP1 antibody

     Excellent
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (testis)
    Permeabilization
    Yes - Triton
    Specification
    testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 21 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP1 antibody

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse testes)
    Specification
    mouse testes
    Fixative
    4% PFA
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 07 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SCP1 antibody

     Excellent
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Spermatocytes)
    Specification
    Spermatocytes
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 02 2007

    Question

    We ordered SCP1 antibody (ab15090) on Oct 6, 2009 by one person in our lab. When I used this antibody for meiotic spread staining recently, the antibody stained the whole X and Y chromosomes (it should stain only autosomes and only a small region on sex chromosomes). I checked the staining patterns published inDevelopment 139, 1133-1140 (2012, that SCP1 antibody indeed stained the whole sex chromosomes. So this antibody does not work appropriately. I found that you have another SCP1 antibody ab15087. Could you please send us some sample for trial? Thank you.

    Read More
    Answer

    Thank you for contacting Abcam.

    Under our Abpromise we guarantee our products for 6 months from the date of delivery and so it is no longer covered under this guarantee. I will be more than happy to help you troubleshoot your protocol to see if there are any changes that I can suggest that will help.

    Also, if you are concerned that the staining pattern is incorrect, I would recommend speaking to your lab colleague as to why they felt the staining was specific enough to publish with and also why they did not question the specificity of the antibody. Also were you using the exact same protocol as your lab colleague?

    Please let me know if there is anything else I can help you with.

    Read More

    Question

    I am a Chinese graduate student in Stomatology school of Wuhan University.I intend to use your antibodies of SCP-1 ab15090(mouse) SCP-1 ab15087(human), However SCP-1 ab15090 only can be applied in mouse in direction,SCP-1 ab15087 can be applied to ICC/IF,but I want to use SCP-1 antibody in immunohistochemistry of paraffin-embedded tumor samples from human beings.So I want to know which of them can be used in my research and whether or not SCP-1 (ab15090)can be applied to immunohistochemistry of paraffin-embedded tumor samples from human beings.I want to choose one of them. I look forward to your reply. Thank you very much.

    Read More
    Answer

    I can confirm that ab15090 is not likely to cross react with human as the immunogen shares only 83% homology with the human protein. Unfortunately ab15087 will work in human but does not appear to work in immunohistochemistry on paraffin-embedded sections. I'm sorry we don't appear to have a product suitable for your needs at the moment,

    Read More

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