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RecombinantRabMAb

Recombinant Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
  • Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-SEC23B + SEC23A antibody [EPR22723-102]

  • Description

    Rabbit monoclonal [EPR22723-102] to SEC23B + SEC23A

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Purified LIF/His-tagged SEC23B recombinant protein, His-tagged SEC23A recombinant protein; human liver lysate; mouse liver lysate; MEF, HeLa, Raji, C6, RAW 264.7, PC-12, and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum tissue; mouse liver tissue, rat kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270750 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on rat kidney (PMID: 22745161).

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    All lanes : Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

    Lane 1 : MEF (mouse embryonic fibroblast (immortalized)) whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : Raji (human burkitts lymphoma b lymphocyte) whole cell lysate
    Lane 4 : Mouse liver tissue lysate
    Lane 5 : C6 (rat glial tumor glial cell) whole cell lysate
    Lane 6 : RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
    Lane 7 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 8 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 mg/ml

    Predicted band size: 86 kDa
    Observed band size: 86 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).

    Exposure time: Lanes 1-4: 26 seconds;Lanes 5-8: 10 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on mouse liver (PMID: 22745161).

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution + Human liver tissue lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 86 kDa
    Observed band size: 86 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 22745161).

    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SEC23B + SEC23A with ab270750 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive cytoplasmic staining on human cerebrum (PMID: 22745161) is observed.

    The section was incubated with ab270750 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Western blot - Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    All lanes : Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750) at 1/1000 dilution

    Lane 1 : Purified LIF/His-tagged SEC23B recombinant protein (aa127-392), 10 ng
    Lane 2 : His-tagged SEC23A recombinant protein (aa127-392)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 86 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Both the recombinant proteins were made in house.

    SEC23B recombinant protein was expressed from a mammalian - HEK-293 expression system. The LIF tag is about 40 kDa.

    SEC23A recombinant protein was from E.coil extracts. 

    Exposure time: 3 seconds.

  • Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)
    Anti-SEC23B + SEC23A antibody [EPR22723-102] (ab270750)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab270750? Please let us know so that we can cite the reference in this datasheet.

ab270750 has not yet been referenced specifically in any publications.

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