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    products/primary-antibodies/scp3-antibody-cor-10g117-ab97672.pdf

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Recombinant

Recombinant Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

  • Datasheet
  • SDS
Reviews (10) Submit a question References (115)

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Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [Cor 10G11/7] to SCP3
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

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Alexa Fluor® 488 Alexa Fluor® 647

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Overview

  • Product name

    Anti-SCP3 antibody [Cor 10G11/7]
    See all SCP3 primary antibodies
  • Description

    Mouse monoclonal [Cor 10G11/7] to SCP3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Hamster, Zebrafish
  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and Rat testis tissue lysates. IHC-P: Mouse and Rat testis tissue sections.
  • General notes

    This product has switched from a hybridoma to recombinant production method on 26 September 2022.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Cor 10G11/7
  • Myeloma

    Sp2/0-Ag14
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-SCP3 antibody [Cor 10G11/7] (ab205846)
    • Alexa Fluor® 647 Anti-SCP3 antibody [Cor 10G11/7] (ab205847)
    • Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (3)
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 28 kDa.
Notes
IHC-P
1/4000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 28 kDa.

Target

  • Function

    Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.
  • Tissue specificity

    Testis-specific.
  • Involvement in disease

    Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.
  • Sequence similarities

    Belongs to the XLR/SYCP3 family.
  • Cellular localization

    Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
  • Target information above from: UniProt accession Q8IZU3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 20962 Mouse
    • Entrez Gene: 25561 Rat
    • SwissProt: P70281 Mouse
    • SwissProt: Q63520 Rat
    • Unigene: 297977 Mouse
    • Unigene: 34889 Rat
    • Alternative names

      • choline phosphotransferase 1 antibody
      • chpt1 antibody
      • COR 1 antibody
      • COR1 antibody
      • MGC71888 antibody
      • RNASCP3 antibody
      • SCP 3 antibody
      • SCP-3 antibody
      • SCP3 antibody
      • SPGF4 antibody
      • Sycp 3 antibody
      • Sycp3 antibody
      • SYCP3_HUMAN antibody
      • Synaptonemal complex protein 3 antibody
      see all

    Images

    • Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      Western blot - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1/1000 dilution

      Lane 1 : Rat testis tissue lysate
      Lane 2 : Mouse testis tissue lysate

      Lysates/proteins at 20 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 28 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?


      Exposure time: 20 seconds


      Blocking and diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

      Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)Shawki et al PLoS One. 2018 Jan 11;13(1):e0190800. doi: 10.1371/journal.pone.0190800. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      This image was generated from a previous batch made using the hybridoma production method.

      Localization of MAFB in adult mouse testis.

      Double immunostaining of MAFB with SCP3 (ab97672 at a 1/400 dilution, O/N +4°C). Nuclei were counterstained with DAPI. The color of each marker is indicated above the images. All seminiferous tubules shown represent stage VII. US; undifferentiated spermatogonia. DS; differentiated spermatogonia. P; pachytene spermatocytes. Sp; spermatids. S; Sertoli cells. L; Leydig cells. MAFB was specifically detected in Leydig cells, Sertoli cells, and pachytene spermatocytes.

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an abreview by Zachary Yu-Ching Lin.

      This image was generated from a previous batch made using the hybridoma production method.

      IHC-P image of SCP3 staining with ab97672 on tissue sections from adult mouse testis.

      The sections were subjected to heat-mediated antigen retrieval using Dako antigen retrieval solution. The sections were then blocked with 5% milk for 30 minutes at 25°C, before incubation with ab97672 (1/200 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor®488 conjugated goat anti-mouse polyclonal, used at a 1/500 dilution.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] (ab97672)This image is courtesy of an Abreview submitted by Qin Wen

      This image was generated from a previous batch made using the hybridoma production method.

      ab97672 staining SCP3 in mouse adult testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

      Tissue was fixed with 4% paraformaldehyde and blocked with 5% BSA for 60 minutes at 25°C; antigen retrieval was by heat mediation in a 0.01M citrate buffer. Samples were incubated with primary antibody (1/200 in 1% BSA) for 1 hour at 22°C. A Biotin-conjugated Goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    Protocols

    • Mouse on Mouse staining protocol

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (115)

    Publishing research using ab97672? Please let us know so that we can cite the reference in this datasheet.

    ab97672 has been referenced in 115 publications.

    • Bagheri-Fam S  et al. Heterozygous deletion of Sox9 in mouse mimics the gonadal sex reversal phenotype associated with campomelic dysplasia in humans. Hum Mol Genet 29:3781-3792 (2021). PubMed: 33305798
    • Lopes F  et al. Chemotherapy induced damage to spermatogonial stem cells in prepubertal mouse in vitro impairs long-term spermatogenesis. Toxicol Rep 8:114-123 (2021). PubMed: 33425685
    • Wang X  et al. Cellular fate of intersex differentiation. Cell Death Dis 12:388 (2021). PubMed: 33846307
    • Yin H  et al. HDAC3 controls male fertility through enzyme-independent transcriptional regulation at the meiotic exit of spermatogenesis. Nucleic Acids Res 49:5106-5123 (2021). PubMed: 33939832
    • Liu Q  et al. Loss of CEP70 function affects acrosome biogenesis and flagella formation during spermiogenesis. Cell Death Dis 12:478 (2021). PubMed: 33980814
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 10 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Testis)
    Permeabilization
    Yes - 0.25% Triton X-100 for 5 minutes
    Specification
    Testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 10 2022

    IHC - Wholemount abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (Testis, Seminiferous Tubules)
    Specification
    Testis, Seminiferous Tubules
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Brad Hansen

    Verified customer

    Submitted Oct 07 2022

    Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis, adult)
    Permeabilization
    Yes - 0.1% Triton X-100 in PBS
    Specification
    Testis, adult
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Bryan Niedenberger

    Verified customer

    Submitted Sep 16 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Fish Tissue sections (testis)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: immunosaver 
    Specification
    testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 12 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Testis)
    Permeabilization
    No
    Specification
    Testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 22 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Chicken Cell (Ovaries)
    Permeabilization
    Yes - Triton X-100
    Specification
    Ovaries
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. xiaochuan tang

    Verified customer

    Submitted Jun 19 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-SCP3 [Cor 10G11/7] antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (postnatal 6 day testes)
    Specification
    postnatal 6 day testes
    Permeabilization
    No
    Fixative
    4% PFA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Qing Wen

    Verified customer

    Submitted Apr 04 2014

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (adult testes)
    Specification
    adult testes
    Fixative
    4% PFA
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citric acid/citrate sodium
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Qing Wen

    Verified customer

    Submitted Mar 18 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (adult mouse testis section)
    Specification
    adult mouse testis section
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Antigen retrieval solution
    Permeabilization
    No
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. 煜欽 林

    Verified customer

    Submitted Sep 24 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SCP3 antibody [Cor 10G11/7]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (embryonic day 16.5 female germ cells)
    Specification
    embryonic day 16.5 female germ cells
    Fixative
    Formaldehyde/SDS
    Permeabilization
    Yes - 0.05% Tween20 and 0.05% Triton X-100 in 1xPBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Jason Heaney

    Verified customer

    Submitted Sep 30 2011

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