Recombinant Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
![Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-489789-anti-scp3-antibody-cor-10g11-7-western-blot-mouse-rat-testis.jpg "Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Cor 10G11/7] to SCP3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-SCP3 antibody [Cor 10G11/7]
See all SCP3 primary antibodies -
Description
Mouse monoclonal [Cor 10G11/7] to SCP3 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Hamster, Zebrafish
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and Rat testis tissue lysates. IHC-P: Mouse and Rat testis tissue sections.
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General notes
This product has switched from a hybridoma to recombinant production method on 26 September 2022.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Cor 10G11/7 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
1/1000. Predicted molecular weight: 28 kDa.
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| Notes |
|---|
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IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/1000. Predicted molecular weight: 28 kDa. |
Target
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Function
Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. -
Tissue specificity
Testis-specific. -
Involvement in disease
Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. -
Sequence similarities
Belongs to the XLR/SYCP3 family. -
Cellular localization
Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. - Information by UniProt
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Database links
- Entrez Gene: 20962 Mouse
- Entrez Gene: 25561 Rat
- SwissProt: P70281 Mouse
- SwissProt: Q63520 Rat
- Unigene: 297977 Mouse
- Unigene: 34889 Rat
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Alternative names
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
see all
Images
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Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-489791-anti-scp3-antibody-cor-10g11-7-immunohistochemistry-rat-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-489790-anti-scp3-antibody-cor-10g11-7-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-343931-AntiSCP3-antibody-Cor-10G117-immunohistochemistry-E185-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)Shawki et al PLoS One. 2018 Jan 11;13(1):e0190800. doi: 10.1371/journal.pone.0190800. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/This image was generated from a previous batch made using the hybridoma production method.
Localization of MAFB in adult mouse testis.
Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-112251-anti-scp3-antibody-cor-10g11-7-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an abreview by Zachary Yu-Ching Lin.This image was generated from a previous batch made using the hybridoma production method.
IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.
The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)](https://www.abcam.com/ps/products/97/ab97672/Images/ab97672-168716-AntiSCP3-antibody-Cor-10G117-immunohistochemistry-mouse-adult-testis-tissue.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Qin WenThis image was generated from a previous batch made using the hybridoma production method.
ab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.
Datasheets and documents
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SDS download
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Datasheet download
References (144)
ab97672 has been referenced in 144 publications.
- Windley SP et al. Loss of NEDD4 causes complete XY gonadal sex reversal in mice. Cell Death Dis 13:75 (2022). PubMed: 35075134
- Pereira C et al. Multiple 9-1-1 complexes promote homolog synapsis, DSB repair, and ATR signaling during mammalian meiosis. Elife 11:N/A (2022). PubMed: 35133274
- Dai Y et al. Asynchronous embryonic germ cell development leads to a heterogeneity of postnatal ovarian follicle activation and may influence the timing of puberty onset in mice. BMC Biol 20:109 (2022). PubMed: 35550124
- Hirano K et al. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis. Commun Biol 5:504 (2022). PubMed: 35618762
- Li R et al. Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis 60:e23488 (2022). PubMed: 35765931