Recombinant Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)
![Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)](https://www.abcam.com/ps/products/264/ab264093/Images/ab264093-492110-anti-scp3-antibody-cor-10g11-7-western-blot-mouse-rat-testis.jpg "Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Cor 10G11/7] to SCP3 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free
See all SCP3 primary antibodies -
Description
Mouse monoclonal [Cor 10G11/7] to SCP3 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Hamster, Zebrafish
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and Rat testis tissue lysates. IHC-P: Mouse and Rat testis tissue sections.
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General notes
ab264093 is the carrier-free version of ab97672.
This product has switched from a hybridoma to recombinant production method on 26 September 2022.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Cor 10G11/7 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa.
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 28 kDa. |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development. -
Tissue specificity
Testis-specific. -
Involvement in disease
Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes. -
Sequence similarities
Belongs to the XLR/SYCP3 family. -
Cellular localization
Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed. - Information by UniProt
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Database links
- Entrez Gene: 20962 Mouse
- Entrez Gene: 25561 Rat
- SwissProt: P70281 Mouse
- SwissProt: Q63520 Rat
- Unigene: 297977 Mouse
- Unigene: 34889 Rat
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Alternative names
- choline phosphotransferase 1 antibody
- chpt1 antibody
- COR 1 antibody
see all
Images
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Western blot - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)All lanes : Anti-SCP3 antibody [Cor 10G11/7] (ab97672) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 28 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?Blocking and diluting buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)](https://www.abcam.com/ps/products/264/ab264093/Images/ab264093-492109-anti-scp3-antibody-cor-10g11-7-immunohistochemistry-rat-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)](https://www.abcam.com/ps/products/264/ab264093/Images/ab264093-492108-anti-scp3-antibody-cor-10g11-7-immunohistochemistry-mouse-testis.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCP3 antibody [Cor 10G11/7] - BSA and Azide free (ab264093)Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab97672 at 1/4000 dilution followed by Leica DS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab97672 for 30 mins at room temperature and blocking mouse IgG by specific antibody (ab125913, 1:1000) for 8 mins. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab264093 has not yet been referenced specifically in any publications.
