Recombinant Anti-SDHB antibody [EPR13042(B)] (ab178423)
![Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-343308-Ab178423KOWBDOC.jpg "Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13042(B)] to SDHB
- Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SDHB antibody [EPR13042(B)]
See all SDHB primary antibodies -
Description
Rabbit monoclonal [EPR13042(B)] to SDHB -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, A431, HepG2 and Jurkat lysates; HepG2 cells; Human heart and kidney tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13042(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
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| IP |
1/10 - 1/100.
|
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa. |
|
IP
1/10 - 1/100. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1. -
Involvement in disease
Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus. -
Sequence similarities
Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
Contains 1 2Fe-2S ferredoxin-type domain.
Contains 1 4Fe-4S ferredoxin-type domain. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 6390 Human
- Entrez Gene: 67680 Mouse
- Entrez Gene: 298596 Rat
- Omim: 185470 Human
- SwissProt: P21912 Human
- SwissProt: Q9CQA3 Mouse
- SwissProt: P21913 Rat
- Unigene: 465924 Human
see all -
Alternative names
- CWS2 antibody
- DHSB_HUMAN antibody
- FLJ92337 antibody
see all
Images
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Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/5000 dilution
Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : SDHB knockout HEK-293 whole cell lysate
Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 32 kDaLanes 1 - 3: Merged signal (red and green). Green - ab178423 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.
ab178423 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab178423 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-206387-anti-sdhb-antibody-epr13042b-western-blot.jpg)
Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HepG2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 32 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-206386-anti-sdhb-antibody-epr13042b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SDHB with ab178423 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-206385-anti-sdhb-antibody-epr13042b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SDHB with ab178423 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-206384-anti-sdhb-antibody-epr13042b-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling SDHB with ab178423 at 1/10 dilution (red) compared with a rabbit IgG negative control (green).
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![Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-206383-anti-sdhb-antibody-epr13042b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)Western blot analysis on immunoprecipitation pellet from (1) A431 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178423 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
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![Anti-SDHB antibody [EPR13042(B)] (ab178423)](https://www.abcam.com/ps/products/178/ab178423/Images/ab178423-7-benefits-of-recombinant-antibodies.png)
Anti-SDHB antibody [EPR13042(B)] (ab178423)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab178423 has been referenced in 10 publications.
- Al Khazal F et al. Unexpected obesity, rather than tumorigenesis, in a conditional mouse model of mitochondrial complex II deficiency. FASEB J 35:e21227 (2021). PubMed: 33247500
- Su C & Wang F Clinical and molecular findings in a family expressing a novel heterozygous variant of the G elongation factor mitochondrial 1 gene. Exp Ther Med 20:173 (2020). PubMed: 33093908
- Zhang W et al. Functional validation of a human GLUD2 variant in a murine model of Parkinson's disease. Cell Death Dis 11:897 (2020). PubMed: 33093440
- Zhou X et al. LncRNA-BLACAT1 Facilitates Proliferation, Migration and Aerobic Glycolysis of Pancreatic Cancer Cells by Repressing CDKN1C via EZH2-Induced H3K27me3. Front Oncol 10:539805 (2020). PubMed: 33072570
- Sun G et al. Romo1 is involved in the immune response of glioblastoma by regulating the function of macrophages. Aging (Albany NY) 12:1114-1127 (2020). PubMed: 31945745
- Dukinfield M et al. Repurposing an anti-cancer agent for the treatment of hypertrophic heart disease. J Pathol 249:523-535 (2019). PubMed: 31424556
- Al Khazal F et al. A conditional mouse model of complex II deficiency manifesting as Leigh-like syndrome. FASEB J 33:13189-13201 (2019). PubMed: 31469588
- Chang HC et al. Bhlhe40 differentially regulates the function and number of peroxisomes and mitochondria in myogenic cells. Redox Biol 20:321-333 (2019). PubMed: 30391825
- Li H et al. Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts. Sci Rep 8:5118 (2018). PubMed: 29572489
- Her YF et al. Oxygen concentration controls epigenetic effects in models of familial paraganglioma. PLoS One 10:e0127471 (2015). WB ; Mouse, Human . PubMed: 25985299