Recombinant Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)
![Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)](https://www.abcam.com/ps/products/275/ab275393/Images/ab275393-1-antisemaphorin-4dcd100antibody-epr2386669-flow-cytometry-pc3.jpg "Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23866-69] to Semaphorin 4D/CD100
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Semaphorin 4D/CD100 antibody [EPR23866-69]
See all Semaphorin 4D/CD100 primary antibodies -
Description
Rabbit monoclonal [EPR23866-69] to Semaphorin 4D/CD100 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HDLM-2 whole cell lysate. Flow cyt: HDLM-2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23866-69 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab275393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/500.
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| WB |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa).
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| Notes |
|---|
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Flow Cyt
1/500. |
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WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 96 kDa). |
Target
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Function
May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro. -
Tissue specificity
Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10507 Human
- Omim: 601866 Human
- SwissProt: Q92854 Human
- Unigene: 494406 Human
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Alternative names
- A8 antibody
- BB 18 antibody
- BB18 antibody
see all
Images
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Flow Cytometry - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)
Flow cytometric analysis of PC-3 (human prostate adenocarcinoma epithelial cell, Left) / HDLM-2(Human Hodgkin lymphoma, Right) cells labelling Semaphorin 4D/CD100 with ab275393 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: PC-3 (PMID: 23775445).
Gated on viable cells.
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![Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)](https://www.abcam.com/ps/products/275/ab275393/Images/ab275393-410979-antisemaphorin-4dcd100antibody-epr2386669-western-blot-lymphoma.jpg)
Western blot - Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)All lanes : Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393) at 1/1000 dilution
Lane 1 : HDLM-2 (Human Hodgkin lymphoma) whole cell lysate
Lane 2 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 23775445).
Negative control: PC-3 (PMID: 23775445)
Exposure time: 136 seconds.
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![Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)](https://www.abcam.com/ps/products/275/ab275393/Images/ab275393-3-benefits-of-recombinant-antibodies.png)
Anti-Semaphorin 4D/CD100 antibody [EPR23866-69] (ab275393)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab275393 has not yet been referenced specifically in any publications.