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RecombinantRabMAb

Recombinant Anti-SCP3 antibody [EPR12695(B)] (ab181354)

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Flow Cytometry (Intracellular) - Anti-SCP3 antibody [EPR12695(B)] (ab181354)
  • Western blot - Anti-SCP3 antibody [EPR12695(B)] (ab181354)
  • Anti-SCP3 antibody [EPR12695(B)] (ab181354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12695(B)] to SCP3
  • Suitable for: WB, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SCP3 antibody [EPR12695(B)]
    See all SCP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR12695(B)] to SCP3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt (Intra)more details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human testis, Human seminoma, Jurkat and HepG2 lysates; Jurkat cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab181354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Component of the transverse filaments of synaptonemal complexes (SCS), formed between homologous chromosomes during meiotic prophase. Has an essential meiotic function in spermatogenesis. May be important for testis development.

  • Tissue specificity

    Testis-specific.

  • Involvement in disease

    Defects in SYCP3 are a cause of azoospermia due to perturbations of meiosis (AZSPM) [MIM:270960]. AZSPM is a condition of having no sperm present in the ejaculate. Testicular histology shows arrest of spermatogenesis at the pachytene stage of primary spermatocytes.

  • Sequence similarities

    Belongs to the XLR/SYCP3 family.

  • Cellular localization

    Nucleus. In tripartite segments of synaptonemal complexes, irrespective of whether these are synapsed or unsynapsed.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • choline phosphotransferase 1 antibody
    • chpt1 antibody
    • COR 1 antibody
    • COR1 antibody
    • MGC71888 antibody
    • RNASCP3 antibody
    • SCP 3 antibody
    • SCP-3 antibody
    • SCP3 antibody
    • SPGF4 antibody
    • Sycp 3 antibody
    • Sycp3 antibody
    • SYCP3_HUMAN antibody
    • Synaptonemal complex protein 3 antibody
    see all

Images

  • Flow Cytometry (Intracellular) - Anti-SCP3 antibody [EPR12695(B)] (ab181354)
    Flow Cytometry (Intracellular) - Anti-SCP3 antibody [EPR12695(B)] (ab181354)

    Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling SCP3with ab181354 at 1/10 dilution (red) compared toa rabbit IgG negative control (green). 

     

     

  • Western blot - Anti-SCP3 antibody [EPR12695(B)] (ab181354)
    Western blot - Anti-SCP3 antibody [EPR12695(B)] (ab181354)
    All lanes : Anti-SCP3 antibody [EPR12695(B)] (ab181354) at 1/1000 dilution

    Lane 1 : Human testis lysate
    Lane 2 : Human seminoma lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 28 kDa

  • Anti-SCP3 antibody [EPR12695(B)] (ab181354)
    Anti-SCP3 antibody [EPR12695(B)] (ab181354)

References (0)

Publishing research using ab181354? Please let us know so that we can cite the reference in this datasheet.

ab181354 has not yet been referenced specifically in any publications.

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