Recombinant Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)](https://www.abcam.com/ps/products/240/ab240137/Images/ab240137-3-ab155999283921ab155999IHC1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11035(B)] to SDHC - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free
See all SDHC primary antibodies -
Description
Rabbit monoclonal [EPR11035(B)] to SDHC - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab240137 is the carrier-free version of ab155999.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11035(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa.
The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
|
|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.
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|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle. -
Involvement in disease
Defects in SDHC are the cause of hereditary paragangliomas type 3 (PGL3) [MIM:605373]; also known as autosomal dominant non-chromaffin paragangliomas type 3. Non-chromaffin paragangliomas are usually benign, neural crest derived tumors of parasympathetic ganglia.
Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also known as Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. -
Sequence similarities
Belongs to the cytochrome b560 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 6391 Human
- Entrez Gene: 66052 Mouse
- Entrez Gene: 289217 Rat
- Omim: 602413 Human
- SwissProt: Q99643 Human
- SwissProt: Q9CZB0 Mouse
- Unigene: 444472 Human
- Unigene: 198138 Mouse
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Alternative names
- CYB560 antibody
- C560_HUMAN antibody
- CYBL antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling SDHC with purified ab155999 at 1:700 dilution (0.2 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155999).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)](https://www.abcam.com/ps/products/240/ab240137/Images/ab240137-2-SDHCPrimaryantibodiesab1559992.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)](https://www.abcam.com/ps/products/240/ab240137/Images/ab240137-1-SDHCPrimaryantibodiesab1559993.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137) -
![Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)](https://www.abcam.com/ps/products/240/ab240137/Images/ab240137-4-benefits-of-recombinant-antibodies.png)
Anti-SDHC antibody [EPR11035(B)] - BSA and Azide free (ab240137)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab240137 has not yet been referenced specifically in any publications.
