Recombinant Anti-SEC62 antibody [EPR9213] (ab140644)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9213] to SEC62
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SEC62 antibody [EPR9213]
See all SEC62 primary antibodies -
Description
Rabbit monoclonal [EPR9213] to SEC62 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human SEC62 aa 250-350. The exact sequence is proprietary.
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Positive control
- HeLa, SH-SY5Y, HepG2, NIH/3T3 and C6 cell lysates. Human kidney tissue and HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9213 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 46 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Antigen retrieval is recommended. |
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ICC/IF |
1/250 - 1/500.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended. |
ICC/IF
1/250 - 1/500. |
Target
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Function
Required for preprotein translocation. -
Sequence similarities
Belongs to the SEC62 family. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 7095 Human
- Entrez Gene: 69276 Mouse
- Entrez Gene: 294912 Rat
- Omim: 602173 Human
- SwissProt: Q99442 Human
- SwissProt: Q8BU14 Mouse
- Unigene: 592561 Human
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Alternative names
- Dtrp1 antibody
- FLJ32803 antibody
- hTP-1 antibody
see all
Images
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All lanes : Anti-SEC62 antibody [EPR9213] (ab140644) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : SH-SY5Y cell lysates
Lane 3 : HepG2 cell lysates
Lane 4 : NIH/3T3 cell lysates
Lane 5 : C6 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa -
Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labelling SEC62 with ab140644 at a 1/250 dilution.
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Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SEC62 with ab140644 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab140644 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140644 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab140644 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Normal Human Liver tissue using ab140644 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab140644 has been referenced in 4 publications.
- Murie M et al. ATase inhibition rescues age-associated proteotoxicity of the secretory pathway. Commun Biol 5:173 (2022). PubMed: 35217767
- Rigby MJ et al. Endoplasmic reticulum acetyltransferases Atase1 and Atase2 differentially regulate reticulophagy, macroautophagy and cellular acetyl-CoA metabolism. Commun Biol 4:454 (2021). PubMed: 33846551
- Wu J et al. Sec62 Regulates Endoplasmic Reticulum Stress and Autophagy Balance to Affect Foot-and-Mouth Disease Virus Replication. Front Cell Infect Microbiol 11:707107 (2021). PubMed: 34532300
- Han S et al. Sec62 Suppresses Foot-and-Mouth Disease Virus Proliferation by Promotion of IRE1a-RIG-I Antiviral Signaling. J Immunol 203:429-440 (2019). PubMed: 31167774