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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-SDHB antibody [EPR13042(B)] (ab178423)

Reviews (1) Submit a question References (14)
Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)
  • Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)
  • Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)
  • Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)
  • Anti-SDHB antibody [EPR13042(B)] (ab178423)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13042(B)] to SDHB
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC Carrier Free HRP PE

Overview

  • Product name

    Anti-SDHB antibody [EPR13042(B)]
    See all SDHB primary antibodies

  • Description

    Rabbit monoclonal [EPR13042(B)] to SDHB

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal liver, A431, HepG2 and Jurkat lysates; HepG2 cells; Human heart and kidney tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab178423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 32 kDa.
IP
1/10 - 1/100.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.

Target

  • Function

    Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).

  • Pathway

    Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.

  • Involvement in disease

    Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
    Defects in SDHB are the cause of hereditary paragangliomas type 4 (PGL4) [MIM:115310]; also known as familial non-chromaffin paragangliomas type 4. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL4 is characterized by the development of mostly benign, highly vascular, slow growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors.
    Defects in SDHB are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
    Defects in SDHB are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.

  • Sequence similarities

    Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family.
    Contains 1 2Fe-2S ferredoxin-type domain.
    Contains 1 4Fe-4S ferredoxin-type domain.

  • Cellular localization

    Mitochondrion inner membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CWS2 antibody
    • DHSB_HUMAN antibody
    • FLJ92337 antibody
    • Ip antibody
    • Iron sulfur subunit antibody
    • Iron sulfur subunit of complex II antibody
    • Iron-sulfur subunit of complex II antibody
    • mitochondrial antibody
    • PGL 4 antibody
    • PGL4 antibody
    • SDH 1 antibody
    • SDH antibody
    • SDH1 antibody
    • SDH2 antibody
    • SDH2, homolog of antibody
    • SdhB antibody
    • SDHIP antibody
    • Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
    • Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody
    • Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
    • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
    • Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
    • Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
    • Succinate dehydrogenase complex iron sulfur subunit B antibody
    • Succinate dehydrogenase complex subunit B iron sulfur antibody
    • Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
    • succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
    • Succinate dehydrogenase iron sulfur protein antibody
    see all

Images

  • Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : SDHB knockout HEK-293 whole cell lysate
    Lane 3 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 32 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab178423 observed at 32 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab178423 was shown to recognize SDHB in wild-type HEK 293 cells as signal was lost at the expected MW in SDHB knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SDHB knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab178423 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Western blot - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    All lanes : Anti-SDHB antibody [EPR13042(B)] (ab178423) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 32 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling SDHB with ab178423 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SDHB with ab178423 at 1/50 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Flow Cytometry (Intracellular) - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Intracellular flow cytometric analysis of permeabilized HepG2 cells labeling SDHB with ab178423 at 1/10 dilution (red) compared with a rabbit IgG negative control (green). 

     

     

  • Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Immunoprecipitation - Anti-SDHB antibody [EPR13042(B)] (ab178423)

    Western blot analysis on immunoprecipitation pellet from (1) A431 cell lysate or (2) 1X PBS (negative control) immunoprecipitated using ab178423 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

  • Anti-SDHB antibody [EPR13042(B)] (ab178423)
    Anti-SDHB antibody [EPR13042(B)] (ab178423)

References (14)

Publishing research using ab178423? Please let us know so that we can cite the reference in this datasheet.

ab178423 has been referenced in 14 publications.

  • Antony R  et al. UCHL1 Regulates Lipid and Perilipin 2 Level in Skeletal Muscle. Front Physiol 13:855193 (2022). PubMed: 35464088
  • Thomas GE  et al. The metabolic enzyme hexokinase 2 localizes to the nucleus in AML and normal haematopoietic stem and progenitor cells to maintain stemness. Nat Cell Biol 24:872-884 (2022). PubMed: 35668135
  • Al Khazal F  et al. Unexpected obesity, rather than tumorigenesis, in a conditional mouse model of mitochondrial complex II deficiency. FASEB J 35:e21227 (2021). PubMed: 33247500
  • Tang Y  et al. Nicotinamide ameliorates energy deficiency and improves retinal function in Cav-1-/- mice. J Neurochem 157:550-560 (2021). PubMed: 33305362
  • Gui M  et al. Huoxue Qianyang Qutan recipe attenuates Ang II-induced cardiomyocyte hypertrophy by regulating reactive oxygen species production. Exp Ther Med 22:1446 (2021). PubMed: 34721688
View all Publications for this product

Customer reviews and Q&As

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Western blot abreview for Anti-SDHB antibody [EPR13042(B)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Gel Running Conditions
Reduced Denaturing (10 % gel)
Loading amount
10 µg
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Louise Reynolds

Verified customer

Submitted Jun 19 2019

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