Anti-SDHC antibody - N-terminal (ab191362)
Key features and details
- Rabbit polyclonal to SDHC - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SDHC antibody - N-terminal
See all SDHC primary antibodies -
Description
Rabbit polyclonal to SDHC - N-terminal -
Host species
Rabbit -
Specificity
ab191362 does not cross react with other proteins. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human SDHC aa 1-100 (N terminal).
Database link: Q99643 -
Positive control
- HeLa whole cell lysate; rat and mouse liver tissue lysate. Rat gaster and Human gastric cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 2.5% BSA, 0.45% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191362 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 19 kDa.
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| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 19 kDa. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). -
Pathway
Carbohydrate metabolism; tricarboxylic acid cycle. -
Involvement in disease
Defects in SDHC are the cause of hereditary paragangliomas type 3 (PGL3) [MIM:605373]; also known as autosomal dominant non-chromaffin paragangliomas type 3. Non-chromaffin paragangliomas are usually benign, neural crest derived tumors of parasympathetic ganglia.
Defects in SDHC are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also known as Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance. -
Sequence similarities
Belongs to the cytochrome b560 family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 327696 Cow
- Entrez Gene: 6391 Human
- Entrez Gene: 66052 Mouse
- Entrez Gene: 100524676 Pig
- Entrez Gene: 289217 Rat
- Omim: 602413 Human
- SwissProt: P35720 Cow
- SwissProt: Q99643 Human
see all -
Alternative names
- CYB560 antibody
- C560_HUMAN antibody
- CYBL antibody
see all
Images
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Western blot - Anti-SDHC antibody - N-terminal (ab191362)All lanes : Anti-SDHC antibody - N-terminal (ab191362) at 0.5 µg/ml
Lane 1 : Rat Liver Tissue Lysate
Lane 2 : Mouse Liver Tissue Lysate
Lane 3 : HeLa Whole Cell Lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 19 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (ab191362)Immunohistochemical analysis of paraffin-embedded rat gaster tissue, labeling SDHC using ab191362 at 1 μg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SDHC antibody - N-terminal (ab191362)Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue, labeling SDHC using ab191362 at 1 μg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab191362 has not yet been referenced specifically in any publications.