Anti-SDHB antibody [21A11AE7] (ab14714)
![Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)](https://www.abcam.com/ps/products/14/ab14714/Images/ab14714-396054-antiSDHBantibodyimmunocytochemistryimmunofluorescencehek293human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)")
Key features and details
- Mouse monoclonal [21A11AE7] to SDHB
- Suitable for: Flow Cyt, IHC-Fr, WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-SDHB antibody [21A11AE7]
See all SDHB primary antibodies -
Description
Mouse monoclonal [21A11AE7] to SDHB -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Hamster, Pig, Zebrafish
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Immunogen
Full length protein corresponding to Cow SDHB. Purified SDH from bovine heart.
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Positive control
- WB: Human, bovine, rat, and mouse heart mitochondria; Isolated mitochondria from HepG2 cells. IHC-Fr: Normal ageing human colon tissue. Flow Cyt: HEK-293 cells. ICC/IF: HeLa cells. HEK293 cells (HEK293-SDHB KO used as a negative cell line)
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
21A11AE7 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| IHC-Fr |
Use at an assay dependent concentration.
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| WB | (9) |
Use a concentration of 5 µg/ml. Detects a band of approximately 28 kDa.
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| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 5 µg/ml. Detects a band of approximately 28 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. -
Involvement in disease
Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. -
Sequence similarities
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 4Fe-4S ferredoxin-type domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 286840 Cow
- Entrez Gene: 6390 Human
- Entrez Gene: 67680 Mouse
- Entrez Gene: 414412 Pig
- Entrez Gene: 298596 Rat
- Entrez Gene: 562149 Zebrafish
- Omim: 185470 Human
- SwissProt: Q3T189 Cow
see all -
Alternative names
- CWS2 antibody
- DHSB_HUMAN antibody
- FLJ92337 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)
ab14714 staining SDHB in wild-type HEK293 cells (top panel) and SDHB knockout HEK293 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab14714 at 5µg concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Western blot - Anti-SDHB antibody [21A11AE7] (ab14714)](https://www.abcam.com/ps/products/14/ab14714/Images/ab14714-22054-anti-sdhb-antibody-21a11ae7-western-blot.jpg)
Western blot - Anti-SDHB antibody [21A11AE7] (ab14714)All lanes : Anti-SDHB antibody [21A11AE7] (ab14714) at 5 µg/ml
Lane 1 : Isolated mitochondria from Human heart at 5 µg
Lane 2 : Isolated mitochondria from Bovine Heart at 1 µg
Lane 3 : Isolated mitochondria from Rat heart at 10 µg
Lane 4 : Isolated mitochondria from Mouse heart at 10 µg
Lane 5 : Isolated mitochondria from HepG2 (human liver hepatocellular carcinoma cell line) cells at 20 µg
Secondary
All lanes : Goat anti-Mouse secondary
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs. -
![Immunohistochemistry (Frozen sections) - Anti-SDHB antibody [21A11AE7] (ab14714)](https://www.abcam.com/ps/products/14/ab14714/Images/ab14714-22050-anti-sdhb-antibody-21a11ae7-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-SDHB antibody [21A11AE7] (ab14714)ab14714 staining SDHB in normal ageing human colon tissue by Immunohistochemistry (Frozen sections). -
![Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)](https://www.abcam.com/ps/products/14/ab14714/Images/ab14714-22051-anti-sdhb-antibody-21a11ae7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)Mitochondrial localization of complex II visualized by immunocytochemistry using anti-complex II subunit 30 kDa Ip mAb 21A11 (ab14714). Cells were fixed, permeabilized and then labeled with ab14714 followed by an Alexa Fluor® 488-conjugated-goat-anti-mouse IgG2a isotype specific secondary antibody. -
![Flow Cytometry - Anti-SDHB antibody [21A11AE7] (ab14714)](https://www.abcam.com/ps/products/14/ab14714/Images/ab14714-22049-anti-sdhb-antibody-21a11ae7-flow-cytometry.jpg)
Flow Cytometry - Anti-SDHB antibody [21A11AE7] (ab14714)Overlay histogram showing HEK-293 (human epithelial cell line from embryonic kidney) cells stained with ab14714 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14714, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)](https://www.abcam.com/ps/products/14/ab14714/Images/ab14714-22052-anti-sdhb-antibody-21a11ae7-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SDHB antibody [21A11AE7] (ab14714)ICC/IF image of ab14714 stained HeLa (human epithelial cell line from cervix adenocarcinoma) cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14714, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
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SDS download
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Datasheet download
References (252)
ab14714 has been referenced in 252 publications.
- Pujol C et al. MPC2 variants disrupt mitochondrial pyruvate metabolism and cause an early-onset mitochondriopathy. Brain 146:858-864 (2023). PubMed: 36417180
- Silva-Pinheiro P et al. A library of base editors for the precise ablation of all protein-coding genes in the mouse mitochondrial genome. Nat Biomed Eng 7:692-703 (2023). PubMed: 36470976
- Maio N et al. Disruption of cellular iron homeostasis by IREB2 missense variants causes severe neurodevelopmental delay, dystonia and seizures. Brain Commun 4:fcac102 (2022). PubMed: 35602653
- Santana-Codina N et al. NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins. Cancer Discov 12:2180-2197 (2022). PubMed: 35771492
- Jia Z et al. Human TRUB1 is a highly conserved pseudouridine synthase responsible for the formation of Ψ55 in mitochondrial tRNAAsn, tRNAGln, tRNAGlu and tRNAPro. Nucleic Acids Res 50:9368-9381 (2022). PubMed: 36018806